The use of toxic and persistent pesticides in agriculture results in serious and lasting environmental impacts. Although traditional methods such as physical and chemical reclamation give the best results, treating these contaminants requires high cost and expertise. Therefore, this study focuses on bioremediation recovery, which is more efficient, economical, and safer to remove. In this case, the newly isolated potential of Pichia kluyveri FM012 in degrading 4-bromophenol was investigated. The selected strain was isolated from the tropical rainforest in Selangor, Malaysia. The impact of optimized parameters such as agitation, pH, nitrogen and carbon source were also studied. After extensive testing, the best optimal degradation was at pH 5 with 150 rpm stirrer speed. Glucose and yeast performed best compared to other carbon and nitrogen sources. The maximum biosorption capacity (qm = 38.46 mg/g biomass) was predicted by the Langmuir model, but the Freundlich model adsorption gave a better value of R2 = 0.999. The pseudo-second-order kinetic model fits the study of biosorption kinetics. The FTIR spectrum revealed the presence of asymmetric and symmetric vibration of the aromatic ring and was assigned C=C or C=O, and fungi showed biosorption ability in broad functional groups. These results provided interesting information about the ability of Pichia kluyveri FM012 and potential applications to remediate the resistant pesticide.