See the published version of this preprint at Molecular Medicine.
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Research article
Huimin Hu
Affiliated Hospital of Hubei University of Arts and Science
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1737-6333
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background The expression of SIN3A is closely correlated with EA treatment efficacy of SIA, but its underlying mechanisms remain to be further explored.
Methods Quantitative real-time PCR was performed to analyze the expression of candidate miRNAs and SIN3A mRNA in a rat model of SIA. Western blot was carried out to evaluate the differential expression of SIN3A proteins under distinct circumstances. Luciferase assay was used to explore the inhibitory role of certain miRNAs in SIN3A expression. NOR test was performed to assess the memorial ability of SIA rats undergoing EA treatment. Immunohistochemistry was carried out to evaluate the expression of SIN3A in the hippocampus of SIA rats.
Results Rno-miR-183-5p, rno-miR-34c-3p and rno-miR-210-3p were significantly up-regulated in SIA rats treated with EA. In addition, rno-miR-183-5p and rno-miR-210-3p exerted an inhibitory effect on SIN3A expression. EA treatment of SIA rats effectively restored the normal expression of rno-miR-183-5p, rno-miR-210-3p and SIN3A. The NOR test also confirmed the effect of EA treatment on the improvement of memory in SIA rats.
Conclusion In this study, an animal model of SIA was treated with EA to investigate its therapeutic effect. Moreover, our work presented solid evidence on the regulatory pathway of miR-183/SIN3A and miR-210/SIN3A in the pathogenesis of SIA.
Keywords
Electroacupuncture, scopolamine, SIN3A, miR-210, miR-183, recognitive impairment.
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On 24 Aug, 2020
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On 03 Aug, 2020
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Version 1
Posted 28 May, 2020
No comments provided
Editorial decision: Major revision
On 28 Jun, 2020
Review #2 received
Received 27 Jun, 2020
Review #1 received
Received 24 Jun, 2020
Reviewer #2 agreed
On 13 Jun, 2020
Reviewer #1 agreed
On 10 Jun, 2020
Reviewers invited
Invitations sent on 09 Jun, 2020
Editor invited
On 02 Jun, 2020
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Subject Areas
Molecular Genetics
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See the published version of this preprint at Molecular Medicine.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Huimin Hu
Affiliated Hospital of Hubei University of Arts and Science
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1737-6333
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Molecular Medicine.
No community comments so far
Editor assigned
On 20 Oct, 2020
Editorial decision: Accept
On 20 Oct, 2020
Submission checks complete
On 19 Oct, 2020
Editor invited
On 19 Oct, 2020
No comments provided
Editor assigned
On 05 Oct, 2020
Editorial decision: Minor revision
On 05 Oct, 2020
Submission checks complete
On 04 Oct, 2020
Editor invited
On 04 Oct, 2020
No comments provided
Editor assigned
On 15 Sep, 2020
Submission checks complete
On 14 Sep, 2020
Editor invited
On 14 Sep, 2020
No comments provided
Editorial decision: Major revision
On 24 Aug, 2020
Review #1 received
Received 22 Aug, 2020
Editor assigned
On 03 Aug, 2020
Reviewers invited
Invitations sent on 03 Aug, 2020
Reviewer #1 agreed
On 03 Aug, 2020
Submission checks complete
On 02 Aug, 2020
Editor invited
On 02 Aug, 2020
Version 1
Posted 28 May, 2020
No comments provided
Editorial decision: Major revision
On 28 Jun, 2020
Review #2 received
Received 27 Jun, 2020
Review #1 received
Received 24 Jun, 2020
Reviewer #2 agreed
On 13 Jun, 2020
Reviewer #1 agreed
On 10 Jun, 2020
Reviewers invited
Invitations sent on 09 Jun, 2020
Editor invited
On 02 Jun, 2020
Editor assigned
On 01 Jun, 2020
Submission checks complete
On 26 May, 2020
First submitted
On 22 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Molecular Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Molecular Medicine.
Background The expression of SIN3A is closely correlated with EA treatment efficacy of SIA, but its underlying mechanisms remain to be further explored.
Methods Quantitative real-time PCR was performed to analyze the expression of candidate miRNAs and SIN3A mRNA in a rat model of SIA. Western blot was carried out to evaluate the differential expression of SIN3A proteins under distinct circumstances. Luciferase assay was used to explore the inhibitory role of certain miRNAs in SIN3A expression. NOR test was performed to assess the memorial ability of SIA rats undergoing EA treatment. Immunohistochemistry was carried out to evaluate the expression of SIN3A in the hippocampus of SIA rats.
Results Rno-miR-183-5p, rno-miR-34c-3p and rno-miR-210-3p were significantly up-regulated in SIA rats treated with EA. In addition, rno-miR-183-5p and rno-miR-210-3p exerted an inhibitory effect on SIN3A expression. EA treatment of SIA rats effectively restored the normal expression of rno-miR-183-5p, rno-miR-210-3p and SIN3A. The NOR test also confirmed the effect of EA treatment on the improvement of memory in SIA rats.
Conclusion In this study, an animal model of SIA was treated with EA to investigate its therapeutic effect. Moreover, our work presented solid evidence on the regulatory pathway of miR-183/SIN3A and miR-210/SIN3A in the pathogenesis of SIA.
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Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
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