Single-sided magnetic resonance-based sensor for point-of-care evaluation of muscle

Magnetic resonance (MR) imaging is a powerful clinical tool for the detection of soft tissue morphology and pathology, which often provides actionable diagnostic information to clinicians. Its clinical use is largely limited due to size, cost, time, and space constraints. Here, we discuss the design and performance of a low-field single-sided MR sensor intended for point-of-care (POC) evaluation of skeletal muscle in vivo. The 11kg sensor has a penetration depth of > 8 mm, which allows for an accurate analysis of muscle tissue and can avoid signal from more proximal layers, including subcutaneous adipose tissue. Low operational power and minimal shielding requirements are achieved through the design of a permanent magnet array and surface transceiver coil. We present the in vitro and human in vivo performance of the device for muscle tissue evaluation. The sensor can acquire high signal-to-noise (SNR > 150) measurements in minutes, making it practical as a POC tool for many quantitative diagnostic measurements, including T2 relaxometry.


Background
POC medical diagnostics are increasingly utilized in both inpatient and outpatient settings 1,2 .The ability to rapidly detect aneurysms, uid pockets, and other clinical ndings that can be managed using an interventional procedure can decrease the time to diagnosis and treatment, leading to improved patient outcomes 3,4 .The bedside operation of these POC instruments enables measurement of diagnostic information without the need to transport the patient to a centralized-care facility -reducing cost, time to treat, and in some cases, length of stay 5 .
Magnetic resonance imaging (MRI) is the primary clinical tool for detecting soft tissue pathology due to high soft tissue contrast.It is non-invasive, does not involve patient exposure to ionizing radiation, and allows for quanti cation of tissue morphology.Traditionally, MRI is not practical as a POC tool since the high magnetic elds (typically 1.5-3 Tesla) needed for operation present a projectile hazard for ferrous objects if operated outside of an access-controlled scanner suite.Additionally, the need for magnetic and radio frequency (RF) shielding, as well as power requirements that can exceed 25 kW, increase the footprint precluding use at the POC.It is also not compatible with patients that have certain types of metal implants; the high cost of scanner purchase and site infrastructure limitations prevent many facilities from having multiple scanners, limiting capacity despite high demand for instrument use.
Recent technical innovations in MRI physics and instrumentation have led to scanners operating at far lower magnetic elds than previously thought possible and have enabled 64 mT MRI scanners to be deployed at the patient bedside for POC use.These low-cost low-eld MRI scanners can operate without the shielding and safety requirements of traditional high-eld scanners, but their use to date has focused on neuroimaging in critical-care settings [6][7][8][9] .
Single-sided magnetic resonance (SSMR) sensors may provide a portable POC diagnostic option that leverages the power of MR-based contrast with purpose-built low-cost hand-held instruments 10 .These devices use magnetic resonance techniques to acquire spectroscopic (i.e., non-imaging) data over a limited tissue depth but have the ability to distinguish between tissue types, intra-and extra-cellular compartments and provide information about tissue architecture 11 .Analysis spatially resolved T2relaxation data from MRI has shown that the skeletal muscle compartment and the subcutaneous compartment can be represented by biexponential decays 10 .These two relaxations represent intercellular and intracellular uid.While both muscle and subcutaneous tissues exhibit biexponential T2 decay, the time constants differ between the tissues and can be distinguished from one another.
Techniques including T2 relaxometry and T2-weighted diffusion can be performed on single-sided sensors to provide clinically-actionable information [12][13][14][15] .Portable MR sensors are often constructed from permanent magnets to reduce the power requirements.Further, single-sided sensor designs do not require patient movement between rooms or beds to t inside a magnet bore 14,[16][17][18][19][20][21][22][23][24] .Previous clinical studies with SSMR sensors were limited by the penetration depth (< 6 mm) and signal sensitivity 10 .This prevented human in vivo demonstration of the diagnostic utility of SSMR devices that was demonstrated in prior ex vivo and murine studies.
