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Research
Xi Yang
Huzhou Central Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2382-0282
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Patients with colorectal cancer (CRC) are at risk of malnutrition. Gut microbes and microbial metabolites are involved in the initiation and development of CRC.
Purpose
To investigate serum protein levels in CRC patients and explore the role of gut microbes and microbial metabolites in CRCs complicated with different serum albumin levels.
Methods
Overall, 398 CRC patients and same number of healthy volunteers in Huzhou Central Hospital from January 2016 to December 2018 were recruited to compare serum protein levels. The serological indicators were detected by Abbott Automatic Biochemical Analyzer(HCHL-YQ-SH-01). A total of 30 and 56 stool samples from CRC patients were used to detect intestinal microbes and microbial metabolites, respectively. Bacterial 16S V3-V4 and fungal ITS ribosomal DNA genes were sequenced and gas chromatography/mass spectrometry (GC/MS) was performed to detect microbial metabolites.
Results
Some serum protein-related indicators in the CRC group were lower than those in the control group (p༜0.05). The total protein and albumin levels in colon cancer patients were lower than those in rectal cancer patients (p༜0.05). The higher abundance of Sutterella is correlated with lower serum albumin level in CRCs. There were statistically significant differences in the abundance of fungi including Agaricomycetes, Simplicillium, Sclerotiniaceae, and Exophiala among patients with different serum albumin levels. Multiple gut bacteria and fungi are closely related to serum albumin levels.We found some characteristic microbial metabolites in CRCs complicated with different serum protein levels.
Conclusions
The different serum albumin levels were associated with the gut microbes and microbial metabolites in CRCs. It may provide novel ideas for basic research and clinical application.
Keywords
Bacteria, fungus, Metabolites, Albumin, Colorectal Cancer
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This is a preprint. It has not completed peer review.
Research
Xi Yang
Huzhou Central Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2382-0282
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 11 Jun, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Nutrition & Dietetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Patients with colorectal cancer (CRC) are at risk of malnutrition. Gut microbes and microbial metabolites are involved in the initiation and development of CRC.
Purpose
To investigate serum protein levels in CRC patients and explore the role of gut microbes and microbial metabolites in CRCs complicated with different serum albumin levels.
Methods
Overall, 398 CRC patients and same number of healthy volunteers in Huzhou Central Hospital from January 2016 to December 2018 were recruited to compare serum protein levels. The serological indicators were detected by Abbott Automatic Biochemical Analyzer(HCHL-YQ-SH-01). A total of 30 and 56 stool samples from CRC patients were used to detect intestinal microbes and microbial metabolites, respectively. Bacterial 16S V3-V4 and fungal ITS ribosomal DNA genes were sequenced and gas chromatography/mass spectrometry (GC/MS) was performed to detect microbial metabolites.
Results
Some serum protein-related indicators in the CRC group were lower than those in the control group (p༜0.05). The total protein and albumin levels in colon cancer patients were lower than those in rectal cancer patients (p༜0.05). The higher abundance of Sutterella is correlated with lower serum albumin level in CRCs. There were statistically significant differences in the abundance of fungi including Agaricomycetes, Simplicillium, Sclerotiniaceae, and Exophiala among patients with different serum albumin levels. Multiple gut bacteria and fungi are closely related to serum albumin levels.We found some characteristic microbial metabolites in CRCs complicated with different serum protein levels.
Conclusions
The different serum albumin levels were associated with the gut microbes and microbial metabolites in CRCs. It may provide novel ideas for basic research and clinical application.
Figure 1
Figure 2
Figure 3
Figure 4
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