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Research article
Peimin Wang
Affiliated Hospital of Nanjing University of Chinese
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3675-7326
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: To clarify the therapeutic potential of imperatorin (IMP) in knee osteoarthritis (KOA).
Methods: Thirty 3-month-old SD male rats were randomly divided into Normal group, monosodium iodoacetate (MIA) group and MIA+IMP group. Their synovial tissues were subjected to histopathological analysis. Primary synovial fibroblasts obtained from additional normal rats were treated by lipopolysaccharide (LPS) and then IMP. The mRNA and protein expressions of factors related to synovitis and synovial fibrosis were detected by qRT-PCR and Western blotting, respectively. The levels of inflammatory factors IL-1β and IL-18 were measured by ELISA.
Results: IMP reduced HIF-1α, NLRP3 inflammasome expression and IL-1β, IL-18 production in synovial fibroblasts induced by LPS. IMP also down-regulated synovial fibrosis markers. In vitro study revealed that MIA-induced synovitis and synovial fibrosis were relieved by IMP.
Conclusion: IMP exerts anti-inflammatory effects associated with synovitis and synovial fibrosis. It reduces the production of pro-inflammatory mediators and cytokines and inhibits TGF-β1, TIMP-1 and VEGF expressions that promote synovial fibrosis.
Keywords
imperatorin, knee osteoarthritis, synovial fibrosis, hypoxia, synovitis, NLRP3 inflammasome
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Peimin Wang
Affiliated Hospital of Nanjing University of Chinese
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3675-7326
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 23 Mar, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: To clarify the therapeutic potential of imperatorin (IMP) in knee osteoarthritis (KOA).
Methods: Thirty 3-month-old SD male rats were randomly divided into Normal group, monosodium iodoacetate (MIA) group and MIA+IMP group. Their synovial tissues were subjected to histopathological analysis. Primary synovial fibroblasts obtained from additional normal rats were treated by lipopolysaccharide (LPS) and then IMP. The mRNA and protein expressions of factors related to synovitis and synovial fibrosis were detected by qRT-PCR and Western blotting, respectively. The levels of inflammatory factors IL-1β and IL-18 were measured by ELISA.
Results: IMP reduced HIF-1α, NLRP3 inflammasome expression and IL-1β, IL-18 production in synovial fibroblasts induced by LPS. IMP also down-regulated synovial fibrosis markers. In vitro study revealed that MIA-induced synovitis and synovial fibrosis were relieved by IMP.
Conclusion: IMP exerts anti-inflammatory effects associated with synovitis and synovial fibrosis. It reduces the production of pro-inflammatory mediators and cytokines and inhibits TGF-β1, TIMP-1 and VEGF expressions that promote synovial fibrosis.
Figure 1
Figure 2
Figure 3
Figure 4
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