We demonstrate here the design and utility of a low-eld single-sided MR sensor intended for POC evaluation of skeletal muscle in vivo 25,26 .The penetration depth is larger than other single-sided systems permitting accurate analysis of muscle tissue and avoiding signal contribution from other subcutaneous layers, including adipose tissue.Low operational power and minimal shielding requirements are achieved by constructing a permanent magnet array and surface RF coil.The sensor can acquire high SNR measurements in minutes, making it practical as a POC tool.We characterize the in vitro performance of the instrument using several multi-layer tissue phantoms.Finally, we demonstrate the sensitivity of the device to muscle sensing in vivo on a cohort of healthy human subjects.

Results
The ability to unambiguously measure magnetic relaxation properties in muscle tissue without interference from more proximal features would aid clinical evaluation of uid volume status and other applications.There are experimental challenges to realizing such a system.The homogeneous magnetic eld region of a single-sided MR system de nes the sensitive region (sweet spot) of the sensor.This must be far enough above the surface of the sensor to predominantly lie within muscle tissue for practical invivo use.The thickness of subcutaneous adipose tissue layers varies with body location, so the operation of a compact single-sided NMR sensor may be limited to those anatomical locations where the sensor's "sweet spot" depth can reach the muscle.There are select locations where, even on subjects with a higher body mass index (BMI), the subcutaneous layer rarely exceeds 6 mm 10,27,28 .We designed our sensor to operate in these types of anatomical locations, speci cally the gastrocnemius (calf) muscle.
We performed a nite-element analysis using COMSOL Multiphysics software (Burlington, MA) to design the attainable magnetic elds of the permanent magnet sensor.The net magnetic eld pro les of several magnet array con gurations were simulated to achieve a design that met our requirement for a POC sensor.Speci c endpoints, in order of priority, included homogeneous region depth from the magnet surface (> 8 mm), homogeneous region strength (> 0.2 T), homogeneous region volume (> 200 mm 3 ), weight (< 12 kg), and nal magnet array size.Computational approaches guided the design of individual magnet orientations and con gurations of the individual magnets as well as the presence, size, and positioning of iron yokes to shim the magnetic ux.We utilized the unilateral linear Halbach array and cylindrical Halbach array con gurations as the basis for our design.Individual feature parameters were swept to achieve a large volume homogeneous region while minimizing stray elds outside of that region.The magnet array of the sensor device (Fig. 1) is designed to comfortably seat the calf muscle, allowing for muscle measurements with less variability due to leg placement against the sensor.
The magnet array is constructed from half-inch cube N52 neodymium magnets deployed in machined aluminum frames.The magnets used in the nal array were pre-screened and selected for homogeneity.
Iron yokes with thickness of 1-mm were placed on the inner raised Halbach elements to increase the volume of the homogeneous region.The mapped homogeneous region following fabrication has a eld strength of 0.2 T and sits 8mm above the surface of the array with a natural descending gradient in the Z direction of 1 T/m.A single surface transceiver coil is used for the pulsed magnetic eld 29,30 .A surface coil allows the calf of a subject to be placed directly in contact with the sensor and is agnostic to body size and placement.The assembled sensor with Delrin casing weighs 11 kg and is 22 cm length x 17.4cm in width, and 11 cm in height.

Clinical design aspects
The ability to acquire data with high SNR enables short acquisition times which is an important clinical consideration for SSMR sensors.The sensors must maintain high signal delity in different environments such as hospitals, outpatient centers, homes, etc. in the presence of a variety of other equipment generating uncontrolled EMI interference.
Inductive and capacitive coupling between the RF coil and the subject can increase the RF noise, making reliable grounding strategies critical.The Delrin casing around the completed magnet array was designed with six copper grounding rods with 5-mm-diameter that contact the bottom surface of the aluminum frame of the magnets on one end and contact with the external aluminum plate on the other end, where the sensor is placed.The copper rods in our design sit on a grounded aluminum plate to ensure the magnets are grounded.The 'L' impedance matching network is set in a 3D printed polylactic acid (PLA) casing with a thin aluminum housing.This allows a human subject to be grounded through contact of the leg with a metal casing around the network.The grounding con guration signi cantly decreases baseline noise for human subjects.Noise levels are approximately 0.22 µV without a human subject and 0.40 µV with a human subject in contact with the sensor (Fig. 2A and 2B).
