Autologous plasma versus fetal calf serum as a supplement for the culture of neutrophils.
Objective Currently the replacement of fetal calf serum (FCS) by a more suitable alternative is a sought aim in the field of tissue and cell culture research. Autologous plasma (AP) and especially autologous serum (AS) have been shown to be effective substitutes of FCS in culture media for some of cell types. Nevertheless, there is no comparative data on the most appropriate supplement for cell media in neutrophil studies, it is now unclear whether AP have relatively an equal, superior or inferior performance to FCS in neutrophil cell culture. In the present study, human blood neutrophils were isolated and cultured in FCS- or AP-supplemented medium. After 12, 36 and 60 hours of incubation, cell viability, oxidative burst and CD11b expression were determined by flow cytometry.Results Compared to the culture of neutrophils in FCS 10% medium, the culture of neutrophils in a medium with AP 10% could prolong their life span without affecting their function. The findings introduce AP as a better supplement for human neutrophil cell culture than FCS and propose a simple and economical procedure for neutrophil isolation and culture.
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Posted 13 Jan, 2020
On 22 Jan, 2020
On 10 Jan, 2020
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On 30 Dec, 2019
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On 29 Dec, 2019
On 18 Dec, 2019
Received 17 Dec, 2019
On 05 Dec, 2019
Invitations sent on 04 Dec, 2019
On 02 Dec, 2019
On 01 Dec, 2019
On 01 Dec, 2019
On 30 Nov, 2019
On 13 Nov, 2019
On 02 Sep, 2019
Received 20 Aug, 2019
On 05 Aug, 2019
On 05 Aug, 2019
On 05 Aug, 2019
On 05 Aug, 2019
Invitations sent on 05 Aug, 2019
On 04 Aug, 2019
Autologous plasma versus fetal calf serum as a supplement for the culture of neutrophils.
Posted 13 Jan, 2020
On 22 Jan, 2020
On 10 Jan, 2020
On 09 Jan, 2020
On 03 Jan, 2020
On 30 Dec, 2019
On 29 Dec, 2019
On 29 Dec, 2019
On 18 Dec, 2019
Received 17 Dec, 2019
On 05 Dec, 2019
Invitations sent on 04 Dec, 2019
On 02 Dec, 2019
On 01 Dec, 2019
On 01 Dec, 2019
On 30 Nov, 2019
On 13 Nov, 2019
On 02 Sep, 2019
Received 20 Aug, 2019
On 05 Aug, 2019
On 05 Aug, 2019
On 05 Aug, 2019
On 05 Aug, 2019
Invitations sent on 05 Aug, 2019
On 04 Aug, 2019
Objective Currently the replacement of fetal calf serum (FCS) by a more suitable alternative is a sought aim in the field of tissue and cell culture research. Autologous plasma (AP) and especially autologous serum (AS) have been shown to be effective substitutes of FCS in culture media for some of cell types. Nevertheless, there is no comparative data on the most appropriate supplement for cell media in neutrophil studies, it is now unclear whether AP have relatively an equal, superior or inferior performance to FCS in neutrophil cell culture. In the present study, human blood neutrophils were isolated and cultured in FCS- or AP-supplemented medium. After 12, 36 and 60 hours of incubation, cell viability, oxidative burst and CD11b expression were determined by flow cytometry.Results Compared to the culture of neutrophils in FCS 10% medium, the culture of neutrophils in a medium with AP 10% could prolong their life span without affecting their function. The findings introduce AP as a better supplement for human neutrophil cell culture than FCS and propose a simple and economical procedure for neutrophil isolation and culture.
Figure 1
Figure 2
Figure 3