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Research article
Yi Shen
Department of Orthopedics, School of Medicine, South Campus, Renji Hospital, Shanghai JiaoTong University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8635-6232
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
A successful osseointegration of total hip arthroplasty (THA) relies on the interplay of implant surface and bone marrow microenvironment. This study was undertaken to investigate the impact of perioperative biochemical molecules (Ca 2+ , Mg 2+ , Zn 2+ , VD, PTH) on the bone marrow osteogenetic factors (BMP2, BMP7, Stro-1 + cells) in the metaphyseal region of the femoral head, and further on the bone mineral density (BMD) of Gruen R3.
Methods
Bone marrow aspirates were obtained from the discarded metaphysis region of the femoral head in 51 patients with THA. Flow cytometry was used to measure the Stro-1 + expressing cells. ELISA was used to measure the concentrations of bone morphologic proteins (BMP2 and BMP7). The perioperative concentrations of the biochemical molecules above were measured by radioimmunoassay. The BMD of Gruen zone R3 was examined at 6 months after THA, using dual-energy X-ray absorptiometry (DEXA).
Results
Our data demonstrated that the concentration of Ca 2+ was positively correlated with BMP7 expression, and with the postoperative BMD of Gruen zone R3. However, the concentration of Mg 2+ had little impact on the R3 BMD, although it was negatively correlated with the expression of BMP7. The data also suggested that the other biochemical molecules, such as Zn 2+ , VD, and PTH, were not significantly correlated with any bone marrow osteogenetic factors (BMP2, BMP7, Stro-1 + cells). The postoperative R3 BMD of patients of different gender and age had no significant difference.
Conclusions
These results indicate the local concentration of Ca 2+ may be an indicator for the prognosis of THA patients.
Keywords
Uncemented THA, Ca2+, BMP7, Stro-1+ cells, osseointegration
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CURRENT STATUS: Published
View the published article at Journal of Orthopaedic Surgery and Research.
No community comments so far
Editorial decision: Accept
On 09 Nov, 2020
Reviewer #1 agreed
On 06 Nov, 2020
Review #1 received
Received 06 Nov, 2020
Editor assigned
On 04 Nov, 2020
Reviewers invited
Invitations sent on 04 Nov, 2020
Submission checks complete
On 04 Nov, 2020
Editor invited
On 04 Nov, 2020
No comments provided
Review #2 received
Received 20 Oct, 2020
Editorial decision: Major revision
On 20 Oct, 2020
Review #1 received
Received 09 Oct, 2020
Reviewer #2 agreed
On 06 Oct, 2020
Editor assigned
On 05 Oct, 2020
Reviewers invited
Invitations sent on 05 Oct, 2020
Reviewer #1 agreed
On 05 Oct, 2020
Submission checks complete
On 04 Oct, 2020
Editor invited
On 04 Oct, 2020
Version 1
Posted 08 Jun, 2020
No comments provided
Editorial decision: Major revision
On 03 Aug, 2020
Review #2 received
Received 02 Aug, 2020
Reviewer #4 agreed
On 31 Jul, 2020
Reviewer #3 agreed
On 08 Jul, 2020
Review #1 received
Received 28 Jun, 2020
Reviewer #2 agreed
On 16 Jun, 2020
Reviewers invited
Invitations sent on 15 Jun, 2020
Reviewer #1 agreed
On 15 Jun, 2020
Editor assigned
On 15 Jun, 2020
Editor invited
On 14 Jun, 2020
First submitted
On 04 Jun, 2020
Submission checks complete
On 04 Jun, 2020
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Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Orthopedic Surgery
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A new preprint design is coming!
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See the published version of this preprint at Journal of Orthopaedic Surgery and Research.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Yi Shen
Department of Orthopedics, School of Medicine, South Campus, Renji Hospital, Shanghai JiaoTong University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8635-6232
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of Orthopaedic Surgery and Research.
No community comments so far
Editorial decision: Accept
On 09 Nov, 2020
Reviewer #1 agreed
On 06 Nov, 2020
Review #1 received
Received 06 Nov, 2020
Editor assigned
On 04 Nov, 2020
Reviewers invited
Invitations sent on 04 Nov, 2020
Submission checks complete
On 04 Nov, 2020
Editor invited
On 04 Nov, 2020
No comments provided
Review #2 received
Received 20 Oct, 2020
Editorial decision: Major revision
On 20 Oct, 2020
Review #1 received
Received 09 Oct, 2020
Reviewer #2 agreed
On 06 Oct, 2020
Editor assigned
On 05 Oct, 2020
Reviewers invited
Invitations sent on 05 Oct, 2020
Reviewer #1 agreed
On 05 Oct, 2020
Submission checks complete
On 04 Oct, 2020
Editor invited
On 04 Oct, 2020
Version 1
Posted 08 Jun, 2020
No comments provided
Editorial decision: Major revision
On 03 Aug, 2020
Review #2 received
Received 02 Aug, 2020
Reviewer #4 agreed
On 31 Jul, 2020
Reviewer #3 agreed
On 08 Jul, 2020
Review #1 received
Received 28 Jun, 2020
Reviewer #2 agreed
On 16 Jun, 2020
Reviewers invited
Invitations sent on 15 Jun, 2020
Reviewer #1 agreed
On 15 Jun, 2020
Editor assigned
On 15 Jun, 2020
Editor invited
On 14 Jun, 2020
First submitted
On 04 Jun, 2020
Submission checks complete
On 04 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Orthopedic Surgery
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of Orthopaedic Surgery and Research.
Background
A successful osseointegration of total hip arthroplasty (THA) relies on the interplay of implant surface and bone marrow microenvironment. This study was undertaken to investigate the impact of perioperative biochemical molecules (Ca 2+ , Mg 2+ , Zn 2+ , VD, PTH) on the bone marrow osteogenetic factors (BMP2, BMP7, Stro-1 + cells) in the metaphyseal region of the femoral head, and further on the bone mineral density (BMD) of Gruen R3.
Methods
Bone marrow aspirates were obtained from the discarded metaphysis region of the femoral head in 51 patients with THA. Flow cytometry was used to measure the Stro-1 + expressing cells. ELISA was used to measure the concentrations of bone morphologic proteins (BMP2 and BMP7). The perioperative concentrations of the biochemical molecules above were measured by radioimmunoassay. The BMD of Gruen zone R3 was examined at 6 months after THA, using dual-energy X-ray absorptiometry (DEXA).
Results
Our data demonstrated that the concentration of Ca 2+ was positively correlated with BMP7 expression, and with the postoperative BMD of Gruen zone R3. However, the concentration of Mg 2+ had little impact on the R3 BMD, although it was negatively correlated with the expression of BMP7. The data also suggested that the other biochemical molecules, such as Zn 2+ , VD, and PTH, were not significantly correlated with any bone marrow osteogenetic factors (BMP2, BMP7, Stro-1 + cells). The postoperative R3 BMD of patients of different gender and age had no significant difference.
Conclusions
These results indicate the local concentration of Ca 2+ may be an indicator for the prognosis of THA patients.
Figure 1
Figure 2
Figure 3
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