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Research article
prahara yuri
Universitas Gadjah Mada Fakultas Kedokteran
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8403-5988
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Background : Hypospadias, the most frequent congenital male external genitalia abnormality, is usually associated with curvature of the ventral penis, i.e. chordee. Abnormality of darto tissue has been suggested as the pathophysiology of chordees. Collagen is one of the most abundant fibrous proteins within the extracellular matrix. In this study, we determined the expression of collagen 1 (COL1A1) and COL6A1 in patients with hypospadias and associated them with the severity of penile curvature. Methods : We included 60 children <18 years old, consisting of 20 distal hypospadias, 20 proximal hypospadias patients, and 20 controls in our institution from 2017 – 2020. The expression of COL1A1 and COL6A1 in darto tissue was determined by reverse-transcriptase polymerase chain reaction (qPCR). The penile curvature severity was classified as mild (<30 degrees), moderate (30-60 degrees), and severe (>60degrees). Results : qPCR showed that COL1A1 and COL6A1 expression was significantly downregulated in the distal (0.88 (0.38-2.53) and 0.54 (0.16-4.35), respectively) and proximal 0.76 (0.33-2.57) and 0.57 (0.18-1.38), respectively) hypospadias groups compared to controls (1.85 (0.24-4.61) and 0.93 (0.17-4.06), respectively) with p -values of 0.024 and 0.018, respectively. Furthermore, there was a moderate correlation between COL1A1 and COL6A1 expression ( r= 0.458, p< 0.0001). Interestingly, COL1A1 and COL6A1 were also significantly downregulated in the moderate and severe chordee groups compared to the mild chordee groups, with p -values of 0.003 and 0.037, respectively. Conclusions : Aberrant COL1A1 and COL6A1 expression might affect abnormalities in darto tissue and penile curvature severity in hypospadias patients. Keywords: chordee; COL1A1 ; COL6A1 ; expressions; hypospadias; penile curvature severity; qPCR
Keywords
chordee, COL1A1, COL6A1, expressions, hypospadias, penile curvature severity, qPCR
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CURRENT STATUS: Published
View the published article at BMC Urology.
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Editorial decision: Accept
On 18 Nov, 2020
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On 15 Nov, 2020
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On 15 Nov, 2020
Editor invited
On 15 Nov, 2020
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Editor assigned
On 29 Oct, 2020
Submission checks complete
On 29 Oct, 2020
Editor invited
On 29 Oct, 2020
Version 3
Posted 07 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 25 Oct, 2020
Review #1 received
Received 17 Oct, 2020
Reviewers invited
Invitations sent on 29 Sep, 2020
Reviewer #1 agreed
On 29 Sep, 2020
Editor assigned
On 28 Sep, 2020
Submission checks complete
On 27 Sep, 2020
Editor invited
On 27 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 20 Sep, 2020
Review #2 received
Received 18 Sep, 2020
Review #1 received
Received 14 Sep, 2020
Reviewer #2 agreed
On 30 Aug, 2020
Reviewers invited
Invitations sent on 29 Aug, 2020
Reviewer #1 agreed
On 29 Aug, 2020
Editor assigned
On 24 Aug, 2020
Submission checks complete
On 23 Aug, 2020
Editor invited
On 23 Aug, 2020
No comments provided
Editorial decision: Major revision
On 01 Aug, 2020
Review #2 received
Received 29 Jul, 2020
Reviewer #4 agreed
On 27 Jul, 2020
Review #1 received
Received 25 Jul, 2020
Reviewer #3 agreed
On 24 Jul, 2020
Reviewer #2 agreed
On 30 Jun, 2020
Reviewer #1 agreed
On 30 Jun, 2020
Reviewers invited
Invitations sent on 29 Jun, 2020
Editor assigned
On 23 Jun, 2020
Submission checks complete
On 22 Jun, 2020
Editor invited
On 22 Jun, 2020
First submitted
On 21 Jun, 2020
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Subject Areas
Urology & Nephrology
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A new preprint design is coming!
