Background: Cystic and alveolar echinococcosis caused by the tapeworms Echinococcus granulosus and E. multilocularis, respectively, are important zoonotic diseases. Protease inhibitors are crucial for the survival of both Echinococcus spp. Kunitz-type inhibitors play a regulatory role in the control of protease activity. In this study,we identified all the Kunitz-type protease inhibitors present in the genomes of these two tapeworms and analyzed the gene sequences using computational, structural bioinformatics and phylogenetic approaches to evaluate the evolutionary relationships of these genes.
Results: A total of 19 genes from E. multilocularis and 23 genes from E. granulosus contained single or multiple Kunitz-domains. A neighbor-joining phylogenetic tree indicated that the E. granulosus and E. multilocularis Kunitz domain peptides were divided into three branches containing 9 clusters. Based on available transcriptome data, we analyzed the expression of these Kunitz-domain protease inhibitors in four major developmental stages of E. granulosus and found they were differentially expressed.
Conclusion: We identified 19 and 23 Kunitz protease inhibitors in E. multilocularis and E. granulosus respectively; the majority of these genes were expressed in one or four stages of E. granulosus with some being highly expressed in adult worms indicating that these genes likely play different roles in the different developmental stages.
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No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
Additional file 1 Table S1 Physiological and biological characteristics of each of E. granulosus and E. multilocularis KDPIs
Additional file 2 Table S2 The cellular localization of the E. multilocularis and E. granulosus KDPIs
Additional file 3 Figure S1 Structure and amino acid composition of a Kunitz-domain peptide. Three disulfide bridges are formed by 6 cysteines. Conserved cysteine residues are marked in bold font.
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Posted 22 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
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On 30 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
Invitations sent on 30 Mar, 2021
On 25 Mar, 2021
On 19 Mar, 2021
On 19 Mar, 2021
On 11 Mar, 2021
Posted 22 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
Invitations sent on 30 Mar, 2021
On 25 Mar, 2021
On 19 Mar, 2021
On 19 Mar, 2021
On 11 Mar, 2021
Background: Cystic and alveolar echinococcosis caused by the tapeworms Echinococcus granulosus and E. multilocularis, respectively, are important zoonotic diseases. Protease inhibitors are crucial for the survival of both Echinococcus spp. Kunitz-type inhibitors play a regulatory role in the control of protease activity. In this study,we identified all the Kunitz-type protease inhibitors present in the genomes of these two tapeworms and analyzed the gene sequences using computational, structural bioinformatics and phylogenetic approaches to evaluate the evolutionary relationships of these genes.
Results: A total of 19 genes from E. multilocularis and 23 genes from E. granulosus contained single or multiple Kunitz-domains. A neighbor-joining phylogenetic tree indicated that the E. granulosus and E. multilocularis Kunitz domain peptides were divided into three branches containing 9 clusters. Based on available transcriptome data, we analyzed the expression of these Kunitz-domain protease inhibitors in four major developmental stages of E. granulosus and found they were differentially expressed.
Conclusion: We identified 19 and 23 Kunitz protease inhibitors in E. multilocularis and E. granulosus respectively; the majority of these genes were expressed in one or four stages of E. granulosus with some being highly expressed in adult worms indicating that these genes likely play different roles in the different developmental stages.
Figure 1
Figure 2
Figure 3
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