Transceiver coil heating is a critical concern for the clinical implementation of SSMR 31,32 .High-power RF pulse trains generate Joule heating which causes the coil to heat to a temperature that must be mitigated for human use.The acquired signal is insu cient for meaningful measurements within reasonable scan times if the pulse power is decreased.This is addressed by conducting the heat to the magnet through a thermally conductive path.Aluminum nitride (AlN) is a high thermal conductivity dielectric.AlN sheets (0.25-1.2 mm-thick) were machined to t around the coil, with an additional 0.25 mm sheet placed over the top of the coil.The AlN sheets direct the heat generated by the pulse trains away from the human subject and into the body of the aluminum magnet frame.The AlN sheets surrounding the coil also prevent human subjects from coming in direct contact with the coil.This approach prevents sample heating of more than 1 ℃ over 10 min of continuous signal acquisition, as displayed in Fig. 2C.
In vitro, characterization of the sensor demonstrates sensitivity in the sensitive region A CPMG pulse sequence was used to acquire T2 relaxometry data.Characterization of the sensor's depth sensitivity was achieved with copper sulfate phantoms.Slice selection characterization was performed by adjusting the pulsed (B1) frequency from 8.32-8.42MHz in 0.1 MHz increments.A 1 M copper sulfate sample was measured at each frequency in a machined PEEK case with a 1mm sample height.The sample was raised in 1mm increments perpendicularly above the surface of the array and signal was acquired at each sample position.The relation between B1 frequency and depth sensitivity is shown in Fig. 3.
Custom tissue phantoms were made to more accurately evaluate the ability of the sensor to capture signals from muscle tissue while avoiding signals from subcutaneous adipose tissue.These phantoms consist of two compartments, oil, and water, emulsi ed with different percentages of the components 33 .The phantoms mimic the intra-and inter-cellular compartments of both muscle and adipose tissue.They have similar T2 relaxation times and differing relative amplitudes when analyzed with a bi-exponential t due to the different contributions of the oil and water.Individual phantoms were created for adipose tissue only, muscle tissue only, and layered adipose (6mm thick) with muscle tissue over.Signals from the adipose and muscle tissue phantoms were used as the standard for the subsequent layered phantom tests.The adipose layer of the phantoms can be poured with variable thicknesses to mimic differing amounts of subcutaneous adipose tissue between the surface of the magnet array and the skeletal muscle from which we obtain signal measurements.
Data was collected from the phantoms at 8.48, 8.43, 8.38, and 8.29 MHz, corresponding to depths of approximately 2-, 5-, 8-, and 10-mm from the surface of the sensor.The relative signal magnitude of the second component of a biexponential t of the T2 decays verify, shown in Fig. 4, veri es the slice selectivity and ability to capture the signal from deeper skeletal muscle phantom while avoiding the 6mm thick subcutaneous adipose tissue layer, shown in Fig. 4. We select the sampling region of the signal based on the permanent magnetic eld pro le by altering the frequency of the RF pulses.Decreasing the frequency allows for signal sampling further from the surface of the magnet array and deeper into the tissue.The signal from the layered phantom acquired at and below 8.38MHz is statistically the same as the muscle tissue phantom.This demonstrates we are only capturing the signal from the muscle portion of the layered phantom.The signal re ects amplitudes between the muscle and adipose phantoms at 8.43 MHz.The signal at this frequency contains contributions from both phantom types near the layer junction.The signal acquired at 8.48 MHz, however, statistically re ects the adipose phantom; verifying that we are fully below the phantom layer junction.We can achieve an accurate signal from muscle phantom above a 6-mm-thick layer of adipose phantom at 8.38 MHz using a biexponential t of the decay.
