Background: Non-communicable diseases are the leading cause of death and the main issue in modern and future public health worldwide. Non-communicable diseases are amenable to health promotion, prevention and medical interventions. Improved social conditions, quality of diet, body weight, smoking cessation, and increased physical activity can greatly reduce the burden of NCDs. Methods: This study aims to assess the attitude and practice of medical students towards the role of physical activity in non- communicable diseases prevention in Nile University as perceived by students. An Observational-based analytical, cross-sectional study design. Data analysis was done using the SPSS software. Result: The study included 81 males(43.1%) and 107 females(56.9%). Out of total number of students , 51% do not engage in physical activities and 49% participate in planned physical activities. The frequency of participation in physical activities are; Students who engage in physical activities daily (14, 7.4%) and weekly (37, 19.7%), while 40 students (21.3%) engage in physical activity monthly. Students that participate in planned physical activities for health benefits were 86(45.7%), and 4 students (2.1%) practice physical activities for social reasons, while a single student practices due to doctor’s advice 1 (5%). The majority of students had good background knowledge (83%) and most provde advice to their friends (38.3%) and family (51.1&). Conclusion: Maority of the students had an acceptable background knowledge about the role of physical activities in prevention of major NCDs. However, they had low frequency of participation in planned physical activities despite having positive advocating attitude.