This preprint is under consideration at AMB Express. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Original article
Maria Concepcion Tamayo Ordoñez
Universidad Autonoma de Coahuila
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0201-0184
License:
This work is licensed under a CC BY 4.0 License. Read Full License
There is ongoing research related to the production of molecular hydrogen today and algae have proven to be good biological models for producing several compounds of interest. We analyzed how genetic variations in hydrogenase genes (hyd) can affect the production of molecular hydrogen in the algae Chlorella vulgaris and Scenedesmus obliquus. Through isolation and genetic characterization of hyd genes in S. obliquus and C. vulgaris, we made in-silico 3D modeling of the hydrogenase proteins and compared these in 11 algal genera. The 3D structure of hydrogenases indicated its structural conservation in 10 genera of algae, and the results of our grouping according to the aa characteristics of the proteins showed the formation of two groups, which were unrelated to the algae’s phylogenetic classification. By growing C. vulgaris and S. obliquus in anaerobic conditions (in darkness) during 24 h and after exposing the cultures to light, we observed H2 production values of 9.0 ± 0.40 mL H2/L and 16 ± 0.50 mL H2/L, respectively. The highest global relative expression of hyd genes was reached during the first 30 min of exposure to light. The behavior of the expression of the hyd genes in these species of algae proved to be species specific and involved in the production of H2. Future identification of isoforms of hyd genes in algae would allow a better understanding of the regulation of the hydrogenase enzyme.
Keywords
microalgae, hydrogenase gene, molecular hydrogen, mutation
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- FigureS1.tif
Figure S1. Phylogenetic tree of 18S of Chlorella and Scenedesmus species. Phylogeny was reconstructed by the neighbor-joining method (NJ) and cluster confidence was tested by 1000 bootstrap iterations. The amino acid sequences were aligned with Alignment Explorer/CLUSTALW program and the software Genetic and Molecular Evolution Analyses (MEGA version 6.0).
- TableS2.docx
Figure S2. In silico tertiary structures of [FeFe]-hydrogenase (Hyd) in algae. The prediction of tertiary conformation was performed using the SWISSMODEL program and Chlamydomonas reinharditti was used as a model. Predicted three-dimensional structures of A) Chamydomonas reinharditii, B) Tetradesmus, C) Tetraselmis, D) Rhapidocells subcapitata, E) Monoraphidium negletum, F) Tetraspora and G) volvox carteri f. nagariensis. The black arrows indicate the iron / sulfur cluster, the blue arrows the chloride ion and the green arrows the arsenic, important for the functionality of the enzyme.
- FigureS3.tif
Figure S3. In silico tertiary structures of [FeFe]-hydrogenase (Hyd) in algae. The prediction of tertiary conformation was performed using the SWISSMODEL program and Chlamydomonas reinharditti was used as a model. Predicted three-dimensional structures of A) Chamydomonas reinharditii, B) Coccomixa subelliposidea, c) Tetradesmus obliquus (AA65921.1), D) Tetradesmus obliqqus (CAC34419.1), E) Nannochloropsis gaditana and F) Nannochloropsis salina. The black arrows indicate the iron / sulfur cluster, the blue arrows the chloride ion and the green arrows the arsenic, important for the functionality of the enzyme.
- TableS1.docx
- figureS2.tif
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 24 Mar, 2021
No community comments so far
Reviewers invited
Invitations sent on 23 Mar, 2021
Submission checks complete
On 18 Mar, 2021
Editor invited
On 18 Mar, 2021
Editor assigned
On 17 Mar, 2021
First submitted
On 17 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Scientific Communication
Learn more about our company.
This preprint is under consideration at AMB Express. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Original article
Maria Concepcion Tamayo Ordoñez
Universidad Autonoma de Coahuila
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0201-0184
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 24 Mar, 2021
No community comments so far
Reviewers invited
Invitations sent on 23 Mar, 2021
Submission checks complete
On 18 Mar, 2021
Editor invited
On 18 Mar, 2021
Editor assigned
On 17 Mar, 2021
First submitted
On 17 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Scientific Communication
License:
This work is licensed under a CC BY 4.0 License. Read Full License
There is ongoing research related to the production of molecular hydrogen today and algae have proven to be good biological models for producing several compounds of interest. We analyzed how genetic variations in hydrogenase genes (hyd) can affect the production of molecular hydrogen in the algae Chlorella vulgaris and Scenedesmus obliquus. Through isolation and genetic characterization of hyd genes in S. obliquus and C. vulgaris, we made in-silico 3D modeling of the hydrogenase proteins and compared these in 11 algal genera. The 3D structure of hydrogenases indicated its structural conservation in 10 genera of algae, and the results of our grouping according to the aa characteristics of the proteins showed the formation of two groups, which were unrelated to the algae’s phylogenetic classification. By growing C. vulgaris and S. obliquus in anaerobic conditions (in darkness) during 24 h and after exposing the cultures to light, we observed H2 production values of 9.0 ± 0.40 mL H2/L and 16 ± 0.50 mL H2/L, respectively. The highest global relative expression of hyd genes was reached during the first 30 min of exposure to light. The behavior of the expression of the hyd genes in these species of algae proved to be species specific and involved in the production of H2. Future identification of isoforms of hyd genes in algae would allow a better understanding of the regulation of the hydrogenase enzyme.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Loading...