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Research article
Apolonia Sieprawska
Pedagogical University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2700-2044
License:
This work is licensed under a CC BY 4.0 License. Read Full License
BACKGROUND
Among the environmental stressors, which oxidative action is currently widely studied, are mycotoxins. The aim of presented studies was to investigate the response of leaves of seedlings to zearalenone (ZEN) applied directly to leaves and to grains (indirectly) of tolerant and sensitive wheat cultivars.
RESULTS
Biochemical analyses of antioxidative activity were performed for chloroplasts and showed the similar decrease of this activity independently of plant sensitivity and the way of ZEN application. On the other hand, higher amounts of superoxide radicals (microscopic observation) were formed in leaves of plants grown from grains incubated in solution of ZEN and in sensitive variety. Electron paramagnetic resonance (EPR) studies showed that upon ZEN treatment larger amount of Mn - aqua complexes was formed in leaves of tolerant wheat cultivar than in those of sensitive one whereas the degradation of Fe-protein complexes occurred independently of the sensitivity of plant varieties.
CONCLUSION
The changes in the quantity of organic stable radicals, which were formed by stabilizing reactive oxygen species on biochemical macromolecules, pointed to the higher ability to their generation in leaf tissues subjected to foliar ZEN treatment indicating the important role of these radical species in protective mechanism mainly against direct toxin action. The way of activation the defense mechanisms depended on the method of applications toxin.
Keywords
zearalenone, EPR, wheat seedlings, infection, redox homeostasis
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Apolonia Sieprawska
Pedagogical University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2700-2044
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 15 Mar, 2021
No community comments so far
Submission checks complete
On 07 Mar, 2021
Editor invited
On 02 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
BACKGROUND
Among the environmental stressors, which oxidative action is currently widely studied, are mycotoxins. The aim of presented studies was to investigate the response of leaves of seedlings to zearalenone (ZEN) applied directly to leaves and to grains (indirectly) of tolerant and sensitive wheat cultivars.
RESULTS
Biochemical analyses of antioxidative activity were performed for chloroplasts and showed the similar decrease of this activity independently of plant sensitivity and the way of ZEN application. On the other hand, higher amounts of superoxide radicals (microscopic observation) were formed in leaves of plants grown from grains incubated in solution of ZEN and in sensitive variety. Electron paramagnetic resonance (EPR) studies showed that upon ZEN treatment larger amount of Mn - aqua complexes was formed in leaves of tolerant wheat cultivar than in those of sensitive one whereas the degradation of Fe-protein complexes occurred independently of the sensitivity of plant varieties.
CONCLUSION
The changes in the quantity of organic stable radicals, which were formed by stabilizing reactive oxygen species on biochemical macromolecules, pointed to the higher ability to their generation in leaf tissues subjected to foliar ZEN treatment indicating the important role of these radical species in protective mechanism mainly against direct toxin action. The way of activation the defense mechanisms depended on the method of applications toxin.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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