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Research Article
Sally Shalaby
Zagazig University- Faculty of Medicine
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2868-020X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Because of low sensitivity and specificity of the currently available urine markers for bladder cancer (BC) detection and painful cystoscopy procedure, our study was aimed to evaluate expression of urinary exosomal microRNA (miR)-96 and miR-183 as probable noninvasive and accurate biomarkers for the diagnosis and follow up of BC.
Methods and results: Using quantitative real-time polymerase chain reaction; Expression of exosomal miR-96 and miR- 183 in the urine samples of 51 patients with BC, 21 patients with benign urinary bladder lesions and in 24 normal individuals as control group. Our study results showed higher expressions of both miR-96 and miR-183 in urine of BC patients in comparison with control group (P <0.001 for each). Analysis of receiver-operating characteristic curve demonstrated that each microRNA had good sensitivity and specificity for differentiating BC patients from non-BC patients (miR-96; 80.4% and 91.8%; and miR-183, 78.4% and 81.6% respectively) compared to cytology (37.3% and 100%). In addition, it was obvious that the sensitivity and specificity of combined miR-96 and miR-183 for the diagnosis of BC reached 88.2%% and 87.8%, respectively, which were higher than each one alone. We also found that expression of miR-96 and miR-183 with lymph node invasion, advancing grade, and pathological stage was significantly increased. After surgery, collected urine samples showed significantly lower expression of microRNA (miR-96: P < 0.001; and miR-183, P = 0.002).
Conclusion: In conclusion, miR-96 and miR-183 in urine are promising diagnostic tumor markers for BC; particularly, when they combined with each other or with urinary cytology.
Keywords
Bladder cancer, miRNA-96; miRNA-183; biochemical marker.
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This preprint is under consideration at Molecular Biology Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Sally Shalaby
Zagazig University- Faculty of Medicine
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2868-020X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 24 Mar, 2021
No community comments so far
Editorial decision: Minor Revisions Needed
On 19 Apr, 2021
Review #? received
Received 21 Mar, 2021
Reviewers invited
Invitations sent on 21 Mar, 2021
Editor assigned
On 12 Mar, 2021
First submitted
On 12 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Molecular Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Because of low sensitivity and specificity of the currently available urine markers for bladder cancer (BC) detection and painful cystoscopy procedure, our study was aimed to evaluate expression of urinary exosomal microRNA (miR)-96 and miR-183 as probable noninvasive and accurate biomarkers for the diagnosis and follow up of BC.
Methods and results: Using quantitative real-time polymerase chain reaction; Expression of exosomal miR-96 and miR- 183 in the urine samples of 51 patients with BC, 21 patients with benign urinary bladder lesions and in 24 normal individuals as control group. Our study results showed higher expressions of both miR-96 and miR-183 in urine of BC patients in comparison with control group (P <0.001 for each). Analysis of receiver-operating characteristic curve demonstrated that each microRNA had good sensitivity and specificity for differentiating BC patients from non-BC patients (miR-96; 80.4% and 91.8%; and miR-183, 78.4% and 81.6% respectively) compared to cytology (37.3% and 100%). In addition, it was obvious that the sensitivity and specificity of combined miR-96 and miR-183 for the diagnosis of BC reached 88.2%% and 87.8%, respectively, which were higher than each one alone. We also found that expression of miR-96 and miR-183 with lymph node invasion, advancing grade, and pathological stage was significantly increased. After surgery, collected urine samples showed significantly lower expression of microRNA (miR-96: P < 0.001; and miR-183, P = 0.002).
Conclusion: In conclusion, miR-96 and miR-183 in urine are promising diagnostic tumor markers for BC; particularly, when they combined with each other or with urinary cytology.
Figure 1
Figure 2
Figure 3
This preprint is available for download as a PDF.
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