In this paper, large-scale wind power is factored into a frequency regulation model of power system. On this basis, the frequency control of the power system containing this model is investigated under potential bottleneck scenarios. Firstly, the dynamic frequency model of the power system that can reflect the impact of wind power fluctuations on grid frequency is established. Secondly, the research idea for identifying frequency regulation bottleneck scenarios in power systems is proposed, in which the frequency regulation bottleneck scenarios of power systems containing large-scale wind power are explored from the perspectives of wind power penetration rate, wind speed range, and wind speed ramping. Then, two critical bottleneck scenarios are identified: low wind speed with high wind power penetration scenario and wind power ramping in high penetration wind power systems scenario. A 3-node system is used for testing and simulation. Finally, a provincial-level empirical study in China is used to confirm its effectiveness and accuracy in addressing frequency regulation bottlenecks in the presence of large-scale wind power. It provides reference for solving the frequency control problem in large-scale wind power centralized grid connection and improving the penetration of renewable energy, as well as a scenario basis for researching and eliminating bottlenecks.