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Research article
Yuling Bai
Wageningen University and Research Wageningen Plant Research
Corresponding Author
Xingfang Gu
Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers
Corresponding Author
Shengping Zhang
Chinese Academy of Agricultural Sciences
Corresponding Author
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Background
Powdery mildew (PM) is one of the most serious diseases in cucumber and causes huge yield loss. Multiple quantitative trait loci (QTLs) for PM resistance have been detected in previous studies, however, none of these studies used the approach of genome-wide association analysis (GWAS). In this study, a cucumber core germplasm (CG) consisting of 109 resequenced lines was evaluated for PM resistance in three seasons.
Results
twelve loci (pmG1.1, pmG2.1, pmG2.2, pmG3.1, pmG4.1, pmG4.2, pmG5.1, pmG5.2, pmG5.3, pmG5.4, pmG6.1 and pmG6.2) associated with PM resistance were detected on all chromosomes except for Chr.7 using GWAS. Among these loci, pmG2.1, pmG4.1 and pmG6.1 were the novel ones that have not been reported in previous studies. The loci of pmG2.1, pmG5.2, pmG5.3 showed stronger signal in three seasons, for which three candidate genes Csa2G022790, Csa5G484620 and Csa5G604330 were respectively predicted using pairwise LD correlation and qRT-PCR analysis. Further, Csa4G022270 in pmG4.1, Csa6G022350 and Csa6G022370 in pmG6.1 might be the candidate genes based on the annotation of homologous genes in Arabidopsis.
Conclusion
Twelve Loci related to powdery mildew resistance in cucumber were detected by genome-wide association analysis, three of which namely pmG2.1, pmG4.1 and pmG6.1 are novel. Three candidate genes within the genomic region of locus pmG2.1, pmG5.2 and pmG5.3 were identified, respectively. These results provide targets for gene cloning and genetic breeding of PM resistance in cucumber.
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This is a preprint. It has not completed peer review.
Research article
Yuling Bai
Wageningen University and Research Wageningen Plant Research
Corresponding Author
Xingfang Gu
Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers
Corresponding Author
Shengping Zhang
Chinese Academy of Agricultural Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 10 Jun, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Powdery mildew (PM) is one of the most serious diseases in cucumber and causes huge yield loss. Multiple quantitative trait loci (QTLs) for PM resistance have been detected in previous studies, however, none of these studies used the approach of genome-wide association analysis (GWAS). In this study, a cucumber core germplasm (CG) consisting of 109 resequenced lines was evaluated for PM resistance in three seasons.
Results
twelve loci (pmG1.1, pmG2.1, pmG2.2, pmG3.1, pmG4.1, pmG4.2, pmG5.1, pmG5.2, pmG5.3, pmG5.4, pmG6.1 and pmG6.2) associated with PM resistance were detected on all chromosomes except for Chr.7 using GWAS. Among these loci, pmG2.1, pmG4.1 and pmG6.1 were the novel ones that have not been reported in previous studies. The loci of pmG2.1, pmG5.2, pmG5.3 showed stronger signal in three seasons, for which three candidate genes Csa2G022790, Csa5G484620 and Csa5G604330 were respectively predicted using pairwise LD correlation and qRT-PCR analysis. Further, Csa4G022270 in pmG4.1, Csa6G022350 and Csa6G022370 in pmG6.1 might be the candidate genes based on the annotation of homologous genes in Arabidopsis.
Conclusion
Twelve Loci related to powdery mildew resistance in cucumber were detected by genome-wide association analysis, three of which namely pmG2.1, pmG4.1 and pmG6.1 are novel. Three candidate genes within the genomic region of locus pmG2.1, pmG5.2 and pmG5.3 were identified, respectively. These results provide targets for gene cloning and genetic breeding of PM resistance in cucumber.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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