In-vivo use demonstrates expected muscle values compared to excised murine tissue Subjects placed their leg on the sensor and were scanned for 10 minutes to produce a high SNR (> 150) signal.Separately, adipose (axillary and inguinal site) and muscle (gastrocnemius and soleus) tissue were excised from rodents by a veterinary technician.This ex-vivo murine tissue was placed directly on an SSMR sensor to obtain 'standard' SSMR relaxometry measurements for muscle tissue and adipose tissue individually.In-vivo human measurements were compared to the ex-vivo murine tissue to determine the ability of the device to capture in-vivo signals from muscle tissue.The data was analyzed with bi-exponential t to determine if the human data captured resembles muscle tissue.The biexponential t captures the intra-and extra-cellular compartments of tissue.There is a statistically signi cant difference between the in-vivo human signal and the ex-vivo adipose signal for both the T2 times and relative amplitudes.Three of the four values comparing in-vivo human signal and ex-vivo muscle tissue have statistical similarity, demonstrating that we successfully capture signals from in-vivo muscle tissue of human subjects.

Discussion
The ideal diagnostic tool provides rapid and actionable information to clinicians without disruption to the patient.MRI is a common noninvasive diagnostic tool for soft tissue, but except for recent low-eld instantiations, the size, cost, and safety considerations of these machines signi cantly limit POC use.Single-sided MR tools can provide localized NMR measurements close to the surface of the sensor.
Depending on the RF pulse sequence utilized, these sensors can be sensitive to T1 or T2 relaxation and diffusion.These methods provide different information about tissue architecture and pathology.
Single-sided MR sensors are not currently used in clinical practice.Several limitations exist, including the measurement depth, acquisition time, and validation of signal sensitivity for clinical decision making.To date, no single-sided sensor has been designed with the penetration depth and low gradient necessary for clinical implementation, especially for single-voxel measurements.We maximize the signal acquisition region by minimizing the gradient.Other single-sided sensors have signi cantly higher magnetic eld gradients, which increases slice selectivity but reduces the signal intensity and sensitive volume.
We demonstrate the design and construction of an MR sensor for in vivo detection and evaluation of skeletal muscle.Our single-sided MR sensor was designed with permanent magnets to have an easy to fabricate static magnetic eld.The magnet array is a unilateral Linear Halbach design with raised edges which offers a remote, low eld, low gradient, homogenous region.The Halbach array is well suited for POC settings because the magnetic eld is limited to only one side of the array, eliminating the effects of stray elds and the need for specialized shielding and shimming.The sensitive region of this sweet B 0 spot magnet is 8 mm from the surface of the magnet.The magnetic eld produced by this design is aligned parallel to the surface of the sensor.That eld direction permits a standard surface transceiver coil on the surface of the magnet.This region contains muscle when placed against a human's leg.The magnet array produces a magnetic eld gradient that decreases in the direction of depth into the tissue.The frequency of the RF pulse required to excite a proton depends on eld strength.A Car-Purcell-Meiboom-Gill (CPMG) Pulse sequence is used for signal acquisition to maximize the SNR due to its insensitivity to eld homogeneities.The sensor was designed to acquire high SNR signals with highpower RF pulses without inducing discomfort caused by coil heating through the inclusion of aluminum nitride sheets encasing the RF coil.Grounding lines for the portable system are constructed within the array casing to reduce external wiring, provide reproducible grounding solutions, and increase exibility and portability.
The CPMG sequence captures T2 relaxation times and amplitudes, which allows for tissue analysis.T2 times are tissue compartment dependent as this relaxation property is the function of the microstructure and composition of the tissue.Proton spins are the origin of the T2 signal, almost all of which are from water.Different characteristics of water within the body result in different T2 times; the protons in pure water, water bound to macromolecules, interstitial, and intracellular water all diphase at different rates which allows them to be separated and quanti ed.This measurement was investigated previously for invivo uid estimation, and tissue identi cation, and can be used for several other tissue architecture questions.
