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Original Article
Kai-Wei Juang
National Chiayi University
Corresponding Author
License:
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Background
Genotypic variations are seen in Cd tolerance and accumulation in rice plants. Cultivars that show low Cd translocation from the root into shoot can be selected to reduce Cd contamination in rice grains. This study aims to clarify the physiological regulation related to cadmium (Cd) absorption by rice plants for screening out the cultivars, which have relatively low Cd accumulation in grains. Eight Taiwan mega cultivars of paddy rice: japonica (TY3, TK9, TNG71, and KH145 cultivars), indica (TCS10 and TCS17 cultivars), and glutinous (TKW1 and TKW3 cultivars), which are qualified with the criteria for rice grain quality by the Council of Agriculture, Taiwan, were used for illustration. An experiment in hydroponics was conducted for the rice seedlings with a treatment of 50 µM CdCl2 for 7 days.
Results and discussion
After the Cd treatment, the reductions in shoot growth were more significant than those in root growth; however, Cd absorbed in the rice plant was sequestered much more in the root. The malondialdehyde (MDA) was preferentially accumulated in rice root but the hydrogen peroxide (H2O2) was increased more significantly in the shoot; the antioxidative enzymes, superoxide dismutase (SOD) and ascorbate peroxidase (APX), were pronounced more in rice shoot.
Conclusions
The rice cultivars preferentially accumulated Cd in the root rather than the shoot with the Cd treatment, which resulted in significant enhancements of MDA and growth reductions in the root. However, H2O2 accumulation was toward the shoot to retard shoot growth suddenly and then the root could keep a gradual growth. Also, the rice cultivars, which preferentially accumulate Cd in the root, would have the regulation tendency of SOD toward the shoot. Due to that SOD is responsible for H2O2 production, H2O2 accumulation would be thus toward the shoot. Also, the cultivars, which have a less regulation tendency of APX toward the shoot, would present higher translocation of Cd into the shoot.
Keywords
Cadmium, Pollution-safe cultivar, Phytotoxicity, Abiotic stress, Antioxidant
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Peer Review Timeline
CURRENT STATUS: Published
View the published article at Botanical Studies.
No community comments so far
Editorial decision: Accept
On 28 Sep, 2020
Reviewer #1 agreed
On 27 Sep, 2020
Review #1 received
Received 27 Sep, 2020
Reviewers invited
Invitations sent on 25 Sep, 2020
Editor assigned
On 23 Sep, 2020
Submission checks complete
On 22 Sep, 2020
Editor invited
On 22 Sep, 2020
No comments provided
Review #1 received
Received 14 Sep, 2020
Editorial decision: Minor revision
On 14 Sep, 2020
Review #3 received
Received 06 Sep, 2020
Reviewer #3 agreed
On 03 Sep, 2020
Reviewer #2 agreed
On 01 Sep, 2020
Review #2 received
Received 01 Sep, 2020
Reviewers invited
Invitations sent on 31 Aug, 2020
Reviewer #1 agreed
On 31 Aug, 2020
Editor assigned
On 25 Aug, 2020
Submission checks complete
On 24 Aug, 2020
Editor invited
On 24 Aug, 2020
Version 1
Posted 15 Jun, 2020
No comments provided
Editorial decision: Major revision
On 15 Jul, 2020
Review #3 received
Received 14 Jul, 2020
Review #2 received
Received 04 Jul, 2020
Review #1 received
Received 27 Jun, 2020
Reviewer #3 agreed
On 22 Jun, 2020
Reviewer #1 agreed
On 16 Jun, 2020
Reviewer #2 agreed
On 16 Jun, 2020
Reviewers invited
Invitations sent on 15 Jun, 2020
Editor assigned
On 11 Jun, 2020
Submission checks complete
On 10 Jun, 2020
Editor invited
On 10 Jun, 2020
First submitted
On 06 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
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A new preprint design is coming!
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See the published version of this preprint at Botanical Studies.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Original Article
Kai-Wei Juang
National Chiayi University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Botanical Studies.
No community comments so far
Editorial decision: Accept
On 28 Sep, 2020
Reviewer #1 agreed
On 27 Sep, 2020
Review #1 received
Received 27 Sep, 2020
Reviewers invited
Invitations sent on 25 Sep, 2020
Editor assigned
On 23 Sep, 2020
Submission checks complete
On 22 Sep, 2020
Editor invited
On 22 Sep, 2020
No comments provided
Review #1 received
Received 14 Sep, 2020
Editorial decision: Minor revision
On 14 Sep, 2020
Review #3 received
Received 06 Sep, 2020
Reviewer #3 agreed
On 03 Sep, 2020
Reviewer #2 agreed
On 01 Sep, 2020
Review #2 received
Received 01 Sep, 2020
Reviewers invited
Invitations sent on 31 Aug, 2020
Reviewer #1 agreed
On 31 Aug, 2020
Editor assigned
On 25 Aug, 2020
Submission checks complete
On 24 Aug, 2020
Editor invited
On 24 Aug, 2020
Version 1
Posted 15 Jun, 2020
No comments provided
Editorial decision: Major revision
On 15 Jul, 2020
Review #3 received
Received 14 Jul, 2020
Review #2 received
Received 04 Jul, 2020
Review #1 received
Received 27 Jun, 2020
Reviewer #3 agreed
On 22 Jun, 2020
Reviewer #1 agreed
On 16 Jun, 2020
Reviewer #2 agreed
On 16 Jun, 2020
Reviewers invited
Invitations sent on 15 Jun, 2020
Editor assigned
On 11 Jun, 2020
Submission checks complete
On 10 Jun, 2020
Editor invited
On 10 Jun, 2020
First submitted
On 06 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Botanical Studies.
Background
Genotypic variations are seen in Cd tolerance and accumulation in rice plants. Cultivars that show low Cd translocation from the root into shoot can be selected to reduce Cd contamination in rice grains. This study aims to clarify the physiological regulation related to cadmium (Cd) absorption by rice plants for screening out the cultivars, which have relatively low Cd accumulation in grains. Eight Taiwan mega cultivars of paddy rice: japonica (TY3, TK9, TNG71, and KH145 cultivars), indica (TCS10 and TCS17 cultivars), and glutinous (TKW1 and TKW3 cultivars), which are qualified with the criteria for rice grain quality by the Council of Agriculture, Taiwan, were used for illustration. An experiment in hydroponics was conducted for the rice seedlings with a treatment of 50 µM CdCl2 for 7 days.
Results and discussion
After the Cd treatment, the reductions in shoot growth were more significant than those in root growth; however, Cd absorbed in the rice plant was sequestered much more in the root. The malondialdehyde (MDA) was preferentially accumulated in rice root but the hydrogen peroxide (H2O2) was increased more significantly in the shoot; the antioxidative enzymes, superoxide dismutase (SOD) and ascorbate peroxidase (APX), were pronounced more in rice shoot.
Conclusions
The rice cultivars preferentially accumulated Cd in the root rather than the shoot with the Cd treatment, which resulted in significant enhancements of MDA and growth reductions in the root. However, H2O2 accumulation was toward the shoot to retard shoot growth suddenly and then the root could keep a gradual growth. Also, the rice cultivars, which preferentially accumulate Cd in the root, would have the regulation tendency of SOD toward the shoot. Due to that SOD is responsible for H2O2 production, H2O2 accumulation would be thus toward the shoot. Also, the cultivars, which have a less regulation tendency of APX toward the shoot, would present higher translocation of Cd into the shoot.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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