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Clematis species are commonly grown in western and Japanese gardens. Heat stress can inhibit many physiological processes mediating plant growth and development. The mechanism regulating responses to heat has been well characterized in Arabidopsis thaliana and some crops, but not in horticultural plants, including Clematis species. In this study, we found that Clematis alpina ‘Stolwijk Gold’ was heat-sensitive whereas Clematis vitalba and Clematis viticella ‘Polish Spirit’ were heat-tolerant based on the physiological analyses in heat stress. Transcriptomic profiling identified a set of heat tolerance-related genes (HTGs). Consistent with the observed phenotype in heat stress, 41.43% of the differentially expressed HTGs between heat treatment and control were down-regulated in heat-sensitive cultivar Stolwijk Gold, but only 9.80% and 20.79% of the differentially expressed HTGs in heat resistant C. vitalba and Polish Spirit, respectively. Co-expression network, protein–protein interaction network and phylogenetic analysis revealed that the genes encoding heat shock transcription factors (HSFs) and heat shock proteins (HSPs) played an essential role in Clematis resistance to heat stress. Ultimately, we proposed that two clades of HSFs may have diverse functions in regulating heat resistance from C. vitalba and CvHSFA2-2 could endow different host with high temperature resistance. This study provides first insights into the diversity of the heat response mechanisms among Clematis species.
Keywords
Clematis, Heat stress, Transcriptome, HSFs, HSPs, VIGS
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- Additionalfile5SupplementaryTable1.xlsx
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View the published article at Plant Molecular Biology.
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See the published version of this preprint at Plant Molecular Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Plant Molecular Biology.
Version 1
Posted 26 Mar, 2021
No community comments so far
Reviewers invited
Invitations sent on 23 Mar, 2021
Reviews received
Received 23 Mar, 2021
Editor invited
On 22 Mar, 2021
Editor assigned
On 20 Mar, 2021
First submitted
On 16 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Molecular Biology and Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Plant Molecular Biology.
Clematis species are commonly grown in western and Japanese gardens. Heat stress can inhibit many physiological processes mediating plant growth and development. The mechanism regulating responses to heat has been well characterized in Arabidopsis thaliana and some crops, but not in horticultural plants, including Clematis species. In this study, we found that Clematis alpina ‘Stolwijk Gold’ was heat-sensitive whereas Clematis vitalba and Clematis viticella ‘Polish Spirit’ were heat-tolerant based on the physiological analyses in heat stress. Transcriptomic profiling identified a set of heat tolerance-related genes (HTGs). Consistent with the observed phenotype in heat stress, 41.43% of the differentially expressed HTGs between heat treatment and control were down-regulated in heat-sensitive cultivar Stolwijk Gold, but only 9.80% and 20.79% of the differentially expressed HTGs in heat resistant C. vitalba and Polish Spirit, respectively. Co-expression network, protein–protein interaction network and phylogenetic analysis revealed that the genes encoding heat shock transcription factors (HSFs) and heat shock proteins (HSPs) played an essential role in Clematis resistance to heat stress. Ultimately, we proposed that two clades of HSFs may have diverse functions in regulating heat resistance from C. vitalba and CvHSFA2-2 could endow different host with high temperature resistance. This study provides first insights into the diversity of the heat response mechanisms among Clematis species.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1Supplementaryfigure1.jpg
- Additionalfile10SupplementaryTable6.xlsx
- Additionalfile11SupplementaryTable7.xlsx
- Additionalfile12SupplementaryTable8.xlsx
- Additionalfile2Supplementaryfigure2.jpg
- Additionalfile3Supplementaryfigure3.jpg
- Additionalfile4Supplementaryfigure4.jpg
- Additionalfile5SupplementaryTable1.xlsx
- Additionalfile6SupplementaryTable2.docx
- Additionalfile7SupplementaryTable3.docx
- Additionalfile8SupplementaryTable4.xlsx
- Additionalfile9SupplementaryTable5.docx
- SupplementaryInformation.docx
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