We created tissue phantoms to validate the sensitivity of our sensor.Layers of adipose and muscle in a multi-phase phantom were fabricated to demonstrate the acquisition of the muscle "signature" without collecting signals from the more proximal adipose layer.We then demonstrated our device's ability to collect a muscle signature from a cohort of healthy volunteers.Subjects placed their calf on the sensor from a seated position.A 10-minute acquisition time was su cient to acquire a high SNR signal (> 150) in muscle tissue.The T2 times and relative amplitudes obtained from the t re ect expected values for muscle tissue in direct comparison to excised murine muscle and adipose tissue.This is the rst published instance we have identi ed of single-sided muscle detection in humans.Ultimately, our intention is to minimize scan time while also reliably producing an SNR of 200.This can be acquired over approximately 6 min based on our initial data and we anticipate further SNR increases which would continue decreasing the necessary acquisition time.This paper presents the design and construction of an SSMR sensor for in vivo muscle detection.The permanent magnet array creates a 0.2 T homogeneous region lifted 8mm above the surface of the magnet.A small surface coil transmits high-power pulses in long echo trains enabling T2 relaxometry measurements.Tissue heating is avoided by encasing the coil in aluminum nitride.Our sensor was characterized and validated with complex layered tissue phantoms representing subcutaneous adipose tissue and muscle.Finally, we tested the sensor on a small cohort of healthy human subjects to determine if we could successfully detect muscle tissue.Future studies include additional evaluation of diagnostic utility and design and application of complex RF pulses.

FEA design
The desired single-sided magnet array has a sweet spot (homogeneous region) eld strength of at least 0.2 T lifted at least 6mm above the surface of the magnet.COMSOL Multiphysics was used to perform nite element analysis.The permanent magnetic eld was generated using the magnetic elds, no currents module which uses to generate the eld pro le.Remanent ux density and relative permeability were determined by the values for N52 neodymium magnets (1.48 T and unity).An extra-ne physics de ned mesh was applied to the geometry to solve Gauss' Law.The magnet array geometry was placed inside a large rectangular prism with no magnetization and air to provide the simulation environment.Sweet spot volume ( = 0.5%), strength (T), and depth (mm) were used as outcome measures.Magnet size, orientation, location, spacing, etc were input parameters.These were swept to determine optimal magnet array design.

Fabrication
The nal magnet array design consists of 448 individual N52 (Nd1Fe14B) magnets.Each magnet is 12.7 mm cubed.One thousand serialized magnets (Viona Magnetics, Hicksville, NY) were individually ux tested with a hall probe and gauss meter (Lake Shore Cryotronics, Woburn, MA).Of those, the most homogeneous were selected for inclusion in the constructed array.
Each magnet was secured in a machined aluminum frame (Xometry, North Bethesda, MD).Two frame styles were designed, the center style holds 32 magnets arranged in 4 rows of 8, and the end style holds 48 magnets in 6 rows of 8.In total, 4 end and 8 center frames were machined.Cube magnets were placed in the aluminum frame and covered with a temporary aluminum cover slip.The cover slip prevented individual magnets from ejecting, due to repulsion, from the frame during subsequent magnet placement.
All magnets in a frame are oriented in the same direction according to the gure.There is a 1mm gap between each of the magnets in the frame.
A temporary structure was made using three-foot aluminum rods secured in a 6 in 12 in aluminum block to align the magnet frames together.The bolt holes on each array frame were used to slide the frames along the rods without allowing for sideslipping in any direction due to the strong magnetic forces.The temporary cover slips over the magnets were removed once all 12 frames were aligned on the rods.The aluminum rods were removed one at a time and replaced with brass bolts.Machined Delrin (Xometry, North Bethesda, MD) is used to encase the aluminum magnet array and block stray magnetic elds.
