See the published version of this article at BMC Musculoskeletal Disorders.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Parecoxib alleviates the inflammatory effect of leukocyte-rich platelet-rich plasma in normal rabbit tendons
Ning Wang
Chinese PLA General Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3337-137X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Musculoskeletal Disorders.
Background: Platelet-rich plasma (PRP) is widely used to treat tendon injuries. Its therapeutic effect varies depending on the different cell components, and white blood cells (WBCs) may play an important role in this phenomenon. The purpose of this study was to evaluate how PRP with different concentrations of WBCs affect normal rabbit tendon and assess whether non-steroidal anti-inflammatory drugs (NSAIDs) can suppress the catabolic effects of WBCs.
Methods: Sixteen adult New Zealand White rabbits were used. Blood samples were collected from each rabbit, and PRP was extracted following two different protocols to obtain leukocyte-poor PRP (LP-PRP) and leukocyte-rich PRP (LR-PRP). LP-PRP or LR-PRP was injected into the patellar tendon of each rabbit, while normal saline (NS) was injected as control. In LR-PRP + NSAID group, Parecoxib was administered after LR-PRP injection. For each group, 2 rabbits were euthanatized at day 5 and 14. The patellar tendons were collected and stained with hematoxylin and eosin. A semi-quantitative approach was used to assess the inflammatory response and tendon destruction based on the evaluation of the WBCs, vascularization, fiber structure, and fibrosis.
Results: The LR-PRP group exhibited a higher total tendon score than the LP-PRP group at day 5 after PRP injection, but there was no significant difference between the two groups at day 14. For the NSAID group, the tendon score was lower than that of the LR-PRP group both at day 5 and 14.
Conclusion: LR-PRP can promote a higher inflammatory response than LP-PRP in the normal rabbit patellar tendon, and this effect can be suppressed by NSAIDs.
Keywords
Platelet-rich plasma, Parecoxib, Nonsteroidal anti-inflammatory drugs, Tendinopathy
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 3
Posted 24 Nov, 2020
No comments provided
Editorial decision: Minor revision
On 28 Oct, 2020
Review #2 received
Received 26 Oct, 2020
Reviewer #2 agreed
On 05 Oct, 2020
Reviewers invited
Invitations sent on 04 Oct, 2020
Reviewer #1 agreed
On 04 Oct, 2020
Review #1 received
Received 04 Oct, 2020
Editor assigned
On 24 Sep, 2020
Editor invited
On 23 Sep, 2020
Submission checks complete
On 23 Sep, 2020
No comments provided
Editorial decision: Major revision
On 05 Sep, 2020
Review #2 received
Received 30 Aug, 2020
Reviewer #2 agreed
On 10 Aug, 2020
Review #1 received
Received 15 Jul, 2020
Reviewer #1 agreed
On 22 Jun, 2020
Reviewers invited
Invitations sent on 11 Jun, 2020
Editor assigned
On 26 May, 2020
First submitted
On 25 May, 2020
Submission checks complete
On 25 May, 2020
Editor invited
On 25 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Orthopedics
Learn more about our company.
This preprint is under consideration at BMC Musculoskeletal Disorders. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Parecoxib alleviates the inflammatory effect of leukocyte-rich platelet-rich plasma in normal rabbit tendons
Ning Wang
Chinese PLA General Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3337-137X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 3
Posted 24 Nov, 2020
No comments provided
Editorial decision: Minor revision
On 28 Oct, 2020
Review #2 received
Received 26 Oct, 2020
Reviewer #2 agreed
On 05 Oct, 2020
Reviewers invited
Invitations sent on 04 Oct, 2020
Reviewer #1 agreed
On 04 Oct, 2020
Review #1 received
Received 04 Oct, 2020
Editor assigned
On 24 Sep, 2020
Editor invited
On 23 Sep, 2020
Submission checks complete
On 23 Sep, 2020
No comments provided
Editorial decision: Major revision
On 05 Sep, 2020
Review #2 received
Received 30 Aug, 2020
Reviewer #2 agreed
On 10 Aug, 2020
Review #1 received
Received 15 Jul, 2020
Reviewer #1 agreed
On 22 Jun, 2020
Reviewers invited
Invitations sent on 11 Jun, 2020
Editor assigned
On 26 May, 2020
First submitted
On 25 May, 2020
Submission checks complete
On 25 May, 2020
Editor invited
On 25 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Orthopedics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Musculoskeletal Disorders.
Background: Platelet-rich plasma (PRP) is widely used to treat tendon injuries. Its therapeutic effect varies depending on the different cell components, and white blood cells (WBCs) may play an important role in this phenomenon. The purpose of this study was to evaluate how PRP with different concentrations of WBCs affect normal rabbit tendon and assess whether non-steroidal anti-inflammatory drugs (NSAIDs) can suppress the catabolic effects of WBCs.
Methods: Sixteen adult New Zealand White rabbits were used. Blood samples were collected from each rabbit, and PRP was extracted following two different protocols to obtain leukocyte-poor PRP (LP-PRP) and leukocyte-rich PRP (LR-PRP). LP-PRP or LR-PRP was injected into the patellar tendon of each rabbit, while normal saline (NS) was injected as control. In LR-PRP + NSAID group, Parecoxib was administered after LR-PRP injection. For each group, 2 rabbits were euthanatized at day 5 and 14. The patellar tendons were collected and stained with hematoxylin and eosin. A semi-quantitative approach was used to assess the inflammatory response and tendon destruction based on the evaluation of the WBCs, vascularization, fiber structure, and fibrosis.
Results: The LR-PRP group exhibited a higher total tendon score than the LP-PRP group at day 5 after PRP injection, but there was no significant difference between the two groups at day 14. For the NSAID group, the tendon score was lower than that of the LR-PRP group both at day 5 and 14.
Conclusion: LR-PRP can promote a higher inflammatory response than LP-PRP in the normal rabbit patellar tendon, and this effect can be suppressed by NSAIDs.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3