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Research article
Sri Widia A. Jusman
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8169-6825
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This work is licensed under a CC BY 4.0 License. Read Full License
Abstract Background Tissue regeneration is a process that need a high demand of oxygen and energy supply. Cytoglobin (Cygb) is a hexacoordinate globin superfamily that possesses strong oxygen-binding ability. Cygb also has a role in preventing cells from oxidative stress and carrying oxygen into the mitochondria. The production of energy for regeneration is associated with mitochondria, especially mitochondrial biogenesis. The peroxisome proliferator-activated receptor gamma coactivator (PGC-1α) is aprotein that plays an important role in regulating mitochondrial biogenesis. The house gecko (Hemidactylus platyurus) is a reptile with high ability for tissue regeneration in its tail. The house gecko was selected as the animal model for this research to analyse the role of Cygb which is associated with oxygen supply and PGC-1α that is related to energy production in tissue regeneration. Results The curve for the tail growth showed three different phases. Cygb mRNA was highly expressed during tissue regeneration. PGC-1α mRNA was expressed earlier than Cygb, with a lower expression but still higher than the control. Conclusions During the tail tissue regeneration process of the house gecko, the expression of Cygb and PGC-1α were dynamic and relatively higher than their expression observed in the control. Cygb and PGC-1α were suspected to have a significant role in the tissue regeneration process.
Keywords
hypoxia, mitochondrial biogenesis, PGC-1α, Cygb, house gecko tail tissue regeneration
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CURRENT STATUS: Published
View the published article at BMC Developmental Biology.
No community comments so far
Editorial decision: Accept
On 15 Apr, 2020
Editor assigned
On 13 Apr, 2020
Submission checks complete
On 12 Apr, 2020
Editor invited
On 12 Apr, 2020
No comments provided
Editorial decision: Minor revision
On 10 Apr, 2020
Review #1 received
Received 08 Apr, 2020
Review #2 received
Received 29 Mar, 2020
Reviewer #2 agreed
On 13 Mar, 2020
Reviewer #1 agreed
On 12 Mar, 2020
Reviewers invited
Invitations sent on 10 Mar, 2020
Editor assigned
On 04 Mar, 2020
Submission checks complete
On 03 Mar, 2020
Editor invited
On 03 Mar, 2020
Version 1
Posted 02 Aug, 2019
No comments provided
Editorial decision: Major revision
On 07 Jan, 2020
Review #2 received
Received 23 Dec, 2019
Reviewer #2 agreed
On 02 Dec, 2019
Review #1 received
Received 10 Oct, 2019
Reviewer #1 agreed
On 27 Sep, 2019
Reviewers invited
Invitations sent on 24 Aug, 2019
Editor invited
On 30 Jul, 2019
Editor assigned
On 25 Jul, 2019
Submission checks complete
On 24 Jul, 2019
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Subject Areas
Developmental Biology
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A new preprint design is coming!
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See the published version of this preprint at BMC Developmental Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Sri Widia A. Jusman
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8169-6825
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Developmental Biology.
No community comments so far
Editorial decision: Accept
On 15 Apr, 2020
Editor assigned
On 13 Apr, 2020
Submission checks complete
On 12 Apr, 2020
Editor invited
On 12 Apr, 2020
No comments provided
Editorial decision: Minor revision
On 10 Apr, 2020
Review #1 received
Received 08 Apr, 2020
Review #2 received
Received 29 Mar, 2020
Reviewer #2 agreed
On 13 Mar, 2020
Reviewer #1 agreed
On 12 Mar, 2020
Reviewers invited
Invitations sent on 10 Mar, 2020
Editor assigned
On 04 Mar, 2020
Submission checks complete
On 03 Mar, 2020
Editor invited
On 03 Mar, 2020
Version 1
Posted 02 Aug, 2019
No comments provided
Editorial decision: Major revision
On 07 Jan, 2020
Review #2 received
Received 23 Dec, 2019
Reviewer #2 agreed
On 02 Dec, 2019
Review #1 received
Received 10 Oct, 2019
Reviewer #1 agreed
On 27 Sep, 2019
Reviewers invited
Invitations sent on 24 Aug, 2019
Editor invited
On 30 Jul, 2019
Editor assigned
On 25 Jul, 2019
Submission checks complete
On 24 Jul, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Developmental Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Developmental Biology.
Abstract Background Tissue regeneration is a process that need a high demand of oxygen and energy supply. Cytoglobin (Cygb) is a hexacoordinate globin superfamily that possesses strong oxygen-binding ability. Cygb also has a role in preventing cells from oxidative stress and carrying oxygen into the mitochondria. The production of energy for regeneration is associated with mitochondria, especially mitochondrial biogenesis. The peroxisome proliferator-activated receptor gamma coactivator (PGC-1α) is aprotein that plays an important role in regulating mitochondrial biogenesis. The house gecko (Hemidactylus platyurus) is a reptile with high ability for tissue regeneration in its tail. The house gecko was selected as the animal model for this research to analyse the role of Cygb which is associated with oxygen supply and PGC-1α that is related to energy production in tissue regeneration. Results The curve for the tail growth showed three different phases. Cygb mRNA was highly expressed during tissue regeneration. PGC-1α mRNA was expressed earlier than Cygb, with a lower expression but still higher than the control. Conclusions During the tail tissue regeneration process of the house gecko, the expression of Cygb and PGC-1α were dynamic and relatively higher than their expression observed in the control. Cygb and PGC-1α were suspected to have a significant role in the tissue regeneration process.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
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