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See the published version of this preprint at BMC Urology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
prahara yuri
Universitas Gadjah Mada Fakultas Kedokteran
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8403-5988
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Urology.
No community comments so far
Editorial decision: Accept
On 18 Nov, 2020
Editor assigned
On 15 Nov, 2020
Submission checks complete
On 15 Nov, 2020
Editor invited
On 15 Nov, 2020
No comments provided
Editor assigned
On 29 Oct, 2020
Submission checks complete
On 29 Oct, 2020
Editor invited
On 29 Oct, 2020
Version 3
Posted 07 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 25 Oct, 2020
Review #1 received
Received 17 Oct, 2020
Reviewers invited
Invitations sent on 29 Sep, 2020
Reviewer #1 agreed
On 29 Sep, 2020
Editor assigned
On 28 Sep, 2020
Submission checks complete
On 27 Sep, 2020
Editor invited
On 27 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 20 Sep, 2020
Review #2 received
Received 18 Sep, 2020
Review #1 received
Received 14 Sep, 2020
Reviewer #2 agreed
On 30 Aug, 2020
Reviewers invited
Invitations sent on 29 Aug, 2020
Reviewer #1 agreed
On 29 Aug, 2020
Editor assigned
On 24 Aug, 2020
Submission checks complete
On 23 Aug, 2020
Editor invited
On 23 Aug, 2020
No comments provided
Editorial decision: Major revision
On 01 Aug, 2020
Review #2 received
Received 29 Jul, 2020
Reviewer #4 agreed
On 27 Jul, 2020
Review #1 received
Received 25 Jul, 2020
Reviewer #3 agreed
On 24 Jul, 2020
Reviewer #2 agreed
On 30 Jun, 2020
Reviewer #1 agreed
On 30 Jun, 2020
Reviewers invited
Invitations sent on 29 Jun, 2020
Editor assigned
On 23 Jun, 2020
Submission checks complete
On 22 Jun, 2020
Editor invited
On 22 Jun, 2020
First submitted
On 21 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Urology & Nephrology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Urology.
Background : Hypospadias, the most frequent congenital male external genitalia abnormality, is usually associated with curvature of the ventral penis, i.e. chordee. Abnormality of darto tissue has been suggested as the pathophysiology of chordees. Collagen is one of the most abundant fibrous proteins within the extracellular matrix. In this study, we determined the expression of collagen 1 (COL1A1) and COL6A1 in patients with hypospadias and associated them with the severity of penile curvature. Methods : We included 60 children <18 years old, consisting of 20 distal hypospadias, 20 proximal hypospadias patients, and 20 controls in our institution from 2017 – 2020. The expression of COL1A1 and COL6A1 in darto tissue was determined by reverse-transcriptase polymerase chain reaction (qPCR). The penile curvature severity was classified as mild (<30 degrees), moderate (30-60 degrees), and severe (>60degrees). Results : qPCR showed that COL1A1 and COL6A1 expression was significantly downregulated in the distal (0.88 (0.38-2.53) and 0.54 (0.16-4.35), respectively) and proximal 0.76 (0.33-2.57) and 0.57 (0.18-1.38), respectively) hypospadias groups compared to controls (1.85 (0.24-4.61) and 0.93 (0.17-4.06), respectively) with p -values of 0.024 and 0.018, respectively. Furthermore, there was a moderate correlation between COL1A1 and COL6A1 expression ( r= 0.458, p< 0.0001). Interestingly, COL1A1 and COL6A1 were also significantly downregulated in the moderate and severe chordee groups compared to the mild chordee groups, with p -values of 0.003 and 0.037, respectively. Conclusions : Aberrant COL1A1 and COL6A1 expression might affect abnormalities in darto tissue and penile curvature severity in hypospadias patients. Keywords: chordee; COL1A1 ; COL6A1 ; expressions; hypospadias; penile curvature severity; qPCR
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
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