A transceiver coil was constructed from AWG32 magnet wire (MWS Wire, Oxnard, CA) wound around a cylindrical Te on former (McMaster Carr, Elmhurst, IL).The coil has 8 turns and is 16mm in diameter.The coil is connected to an 'L' impedance matching network with capacitances selected for our desired frequency range (8.2-8.5 MHz).

Phantom construction
Multi-phase tissue phantoms were fabricated according to the general protocols described in Bush et al. 33 This emulsion phantom protocol allows for multi-component analysis and more accurate physiological features.The protocol was modi ed to fabricate phantoms for different tissue types by adjusting the percentage of oil and aqueous components.A 40 %oil fraction was used for muscle tissue and a 70 %oil fraction was used for adipose tissue.
The aqueous phase components consist of deionized (DI) water, sodium benzoate, Tween-20, and agar (Sigma-Aldrich, St. Louis, MO).To prepare 100 mL of the aqueous phase, 100 mL of DI water was added to a 400 mL beaker.The beaker was placed on a hotplate set at 90 ℃with a stir rate of 100 rpm.0.1 g of sodium benzoate was measured and added to the water, followed by 0.2 mL of the water-soluble surfactant.Next, 3.0 g of agar was slowly added to the water beaker.Once added, the hotplate temperature was increased to 350 ℃and the stir bar speed increased to 1100 rpm for 5-10 min to melt the agar.The solution was removed from the hotplate to check for clear color, no dispersed air bubbles, and no clumps or streams of agar.The aqueous solution was tested to ensure the agar melted by placing about 5 mL of solution in a separate glass vial.If the solution set and was clear, then the solution was then placed back on the hotplate (50 ℃and 100 rpm) while the oil solution was prepared.If the separated solution did not set, the hotplate temperate was increased and the agar was given more time to melt before proceeding.
The oil solution consists of peanut oil and Span 80 (Sigma-Aldrich, St. Louis, MO).To prepare 100 mL of the oil solution, 100 mL of peanut oil was measured and placed in a clean beaker with a clean stir bar.
The beaker was placed on a hotplate set at 90 ℃with a stir rate of 100 rpm for 1 min.1.0 mL of the oilsoluble surfactant was added dropwise to the beaker with peanut oil.The hotplate settings were increased to 150 ℃and 1100 rpm for 5 minutes to fully mix the oil solution.
To create the phantom emulsion, a clean stir bar was placed in a 250 mL Erlenmeyer ask.A volumetric pipette was used to add the appropriate amount of the aqueous solution to the ask (amount of solution added depends on oil fraction of phantom being created).For example, to create 100 ml of a 40% phantom, 60 ml of aqueous solution was added to the ask.The ask was placed on a hotplate set at 90 ℃and 1100 rpm.After 2 min of stirring, 40 ml of the oil solution was measured with a volumetric pipette and slowly added dropwise (around 1 drop per second for emulsions at a fat fraction of 35% or greater) to the aqueous solution in the ask.When streaks of oil were observed in the emulsion, no further oil was added until stirring had fully emulsi ed the separated oil.Once all the oil solution was added, the hotplate settings were adjusted to 300 ℃ and 1100 rpm and the emulsion was stirred for 5 min.The emulsion was white, with a creamy and smooth texture with no visible separated oil.The emulsion was then poured into glass vials to cool and set.
Layered Phantoms: Layered phantoms were constructed using the protocol outlined above.To mimic a human leg on a single-sided sensor, the phantom required an adipose layer closer to the surface of the magnet and a muscle layer above.Adipose tissue phantoms were constructed, poured into a vial with depths ranging between 1 and 8 mm, at 1 mm increments, and set overnight in a refrigerator.The following day, muscle phantom was created and poured in a layer immediately on top of the adipose phantom, and re-set in a refrigerator.Melting, phase separation, or mixing of the two phantoms layers was not observed.Layered phantoms were created for varying levels of subcutaneous adipose layer thickness from 1 to 8 mm.

