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Abbasali Pourazar
Corresponding Author
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Objective Currently the replacement of fetal calf serum (FCS) by a more suitable alternative is a sought aim in the field of tissue and cell culture research. Autologous plasma (AP) and especially autologous serum (AS) have been shown to be effective substitutes of FCS in culture media for some of cell types. Nevertheless, there is no comparative data on the most appropriate supplement for cell media in neutrophil studies, it is now unclear whether AP have relatively an equal, superior or inferior performance to FCS in neutrophil cell culture. In the present study, human blood neutrophils were isolated and cultured in FCS- or AP-supplemented medium. After 12, 36 and 60 hours of incubation, cell viability, oxidative burst and CD11b expression were determined by flow cytometry.Results Compared to the culture of neutrophils in FCS 10% medium, the culture of neutrophils in a medium with AP 10% could prolong their life span without affecting their function. The findings introduce AP as a better supplement for human neutrophil cell culture than FCS and propose a simple and economical procedure for neutrophil isolation and culture.
Keywords
Fetal calf serum, Autologous plasma, Apoptosis, Granulocytes
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CURRENT STATUS: Published
View the published article at BMC Research Notes.
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Posted 13 Jan, 2020
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On 10 Jan, 2020
Editorial decision: Accept
On 09 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 03 Jan, 2020
Editor assigned
On 30 Dec, 2019
Submission checks complete
On 29 Dec, 2019
Editor invited
On 29 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 18 Dec, 2019
Review #1 received
Received 17 Dec, 2019
Reviewer #1 agreed
On 05 Dec, 2019
Reviewers invited
Invitations sent on 04 Dec, 2019
Editor assigned
On 02 Dec, 2019
Submission checks complete
On 01 Dec, 2019
Editor invited
On 01 Dec, 2019
No comments provided
Editorial decision: Not provided
On 30 Nov, 2019
Submission checks complete
On 13 Nov, 2019
No comments provided
Editorial decision: Major revision
On 02 Sep, 2019
Review #1 received
Received 20 Aug, 2019
Reviewer #1 agreed
On 05 Aug, 2019
Submission checks complete
On 05 Aug, 2019
Editor invited
On 05 Aug, 2019
Editor assigned
On 05 Aug, 2019
Reviewers invited
Invitations sent on 05 Aug, 2019
First submitted
On 04 Aug, 2019
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Subject Areas
Animal Science
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See the published version of this preprint at BMC Research Notes.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research note
Abbasali Pourazar
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Research Notes.
Version 5
Posted 13 Jan, 2020
No community comments so far
Submission checks complete
On 10 Jan, 2020
Editorial decision: Accept
On 09 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 03 Jan, 2020
Editor assigned
On 30 Dec, 2019
Submission checks complete
On 29 Dec, 2019
Editor invited
On 29 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 18 Dec, 2019
Review #1 received
Received 17 Dec, 2019
Reviewer #1 agreed
On 05 Dec, 2019
Reviewers invited
Invitations sent on 04 Dec, 2019
Editor assigned
On 02 Dec, 2019
Submission checks complete
On 01 Dec, 2019
Editor invited
On 01 Dec, 2019
No comments provided
Editorial decision: Not provided
On 30 Nov, 2019
Submission checks complete
On 13 Nov, 2019
No comments provided
Editorial decision: Major revision
On 02 Sep, 2019
Review #1 received
Received 20 Aug, 2019
Reviewer #1 agreed
On 05 Aug, 2019
Submission checks complete
On 05 Aug, 2019
Editor invited
On 05 Aug, 2019
Editor assigned
On 05 Aug, 2019
Reviewers invited
Invitations sent on 05 Aug, 2019
First submitted
On 04 Aug, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Animal Science
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Research Notes.
Objective Currently the replacement of fetal calf serum (FCS) by a more suitable alternative is a sought aim in the field of tissue and cell culture research. Autologous plasma (AP) and especially autologous serum (AS) have been shown to be effective substitutes of FCS in culture media for some of cell types. Nevertheless, there is no comparative data on the most appropriate supplement for cell media in neutrophil studies, it is now unclear whether AP have relatively an equal, superior or inferior performance to FCS in neutrophil cell culture. In the present study, human blood neutrophils were isolated and cultured in FCS- or AP-supplemented medium. After 12, 36 and 60 hours of incubation, cell viability, oxidative burst and CD11b expression were determined by flow cytometry.Results Compared to the culture of neutrophils in FCS 10% medium, the culture of neutrophils in a medium with AP 10% could prolong their life span without affecting their function. The findings introduce AP as a better supplement for human neutrophil cell culture than FCS and propose a simple and economical procedure for neutrophil isolation and culture.
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
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