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Mohammad Tahseen Albataineh
University of Sharjah College of Medicine
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8855-5320
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Background: Heavy tobacco smoking, a hallmark feature of lung cancer, is drastically predominant in Middle Eastern populations. The precise links between nicotine dependence and the functional contribution of the oral microbiota remain unknown in these populations.
Methods: We evaluated the composition and functional capabilities of oral microbiota with relation to cigarette smoking in 105 adults through shotgun metagenomics using buccal swabs.
Results: The oral microbiota composition in our study subjects was dominated by the phyla Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes, in addition to the genera Prevotella and Veillonella, similar to previously described westernized cohorts. Furthermore, the smoker's oral microbiota represented a significant abundance of Veillonella dispar, Leptotrichia spp., and Prevotella pleuritidis when compared to non-smokers. Within the smoking groups, differential relative abundance testing unveiled relative abundance of Streptobacillus hongkongensis, Fusobacterium massiliense, Prevotella bivia in high nicotine dependent compared to low nicotine dependent profiles based on Fagerström Test for Nicotine Dependence. Functional profiling showed marked differences between smokers and non-smokers. Smokers exhibited an enrichment of Tricarballylate utilization and Lactate racemization when compared to the non-smokers. According to their nicotine dependence, enrichment of Xanthosine utilization, p-Aminobenzoyl-Glutamate utilization, and multidrug efflux pump in Campylobacter jejuni biosynthesis modules were detected in the high nicotine dependent group.
Conclusions: These compositional and functional differences may provide critical insight on how variations in the oral microbiota could predispose to respiratory illnesses and smoke cessation relapse in cigarette smokers. In particular, the observed enrichment of Fusobacterium and Prevotella in the oral microbiota possibly suggests an intriguing linkage to gut and lung cancers.
Keywords
Fagerström test, Nicotine dependence, Oral microbiota, Shotgun metagenomics
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CURRENT STATUS: Published
View the published article at Journal of Translational Medicine.
Version 3
Posted 27 Oct, 2020
No community comments so far
Editor assigned
On 19 Oct, 2020
Editorial decision: Accept
On 19 Oct, 2020
Submission checks complete
On 18 Oct, 2020
Editor invited
On 18 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 13 Oct, 2020
Review #2 received
Received 01 Oct, 2020
Reviewer #3 agreed
On 28 Sep, 2020
Review #3 received
Received 28 Sep, 2020
Reviewer #2 agreed
On 27 Sep, 2020
Review #1 received
Received 25 Sep, 2020
Reviewers invited
Invitations sent on 24 Sep, 2020
Reviewer #1 agreed
On 24 Sep, 2020
Editor assigned
On 17 Sep, 2020
Submission checks complete
On 16 Sep, 2020
Editor invited
On 16 Sep, 2020
No comments provided
Editorial decision: Major revision
On 14 Aug, 2020
Review #3 received
Received 26 Jul, 2020
Review #4 received
Received 26 Jul, 2020
Review #5 received
Received 24 Jul, 2020
Review #1 received
Received 14 Jul, 2020
Reviewer #5 agreed
On 12 Jul, 2020
Reviewer #4 agreed
On 10 Jul, 2020
Reviewer #2 agreed
On 07 Jul, 2020
Reviewer #3 agreed
On 07 Jul, 2020
Review #2 received
Received 07 Jul, 2020
Reviewer #1 agreed
On 06 Jul, 2020
Reviewers invited
Invitations sent on 03 Jul, 2020
Editor assigned
On 12 Jun, 2020
Editor invited
On 11 Jun, 2020
Submission checks complete
On 04 Jun, 2020
First submitted
On 02 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Translational Medicine
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Journal of Translational Medicine.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Mohammad Tahseen Albataineh
University of Sharjah College of Medicine
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8855-5320
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of Translational Medicine.
Version 3
Posted 27 Oct, 2020
No community comments so far
Editor assigned
On 19 Oct, 2020
Editorial decision: Accept
On 19 Oct, 2020
Submission checks complete
On 18 Oct, 2020
Editor invited
On 18 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 13 Oct, 2020
Review #2 received
Received 01 Oct, 2020
Reviewer #3 agreed
On 28 Sep, 2020
Review #3 received
Received 28 Sep, 2020
Reviewer #2 agreed
On 27 Sep, 2020
Review #1 received
Received 25 Sep, 2020
Reviewers invited
Invitations sent on 24 Sep, 2020
Reviewer #1 agreed
On 24 Sep, 2020
Editor assigned
On 17 Sep, 2020
Submission checks complete
On 16 Sep, 2020
Editor invited
On 16 Sep, 2020
No comments provided
Editorial decision: Major revision
On 14 Aug, 2020
Review #3 received
Received 26 Jul, 2020
Review #4 received
Received 26 Jul, 2020
Review #5 received
Received 24 Jul, 2020
Review #1 received
Received 14 Jul, 2020
Reviewer #5 agreed
On 12 Jul, 2020
Reviewer #4 agreed
On 10 Jul, 2020
Reviewer #2 agreed
On 07 Jul, 2020
Reviewer #3 agreed
On 07 Jul, 2020
Review #2 received
Received 07 Jul, 2020
Reviewer #1 agreed
On 06 Jul, 2020
Reviewers invited
Invitations sent on 03 Jul, 2020
Editor assigned
On 12 Jun, 2020
Editor invited
On 11 Jun, 2020
Submission checks complete
On 04 Jun, 2020
First submitted
On 02 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Translational Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of Translational Medicine.
Background: Heavy tobacco smoking, a hallmark feature of lung cancer, is drastically predominant in Middle Eastern populations. The precise links between nicotine dependence and the functional contribution of the oral microbiota remain unknown in these populations.
Methods: We evaluated the composition and functional capabilities of oral microbiota with relation to cigarette smoking in 105 adults through shotgun metagenomics using buccal swabs.
Results: The oral microbiota composition in our study subjects was dominated by the phyla Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes, in addition to the genera Prevotella and Veillonella, similar to previously described westernized cohorts. Furthermore, the smoker's oral microbiota represented a significant abundance of Veillonella dispar, Leptotrichia spp., and Prevotella pleuritidis when compared to non-smokers. Within the smoking groups, differential relative abundance testing unveiled relative abundance of Streptobacillus hongkongensis, Fusobacterium massiliense, Prevotella bivia in high nicotine dependent compared to low nicotine dependent profiles based on Fagerström Test for Nicotine Dependence. Functional profiling showed marked differences between smokers and non-smokers. Smokers exhibited an enrichment of Tricarballylate utilization and Lactate racemization when compared to the non-smokers. According to their nicotine dependence, enrichment of Xanthosine utilization, p-Aminobenzoyl-Glutamate utilization, and multidrug efflux pump in Campylobacter jejuni biosynthesis modules were detected in the high nicotine dependent group.
Conclusions: These compositional and functional differences may provide critical insight on how variations in the oral microbiota could predispose to respiratory illnesses and smoke cessation relapse in cigarette smokers. In particular, the observed enrichment of Fusobacterium and Prevotella in the oral microbiota possibly suggests an intriguing linkage to gut and lung cancers.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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