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Research article
Fikresilasie Samuel Tasew
Ethiopian Public Health Institute
Corresponding Author
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Background: Moringa stenopetala is belongs to flowering family Moringaceae and genus Moringa. It is often referred to as the East African Moringa tree because it is native only to southern Ethiopia and northern Kenya. The expansion of its cultivation and utilization throughout the world especially in Africa is becoming important. For such expansion, the existing propagation method is limiting, so it needs good propagation system to supply enough planting material with uniform genotype. Therefore, the main objective of this study was to optimize an in vitro shoot multiplication protocol for M. stenopetala by using shoot tip as explants.
Results: Shoots were sterilized and cultured on Muraghige and Skoog (MS) medium for in vitro shoot initiation. For multiple shoot induction, the explants were cultured on MS medium supplemented with different concentrations of kinetin (0.5, 1.0, 1.5, 2.0, 2.5 mg/l) along with Indole-3- butyric acid (IBA) or α -naphthalene acetic acid (NAA) (0.01, 0.1, 0.5mg/l) and maintained at 25 ± 2°C for four weeks. Rooting was achieved by culturing well developed shoots in half strength MS medium containing IBA (0.1, 0.5, 1.0, 1.5, 2.0 mg/l), NAA (0.1, 0.5, 1.0, 1.5, 2.0 mg/l) and 0.5 mg/l IBA in combination with NAA (0.1, 0.5, 1.0, 1.5, 2.0 mg/l). Statistical analysis revealed that there was significant difference among all treatments applied in both shoot multiplication and rooting experiments. Maximum number of shoots per explant (3.43±1.41) and 7.97±4.18 leaves per explant were obtained on MS medium containing 0.5 mg/l kinetin in combination with 0.01mg/l NAA. The highest mean number of roots per shoot (1.63±1.03) and mean root length (0.87±1.22 cm) were obtained on MS medium containing 1.0 mg/l NAA and 0.1 mg/l IBA alone respectively. After acclimatization, 76% plants survived in greenhouse.
Conclusions: In general, using NAA along with kinetin for shoot multiplication was better than kinetin along with IBA and application of NAA alone at concentration of 1.0 mg/l and 1.0 mg/l NAA along with 0.5 mg/l IBA were more effective for root induction.
Keywords
In vitro propagation, Moringa stenopetala, rooting, shoot multiplication
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View the published article at BMC Biotechnology.
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A new preprint design is coming!
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See the published version of this preprint at BMC Biotechnology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Fikresilasie Samuel Tasew
Ethiopian Public Health Institute
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Biotechnology.
Version 1
Posted 11 Jun, 2020
No community comments so far
Editorial decision: Revise before sending to peer reviewers
On 10 Jun, 2020
Editor assigned
On 01 Jun, 2020
Editor invited
On 31 May, 2020
Submission checks complete
On 31 May, 2020
First submitted
On 29 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biotechnology and Bioengineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Biotechnology.
Background: Moringa stenopetala is belongs to flowering family Moringaceae and genus Moringa. It is often referred to as the East African Moringa tree because it is native only to southern Ethiopia and northern Kenya. The expansion of its cultivation and utilization throughout the world especially in Africa is becoming important. For such expansion, the existing propagation method is limiting, so it needs good propagation system to supply enough planting material with uniform genotype. Therefore, the main objective of this study was to optimize an in vitro shoot multiplication protocol for M. stenopetala by using shoot tip as explants.
Results: Shoots were sterilized and cultured on Muraghige and Skoog (MS) medium for in vitro shoot initiation. For multiple shoot induction, the explants were cultured on MS medium supplemented with different concentrations of kinetin (0.5, 1.0, 1.5, 2.0, 2.5 mg/l) along with Indole-3- butyric acid (IBA) or α -naphthalene acetic acid (NAA) (0.01, 0.1, 0.5mg/l) and maintained at 25 ± 2°C for four weeks. Rooting was achieved by culturing well developed shoots in half strength MS medium containing IBA (0.1, 0.5, 1.0, 1.5, 2.0 mg/l), NAA (0.1, 0.5, 1.0, 1.5, 2.0 mg/l) and 0.5 mg/l IBA in combination with NAA (0.1, 0.5, 1.0, 1.5, 2.0 mg/l). Statistical analysis revealed that there was significant difference among all treatments applied in both shoot multiplication and rooting experiments. Maximum number of shoots per explant (3.43±1.41) and 7.97±4.18 leaves per explant were obtained on MS medium containing 0.5 mg/l kinetin in combination with 0.01mg/l NAA. The highest mean number of roots per shoot (1.63±1.03) and mean root length (0.87±1.22 cm) were obtained on MS medium containing 1.0 mg/l NAA and 0.1 mg/l IBA alone respectively. After acclimatization, 76% plants survived in greenhouse.
Conclusions: In general, using NAA along with kinetin for shoot multiplication was better than kinetin along with IBA and application of NAA alone at concentration of 1.0 mg/l and 1.0 mg/l NAA along with 0.5 mg/l IBA were more effective for root induction.
Figure 1
Figure 2
Figure 3
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