Signal Acquisitions:
A Kea2 spectrometer (Magritek, Wellington, New Zealand) is used to acquire signal.Prospa software (Magritek, Wellington, New Zealand) provides the setup and analysis interface.The internal spectrometer RF ampli er is connected via coaxial cable to the matching network.
Diffusion: T2-weighted diffusion measurements were performed with serial CPMG sequences with varying echo times ranging from 65 µs -1020 µs.Changes in echo time of the CPMG sequence alter the signal attenuation due to differences in sample diffusivity, allowing for assessment of sample diffusivity without the use of gradients.

Mapping
The magnetic eld pro le of each individual magnet frame, and the nal constructed array were characterized with a hall probe (HMMY-6J04-VR, Lake Shore Cryotronics, Woburn, MA) and gaussmeter (Model 475 DSP Gaussmeter, Lake Shore Cryotronics, Woburn, MA).A 16 16 32 mm area in the center of the array containing the array sweet spot was scanned at 1 mm intervals (Figure S3).

CuSO4 Sensitivity Pro le
Pro les for signal sensitivity vs. depth from the surface of the magnet were performed to determine the optimal frequency for signal acquisition.A PEEK holder was machined with a 1 mm 16 mm 32 mm pocket.The pocket was lled with 1MCuSO4, secured to a robotic arm, and positioned directly above the top of a surface RF coil placed on the center of the magnet array.
The sensitivity pro le was performed by scanning the CuSO4 sample along a perpendicular line at distances of 6-14mm above the surface of the magnet in 1mm increments.This process was repeated using B1 frequencies from 8.32-8.42MHz in 0.01 MHz intervals.
Each scan was acquired with a Kea2 spectrometer (Magritek, Wellington, New Zealand) using a CPMG pulse sequence with 8192 echoes, 65 µs echo time, 12 µs pulse duration.
Ex vivo Muscle (gastrocnemius and soleus) and adipose (axillary and inguinal) tissue was excised from rats by a veterinary technician immediately following euthanasia.Excised tissue was wrapped in phosphate buffered saline (PBS) soaked gauze, placed on ice, and transported for immediate MR characterization.Ex-vivo murine tissue MR characterization was performed on a different SSMR than the one described in this manuscript.The sensor used, described in Colucci et al, functions at a different operating frequency.
The same pulse sequence and data tting methods were used on the two instruments 10 .
Signal from 26 different CuSO4 Phantoms at concentrations between 0.001M and 0.2M were acquired on the two SSMR sensors to compare the T2 relaxation times between the two sensors.The relation between the T2 times on each magnet array, Fig. S5, was used to perform a direct comparison between ex-vivo data captured on the existing magnet array and the in-vivo data captured on the magnet array described in this manuscript.We expect the shorter T2 times on the existing sensor due to greater in uence from the less homogeneous eld and T2*.T2 relaxation times of tissue acquired on the sensor described in Colucci et al. were offset using the equation of where is the relaxation time on the sensor reported by Colucci et al 10 .and is the relaxation time on the sensor reported in this study.This allows for a direct comparison to the signal acquired on the sensor described in this manuscript.

In vivo
Human subjects are asked to sit and place one leg on the MR sensor and the other leg next to the sensor.For each human subject, the matching network is tuned to minimize impedance at our working frequency, and baseline noise with the human subject is recorded.A CPMG pulse sequence, described previously, is used to acquire T2 decay signals.Individual scans of 16 averages take approximately one minute to complete; each individual scan is repeated 10 times, for a total data collection time of nearly 10 minutes for each subject.(MIT IRB protocol 2002000099) Fitting & Analysis T2 decay curves obtained from each scan are modeled as bi-exponential signals.The T2 times and relative amplitudes of the signal components are determined by tting the data to a bi-exponential decay curve with the highest R-squared value and subsequently analyzed.Echo integrals are computed as the sum of the points sampled for each echo during CPMG when more than one point was collected for each echo.A general multicomponent exponential decay signal is represented as: where is the estimated signal, is the number of components, is a vector of amplitudes and is a vector of corresponding relaxation times.See image above for gure legend.
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