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Predictive significance of arachidonate 15-lipoxygenase for eosinophilic chronic rhinosinusitis with nasal polyps
Luo Zhang
Beijing Tongren Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0910-9884
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View the published version at Allergy, Asthma & Clinical Immunology.
Background: Eosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP) exhibits a poorer outcome compared with non-eosinophilic chronic rhinosinusitis with nasal polyps (nonECRSwNP), so it is significant to identify effective markers to differentiate ECRSwNP in guiding the treatment strategies of these patients. Although arachidonate 15-lipoxygenase (ALOX15) is positioned as a marker of eosinophilic inflammation, its study in differentiating ECRSwNP has not been reported. The aim of this study is to assess the potential of ALOX15 in distinguishing and predicting ECRSwNP.
Methods: Forty-eight patients with chronic rhinosinusitis with nasal polyps (CRSwNP), including 30 ECRSwNP and 18 nonECRSwNP patients, were enrolled. ALOX15 mRNA level was determined in polyps by real-time polymerase chain reaction (RT-PCR). The patients’ baseline characteristics were evaluated and analyzed for correlations with ALOX15. Receiver operating characteristic (ROC) curve was used to assess the predictive significance of the potential predictors for ECRSwNP.
Results: ALOX15 mRNA level was significantly higher in ECRSwNP patients than in nonECRSwNP patients (P < 0.001). ALOX15 mRNA was significantly correlated with tissue and blood eosinophil percentages (r = 0.565, P < 0.001 and r = 0.395, P = 0.006), olfaction scores (r = 0.400, P = 0.005), total visual analogue scale (VAS) symptom scores (r = 0.383, P = 0.007), ethmoid/maxillary sinus (E/M) ratio (r = 0.463, P = 0.001), and endoscopy scores (r = 0.409, P = 0.004). Logistic regression analysis showed ALOX15 mRNA level and percentage of blood eosinophils to be predictive factors for ECRSwNP (P = 0.004 and P = 0.036, respectively). ROC curve indicated ALOX15 to have high predictive accuracy for ECRSwNP (area under the curve (AUC) = 0.909), which was further improved by combination of ALOX15 with percentage of blood eosinophils (AUC = 0.933).
Conclusions: The relative ALOX15 mRNA level alone or in combination with blood eosinophils might be a reliable biomarker for predicting a diagnosis of ECRSwNP.
Keywords
Arachidonate 15-lipoxygenase, Biomarker, Chronic rhinosinusitis with nasal polyps, Eosinophils, Predictive value, Receiver operating characteristic curve
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CURRENT STATUS: Published
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Posted 20 Aug, 2020
Published
On 16 Sep, 2020
No community comments so far
Editorial decision: Accept
On 02 Sep, 2020
Reviewer #2 agreed
On 01 Sep, 2020
Review #2 received
Received 01 Sep, 2020
Review #1 received
Received 30 Aug, 2020
Reviewer #1 agreed
On 20 Aug, 2020
Editor assigned
On 19 Aug, 2020
Reviewers invited
Invitations sent on 19 Aug, 2020
Submission checks complete
On 18 Aug, 2020
Editor invited
On 18 Aug, 2020
No comments provided
Review #2 received
Received 26 Jun, 2020
Editorial decision: Major revision
On 26 Jun, 2020
Review #1 received
Received 08 Jun, 2020
Reviewer #2 agreed
On 07 Jun, 2020
Reviewers invited
Invitations sent on 01 Jun, 2020
Reviewer #1 agreed
On 01 Jun, 2020
First submitted
On 29 May, 2020
Editor assigned
On 29 May, 2020
Submission checks complete
On 28 May, 2020
Editor invited
On 28 May, 2020
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Subject Areas
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This preprint is under consideration at Allergy, Asthma & Clinical Immunology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Predictive significance of arachidonate 15-lipoxygenase for eosinophilic chronic rhinosinusitis with nasal polyps
Luo Zhang
Beijing Tongren Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0910-9884
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 20 Aug, 2020
Published
On 16 Sep, 2020
No community comments so far
Editorial decision: Accept
On 02 Sep, 2020
Reviewer #2 agreed
On 01 Sep, 2020
Review #2 received
Received 01 Sep, 2020
Review #1 received
Received 30 Aug, 2020
Reviewer #1 agreed
On 20 Aug, 2020
Editor assigned
On 19 Aug, 2020
Reviewers invited
Invitations sent on 19 Aug, 2020
Submission checks complete
On 18 Aug, 2020
Editor invited
On 18 Aug, 2020
No comments provided
Review #2 received
Received 26 Jun, 2020
Editorial decision: Major revision
On 26 Jun, 2020
Review #1 received
Received 08 Jun, 2020
Reviewer #2 agreed
On 07 Jun, 2020
Reviewers invited
Invitations sent on 01 Jun, 2020
Reviewer #1 agreed
On 01 Jun, 2020
First submitted
On 29 May, 2020
Editor assigned
On 29 May, 2020
Submission checks complete
On 28 May, 2020
Editor invited
On 28 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Allergy & Immune Disorders
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Allergy, Asthma & Clinical Immunology.
Background: Eosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP) exhibits a poorer outcome compared with non-eosinophilic chronic rhinosinusitis with nasal polyps (nonECRSwNP), so it is significant to identify effective markers to differentiate ECRSwNP in guiding the treatment strategies of these patients. Although arachidonate 15-lipoxygenase (ALOX15) is positioned as a marker of eosinophilic inflammation, its study in differentiating ECRSwNP has not been reported. The aim of this study is to assess the potential of ALOX15 in distinguishing and predicting ECRSwNP.
Methods: Forty-eight patients with chronic rhinosinusitis with nasal polyps (CRSwNP), including 30 ECRSwNP and 18 nonECRSwNP patients, were enrolled. ALOX15 mRNA level was determined in polyps by real-time polymerase chain reaction (RT-PCR). The patients’ baseline characteristics were evaluated and analyzed for correlations with ALOX15. Receiver operating characteristic (ROC) curve was used to assess the predictive significance of the potential predictors for ECRSwNP.
Results: ALOX15 mRNA level was significantly higher in ECRSwNP patients than in nonECRSwNP patients (P < 0.001). ALOX15 mRNA was significantly correlated with tissue and blood eosinophil percentages (r = 0.565, P < 0.001 and r = 0.395, P = 0.006), olfaction scores (r = 0.400, P = 0.005), total visual analogue scale (VAS) symptom scores (r = 0.383, P = 0.007), ethmoid/maxillary sinus (E/M) ratio (r = 0.463, P = 0.001), and endoscopy scores (r = 0.409, P = 0.004). Logistic regression analysis showed ALOX15 mRNA level and percentage of blood eosinophils to be predictive factors for ECRSwNP (P = 0.004 and P = 0.036, respectively). ROC curve indicated ALOX15 to have high predictive accuracy for ECRSwNP (area under the curve (AUC) = 0.909), which was further improved by combination of ALOX15 with percentage of blood eosinophils (AUC = 0.933).
Conclusions: The relative ALOX15 mRNA level alone or in combination with blood eosinophils might be a reliable biomarker for predicting a diagnosis of ECRSwNP.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6