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Research Article
Eliška Konopáčová
University of Pardubice
Corresponding Author
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Epilithon contributes to phosphorus (P) cycling in lakes, but its P uptake traits have been rarely studied. We measured the chemical composition of epilithon and its inorganic P uptake kinetics using isotope 33P in three deep oligo- to mesotrophic post-mining lakes in April, July, and October 2019. Over the sampling period, epilithon biomass doubled, while the P content in biomass dropped to 60% of the April values. High epilithic C:P molar ratios (677 on average) suggested strong P deficiency in all investigated lakes. Regarding the kinetic parameters of phosphorus uptake, maximum uptake velocity (Vmax, seasonal range 0.9–101 mg P g OM–1 h–1) decreased by an order of magnitude from April to October, while half-saturation constant (KS, 1.6–103 mg P L–1) did not show any consistent temporal trend. We found a general decrease in the specific P uptake affinity (0.1–2.2 L g OM–1 h–1) and the estimated in-situ P uptake (0.04–2.3 µg P g OM–1 h–1) of epilithon over the season, which might have resulted from changes in epilithon community composition, a decreasing ratio of living biomass to extracellular polymers and detritus, rapid internal recycling, and/or thickening of the epilithic biofilm. Additionally, we observed a phenomenon of reversible abiotic P adsorption on epilithon.
Keywords
mesotrophic post-mining lakes, epilithon, phosphorus (P) cycling
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Eliška Konopáčová
University of Pardubice
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 18 Mar, 2021
No community comments so far
Reviewer #1 agreed
On 05 Apr, 2021
Reviewer #2 agreed
On 05 Apr, 2021
Reviewer #3 agreed
On 05 Apr, 2021
Reviewers invited
Invitations sent on 24 Mar, 2021
Editor assigned
On 17 Mar, 2021
Editor invited
On 17 Mar, 2021
Submission checks complete
On 17 Mar, 2021
First submitted
On 12 Mar, 2021
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Epilithon contributes to phosphorus (P) cycling in lakes, but its P uptake traits have been rarely studied. We measured the chemical composition of epilithon and its inorganic P uptake kinetics using isotope 33P in three deep oligo- to mesotrophic post-mining lakes in April, July, and October 2019. Over the sampling period, epilithon biomass doubled, while the P content in biomass dropped to 60% of the April values. High epilithic C:P molar ratios (677 on average) suggested strong P deficiency in all investigated lakes. Regarding the kinetic parameters of phosphorus uptake, maximum uptake velocity (Vmax, seasonal range 0.9–101 mg P g OM–1 h–1) decreased by an order of magnitude from April to October, while half-saturation constant (KS, 1.6–103 mg P L–1) did not show any consistent temporal trend. We found a general decrease in the specific P uptake affinity (0.1–2.2 L g OM–1 h–1) and the estimated in-situ P uptake (0.04–2.3 µg P g OM–1 h–1) of epilithon over the season, which might have resulted from changes in epilithon community composition, a decreasing ratio of living biomass to extracellular polymers and detritus, rapid internal recycling, and/or thickening of the epilithic biofilm. Additionally, we observed a phenomenon of reversible abiotic P adsorption on epilithon.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This preprint is available for download as a PDF.
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