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Research article
Identification of Potential Biomarkers for Abdominal Pain in IBS Patients by Bioinformatics Approach
Yunling dou
Sun Yat-sen University First Affiliated Hospital
Corresponding Author
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Background: Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disease characterized by chronic abdominal discomfort and pain. The mechanisms of abdominal pain, as a relevant symptom, in IBS are still unclear. We aimed to explore the key genes and neurobiological changes specially involved in abdominal pain in IBS.
Methods: Gene expression data (GSE36701) was downloaded from Gene Expression Omnibus (GEO) database. Fifty-three rectal mucosa samples from 27 Irritable Bowel Syndrome with diarrhea (IBS-D) patients and 40 samples from 21 healthy volunteers (HV) as controls were included. Differentially expressed genes (DEGs) between two groups were identified using the GEO2R online tool. Functional enrichment analysis of DEGs was performed on the DAVID database. Then a protein-protein interaction (PPI) network was constructed and visualized using STRING database and Cytoscape.
Results: The microarray analysis demonstrated a subset of genes (CCKBR, CCL13
ACPP, BDKRB2, GRPR, SLC1A2, NPFF, P2RX4, TRPA1, CCKBR, TLX2, MRGPRX3, PAX2, CXCR1) specially involved in pain transmission. Among these genes, we identified GRPR, NPFF and TRPA1 genes as potential biomarkers for irritating abdominal pain of IBS patients.
Conclusions: Overexpression of certain pain-related genes (GRPR, NPFF and TRPA1) may contribute to chronic visceral hypersensitivity, therefore be partly responsible for recurrent abdominal pain or discomfort in IBS patients. Several synapses modification and biological process of psychological distress may be risk factors of IBS.
Keywords
Bioinformatics analysis, Biomarker, Visceral hypersensitivity, Abdominal pain, Irritable bowel syndrome
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On 21 Dec, 2020
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On 21 Dec, 2020
Editor invited
On 21 Dec, 2020
No comments provided
Review #2 received
Received 14 Dec, 2020
Editorial decision: Minor revision
On 14 Dec, 2020
Review #1 received
Received 08 Dec, 2020
Reviewer #2 agreed
On 21 Nov, 2020
Reviewer #1 agreed
On 17 Nov, 2020
Reviewers invited
Invitations sent on 16 Nov, 2020
Editor assigned
On 08 Nov, 2020
Submission checks complete
On 08 Nov, 2020
Editor invited
On 08 Nov, 2020
No comments provided
Review #2 received
Received 07 Oct, 2020
Editorial decision: Major revision
On 07 Oct, 2020
Review #1 received
Received 30 Sep, 2020
Reviewer #2 agreed
On 16 Sep, 2020
Reviewer #1 agreed
On 07 Sep, 2020
Reviewers invited
Invitations sent on 06 Jul, 2020
Editor assigned
On 02 Jun, 2020
First submitted
On 01 Jun, 2020
Submission checks complete
On 01 Jun, 2020
Editor invited
On 01 Jun, 2020
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Subject Areas
Gastroenterology & Hepatology
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This preprint is under consideration at BMC Gastroenterology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Identification of Potential Biomarkers for Abdominal Pain in IBS Patients by Bioinformatics Approach
Yunling dou
Sun Yat-sen University First Affiliated Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 3
Posted 06 Jan, 2021
No community comments so far
Editor assigned
On 21 Dec, 2020
Submission checks complete
On 21 Dec, 2020
Editor invited
On 21 Dec, 2020
No comments provided
Review #2 received
Received 14 Dec, 2020
Editorial decision: Minor revision
On 14 Dec, 2020
Review #1 received
Received 08 Dec, 2020
Reviewer #2 agreed
On 21 Nov, 2020
Reviewer #1 agreed
On 17 Nov, 2020
Reviewers invited
Invitations sent on 16 Nov, 2020
Editor assigned
On 08 Nov, 2020
Submission checks complete
On 08 Nov, 2020
Editor invited
On 08 Nov, 2020
No comments provided
Review #2 received
Received 07 Oct, 2020
Editorial decision: Major revision
On 07 Oct, 2020
Review #1 received
Received 30 Sep, 2020
Reviewer #2 agreed
On 16 Sep, 2020
Reviewer #1 agreed
On 07 Sep, 2020
Reviewers invited
Invitations sent on 06 Jul, 2020
Editor assigned
On 02 Jun, 2020
First submitted
On 01 Jun, 2020
Submission checks complete
On 01 Jun, 2020
Editor invited
On 01 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Gastroenterology & Hepatology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disease characterized by chronic abdominal discomfort and pain. The mechanisms of abdominal pain, as a relevant symptom, in IBS are still unclear. We aimed to explore the key genes and neurobiological changes specially involved in abdominal pain in IBS.
Methods: Gene expression data (GSE36701) was downloaded from Gene Expression Omnibus (GEO) database. Fifty-three rectal mucosa samples from 27 Irritable Bowel Syndrome with diarrhea (IBS-D) patients and 40 samples from 21 healthy volunteers (HV) as controls were included. Differentially expressed genes (DEGs) between two groups were identified using the GEO2R online tool. Functional enrichment analysis of DEGs was performed on the DAVID database. Then a protein-protein interaction (PPI) network was constructed and visualized using STRING database and Cytoscape.
Results: The microarray analysis demonstrated a subset of genes (CCKBR, CCL13
ACPP, BDKRB2, GRPR, SLC1A2, NPFF, P2RX4, TRPA1, CCKBR, TLX2, MRGPRX3, PAX2, CXCR1) specially involved in pain transmission. Among these genes, we identified GRPR, NPFF and TRPA1 genes as potential biomarkers for irritating abdominal pain of IBS patients.
Conclusions: Overexpression of certain pain-related genes (GRPR, NPFF and TRPA1) may contribute to chronic visceral hypersensitivity, therefore be partly responsible for recurrent abdominal pain or discomfort in IBS patients. Several synapses modification and biological process of psychological distress may be risk factors of IBS.
Figure 1
Figure 2
Figure 3