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Research article
Genetic Regulatory Networks for Salt-Alkali Stress in Gossypium hirsutum With Differing Morphological Characteristics
Fang Liu
ISTITUTE OF COTTON RESEARCH
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6213-9572
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomics.
Background
Cotton grows in altering environments that are often unfavorable or stressful for its growth and development. Consequently, the plant must cope with abiotic stresses such as soil salinity, drought, and excessive temperatures. Alkali-salt stress response remains a cumbersome biological process and is regulated via a multifaceted transcriptional regulatory network in cotton
Results
To discover the molecular mechanisms of alkali-salt stress response in cotton, a comprehensive transcriptome analysis was carried out after alkali-salt stress treatment in three accessions of Gossypium hirsutum with contrasting phenotype. Expression level analysis proved that alkali-salt stress response presented significant stage-specific and tissue-specific. GO enrichment analysis typically suggested that signal transduction process involved in salt-alkali stress response at SS3 and SS12 stages in leaf; carbohydrate metabolic process and oxidation-reduction process involved in SS48 stages in leaf; the oxidation-reduction process involved at all three phases in the root. The Co-expression analysis suggested a potential GhSOS3/GhCBL10-SOS2 network was involved in salt-alkali stress response. Furthermore, Salt-alkali sensitivity was increased in GhSOS3 and GhCBL10 Virus-induced Gene Silencing (VIGS) plants.
Conclusion
The findings may facilitate to elucidate the underlying mechanisms of alkali-salt stress response and provide an available resource to scrutinize the role of candidate genes and signaling pathway governing alkali-salt stress response Keywords: Alkali-Salt Stress; RNA-Seq; Gene Co-Expression; Gossypium Hirsutum Races; WGCNA
Keywords
Alkali-Salt Stress, RNA-Seq, Gene Co-Expression, Gossypium Hirsutum Races, WGCNA
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CURRENT STATUS: Published
Version 3
Posted 18 Dec, 2019
Published
On 06 Jan, 2020
No community comments so far
Submission checks complete
On 06 Dec, 2019
Editorial decision: Accept
On 06 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 21 Nov, 2019
Review #2 received
Received 14 Nov, 2019
Review #1 received
Received 29 Oct, 2019
Reviewer #2 agreed
On 17 Oct, 2019
Reviewer #1 agreed
On 12 Oct, 2019
Editor assigned
On 11 Oct, 2019
Reviewers invited
Invitations sent on 11 Oct, 2019
Submission checks complete
On 10 Oct, 2019
Editor invited
On 10 Oct, 2019
No comments provided
Editorial decision: Minor revision
On 01 Oct, 2019
Review #1 received
Received 22 Aug, 2019
Review #2 received
Received 22 Aug, 2019
Reviewer #2 agreed
On 04 Aug, 2019
Reviewer #1 agreed
On 03 Aug, 2019
Reviewers invited
Invitations sent on 02 Aug, 2019
Submission checks complete
On 30 Jul, 2019
Editor invited
On 30 Jul, 2019
Editor assigned
On 29 Jul, 2019
First submitted
On 26 Jul, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
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This preprint is under consideration at BMC Genomics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Genetic Regulatory Networks for Salt-Alkali Stress in Gossypium hirsutum With Differing Morphological Characteristics
Fang Liu
ISTITUTE OF COTTON RESEARCH
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6213-9572
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 3
Posted 18 Dec, 2019
Published
On 06 Jan, 2020
No community comments so far
Submission checks complete
On 06 Dec, 2019
Editorial decision: Accept
On 06 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 21 Nov, 2019
Review #2 received
Received 14 Nov, 2019
Review #1 received
Received 29 Oct, 2019
Reviewer #2 agreed
On 17 Oct, 2019
Reviewer #1 agreed
On 12 Oct, 2019
Editor assigned
On 11 Oct, 2019
Reviewers invited
Invitations sent on 11 Oct, 2019
Submission checks complete
On 10 Oct, 2019
Editor invited
On 10 Oct, 2019
No comments provided
Editorial decision: Minor revision
On 01 Oct, 2019
Review #1 received
Received 22 Aug, 2019
Review #2 received
Received 22 Aug, 2019
Reviewer #2 agreed
On 04 Aug, 2019
Reviewer #1 agreed
On 03 Aug, 2019
Reviewers invited
Invitations sent on 02 Aug, 2019
Submission checks complete
On 30 Jul, 2019
Editor invited
On 30 Jul, 2019
Editor assigned
On 29 Jul, 2019
First submitted
On 26 Jul, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomics.
Background
Cotton grows in altering environments that are often unfavorable or stressful for its growth and development. Consequently, the plant must cope with abiotic stresses such as soil salinity, drought, and excessive temperatures. Alkali-salt stress response remains a cumbersome biological process and is regulated via a multifaceted transcriptional regulatory network in cotton
Results
To discover the molecular mechanisms of alkali-salt stress response in cotton, a comprehensive transcriptome analysis was carried out after alkali-salt stress treatment in three accessions of Gossypium hirsutum with contrasting phenotype. Expression level analysis proved that alkali-salt stress response presented significant stage-specific and tissue-specific. GO enrichment analysis typically suggested that signal transduction process involved in salt-alkali stress response at SS3 and SS12 stages in leaf; carbohydrate metabolic process and oxidation-reduction process involved in SS48 stages in leaf; the oxidation-reduction process involved at all three phases in the root. The Co-expression analysis suggested a potential GhSOS3/GhCBL10-SOS2 network was involved in salt-alkali stress response. Furthermore, Salt-alkali sensitivity was increased in GhSOS3 and GhCBL10 Virus-induced Gene Silencing (VIGS) plants.
Conclusion
The findings may facilitate to elucidate the underlying mechanisms of alkali-salt stress response and provide an available resource to scrutinize the role of candidate genes and signaling pathway governing alkali-salt stress response Keywords: Alkali-Salt Stress; RNA-Seq; Gene Co-Expression; Gossypium Hirsutum Races; WGCNA
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8