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Jianxia Wen
Chengdu University of Traditional Chinese Medicine
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1184-8758
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Background: This study was aimed to investigate the therapeutic effects and potential mechanism of higenamine combined with [6]-gingerol (HG/[6]-GR) against doxorubicin (DOX) - induced chronic heart failure (CHF) in rats.
Materials and methods: Therapeutic effects of HG/[6]-GR on hemodynamics indices, serum biochemical indicators, histopathology and TUNEL staining of rats were assessed. Moreover, a UHPLC-Q-TOF/MS-based serum metabolic approach was performed to identify the metabolites and possible pathways of HG/[6]-GR on DOX-induced CHF.
Results: HG/[6]-GR had effects on promoting of hemodynamic indices, alleviating serum biochemical indicators, improving the pathological characteristics of heart tissue and reducing the apoptosis of myocardial cells. Serum metabolisms analyses indicated that the therapeutic effects of HG and [6]-GR were mainly associated with the regulation of eight metabolites, including acetylphosphate, 3-Carboxy-1-hydroxypropylthiamine diphosphate, coenzyme A, palmitic acid, PE(O-18:1(1Z)/20:4(5Z,8Z,11Z,14Z)), oleic acid, lysoPC(18:1(9Z)), and PC(16:0/16:0). Pathway analysis showed that the treatment of HG/[6]-GR on CHF treatment was related to twelve pathways, including glycerophospholipid metabolism, fatty acid metabolism, pantothenate and CoA biosynthesis, citrate cycle (TCA cycle), pyruvate metabolism, and arachidonic acid metabolism. Serum metabolites and metabolic pathways regulated by HG/[6]-GR appear to be related to energy metabolism.
Conclusion: Multivariate statistical analysis has provided new insights for understanding CHF and investigating the therapeutic effects and mechanisms of HG/[6]-GR, which influencing the metabolites and pathways related to energy metabolism pathway.
Keywords
Higenamine, [6]-Gingerol, Metabolomics, Chronic heart failure, Energy metabolism, Molecular mechanisms
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CURRENT STATUS: Published
View the published article at Chinese Medicine.
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Posted 28 Oct, 2020
No community comments so far
Editorial decision: Accept
On 08 Nov, 2020
Editor assigned
On 22 Oct, 2020
Submission checks complete
On 21 Oct, 2020
Editor invited
On 21 Oct, 2020
No comments provided
Review #2 received
Received 07 Oct, 2020
Reviewer #2 agreed
On 05 Oct, 2020
Reviewers invited
Invitations sent on 27 Sep, 2020
Reviewer #1 agreed
On 27 Sep, 2020
Review #1 received
Received 27 Sep, 2020
Editor assigned
On 25 Sep, 2020
Submission checks complete
On 24 Sep, 2020
Editor invited
On 24 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 16 Sep, 2020
Review #2 received
Received 23 Aug, 2020
Reviewer #2 agreed
On 16 Aug, 2020
Review #1 received
Received 15 Jun, 2020
Reviewer #1 agreed
On 12 Jun, 2020
Reviewers invited
Invitations sent on 02 Jun, 2020
Editor assigned
On 26 May, 2020
First submitted
On 25 May, 2020
Submission checks complete
On 25 May, 2020
Editor invited
On 25 May, 2020
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Chinese Medicine.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Jianxia Wen
Chengdu University of Traditional Chinese Medicine
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1184-8758
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Chinese Medicine.
Version 3
Posted 28 Oct, 2020
No community comments so far
Editorial decision: Accept
On 08 Nov, 2020
Editor assigned
On 22 Oct, 2020
Submission checks complete
On 21 Oct, 2020
Editor invited
On 21 Oct, 2020
No comments provided
Review #2 received
Received 07 Oct, 2020
Reviewer #2 agreed
On 05 Oct, 2020
Reviewers invited
Invitations sent on 27 Sep, 2020
Reviewer #1 agreed
On 27 Sep, 2020
Review #1 received
Received 27 Sep, 2020
Editor assigned
On 25 Sep, 2020
Submission checks complete
On 24 Sep, 2020
Editor invited
On 24 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 16 Sep, 2020
Review #2 received
Received 23 Aug, 2020
Reviewer #2 agreed
On 16 Aug, 2020
Review #1 received
Received 15 Jun, 2020
Reviewer #1 agreed
On 12 Jun, 2020
Reviewers invited
Invitations sent on 02 Jun, 2020
Editor assigned
On 26 May, 2020
First submitted
On 25 May, 2020
Submission checks complete
On 25 May, 2020
Editor invited
On 25 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Chinese Medicine.
Background: This study was aimed to investigate the therapeutic effects and potential mechanism of higenamine combined with [6]-gingerol (HG/[6]-GR) against doxorubicin (DOX) - induced chronic heart failure (CHF) in rats.
Materials and methods: Therapeutic effects of HG/[6]-GR on hemodynamics indices, serum biochemical indicators, histopathology and TUNEL staining of rats were assessed. Moreover, a UHPLC-Q-TOF/MS-based serum metabolic approach was performed to identify the metabolites and possible pathways of HG/[6]-GR on DOX-induced CHF.
Results: HG/[6]-GR had effects on promoting of hemodynamic indices, alleviating serum biochemical indicators, improving the pathological characteristics of heart tissue and reducing the apoptosis of myocardial cells. Serum metabolisms analyses indicated that the therapeutic effects of HG and [6]-GR were mainly associated with the regulation of eight metabolites, including acetylphosphate, 3-Carboxy-1-hydroxypropylthiamine diphosphate, coenzyme A, palmitic acid, PE(O-18:1(1Z)/20:4(5Z,8Z,11Z,14Z)), oleic acid, lysoPC(18:1(9Z)), and PC(16:0/16:0). Pathway analysis showed that the treatment of HG/[6]-GR on CHF treatment was related to twelve pathways, including glycerophospholipid metabolism, fatty acid metabolism, pantothenate and CoA biosynthesis, citrate cycle (TCA cycle), pyruvate metabolism, and arachidonic acid metabolism. Serum metabolites and metabolic pathways regulated by HG/[6]-GR appear to be related to energy metabolism.
Conclusion: Multivariate statistical analysis has provided new insights for understanding CHF and investigating the therapeutic effects and mechanisms of HG/[6]-GR, which influencing the metabolites and pathways related to energy metabolism pathway.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
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