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Research Article
Yong-Wei Fu
The First People’s Hospital of Lianyungang (Lianyungang Clinical Medical College of Nanjing Medical University)
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Although deubiquitinating enzymes (DUBs) such as CYLD, A20 and OTULIN are expressed in multiple tissues and thought to be linked with inflammatory diseases, their expression in periodontal tissues remains to be determined. This research was designed to assess the expression of CYLD, A20 and OTULIN in human gingiva, and to evaluate the regulation of these DUBs in human gingival fibroblasts (HGFs) upon different stimuli.
Methods
Immunohistochemistry assay was conducted to determine the expression of CYLD, A20 and OTULIN in human gingiva. Immunofluorescence assay was employed to observe the protein expression of CYLD, A20 and OTULIN in HGFs. RT-PCR and western blots were carried out to assess gene and protein expression changes of these DUBs in HGFs upon LPS or TNF-α.
Results
CYLD, A20 and OTULIN were found to be expressed in human gingiva and HGFs. Further, the expression of CYLD, A20 and OTULIN in HGFs exhibited distinct regulation by different stimuli.
Conclusions
Our findings suggest that CYLD, A20 and OTULIN might play a role in the progression of periodontitis.
Keywords
CYLD, A20, OTULIN, Gingiva, Fibroblasts
Figure 1
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No competing interests reported.
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Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 23 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 12 Apr, 2021
Reviews received
Received 09 Apr, 2021
Reviews received
Received 25 Mar, 2021
Reviewers agreed
On 23 Mar, 2021
Reviewers agreed
On 21 Mar, 2021
Reviewers invited
Invitations sent on 21 Mar, 2021
Editor assigned
On 21 Mar, 2021
Editor invited
On 18 Mar, 2021
Submission checks complete
On 18 Mar, 2021
First submitted
On 16 Mar, 2021
Metrics
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PDF Downloads: ...
HTML Views: ...
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This preprint is under consideration at BMC Oral Health. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Yong-Wei Fu
The First People’s Hospital of Lianyungang (Lianyungang Clinical Medical College of Nanjing Medical University)
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 23 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 12 Apr, 2021
Reviews received
Received 09 Apr, 2021
Reviews received
Received 25 Mar, 2021
Reviewers agreed
On 23 Mar, 2021
Reviewers agreed
On 21 Mar, 2021
Reviewers invited
Invitations sent on 21 Mar, 2021
Editor assigned
On 21 Mar, 2021
Editor invited
On 18 Mar, 2021
Submission checks complete
On 18 Mar, 2021
First submitted
On 16 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Although deubiquitinating enzymes (DUBs) such as CYLD, A20 and OTULIN are expressed in multiple tissues and thought to be linked with inflammatory diseases, their expression in periodontal tissues remains to be determined. This research was designed to assess the expression of CYLD, A20 and OTULIN in human gingiva, and to evaluate the regulation of these DUBs in human gingival fibroblasts (HGFs) upon different stimuli.
Methods
Immunohistochemistry assay was conducted to determine the expression of CYLD, A20 and OTULIN in human gingiva. Immunofluorescence assay was employed to observe the protein expression of CYLD, A20 and OTULIN in HGFs. RT-PCR and western blots were carried out to assess gene and protein expression changes of these DUBs in HGFs upon LPS or TNF-α.
Results
CYLD, A20 and OTULIN were found to be expressed in human gingiva and HGFs. Further, the expression of CYLD, A20 and OTULIN in HGFs exhibited distinct regulation by different stimuli.
Conclusions
Our findings suggest that CYLD, A20 and OTULIN might play a role in the progression of periodontitis.
Figure 1
Figure 2
Figure 3
Figure 4
No competing interests reported.
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