Ado-trastuzumab for the Treatment of Metastatic HER2-Positive Breast Cancer in Patients Previously Treated with Pertuzumab

Abstract

Background: Docetaxel in combination with two HER2-directed therapies, trastuzumab and pertuzumab, is the current standard frontline therapy for patients with metastatic HER2-positive breast cancer. Ado-trastuzumab (T-DM1), an antibody-drug conjugate of trastuzumab and a cytotoxic microtubule-inhibitory agent, emtansine, is approved in patients that have progressed with prior trastuzumab-based therapy. However, the benefit of T-DM1 in patients previously treated with pertuzumab therapy for metastatic breast cancer remains unclear.

Methods: We identified thirty-three adults with metastatic HER2-positive breast cancer treated between March 2013 and July 2018 with T-DM1 either as subsequent therapy after progression on a pertuzumab-based regimen (i.e., “pertuzumab-pretreated”) or without prior exposure to pertuzumab (i.e., “pertuzumab-naïve”). Collected data included patient demographics, treatment history, adverse events, and clinical outcomes. For both cohorts receiving T-DM1, the primary endpoint was PFS and secondary endpoints were overall survival (OS), overall response rate (ORR), clinical benefit rate (CBR), and T-DM1-related toxicity rate.

Results: Pertuzumab-pretreated patients (n=23, with 21 evaluable for T-DM1 efficacy) had a median PFS of 9.5 months (95% CI: 2.9–NA), 1-year OS rate of 67.4% (95% CI: 50.0%–90.9%) with an unreached median, ORR of 14.3% (95% CI: 3.0%–36.3%), and CBR of 52.4% (95% CI: 29.8%–74.3%), with none of these measures being statistically different than those estimated for the pertuzumab-naïve group (n=10). Treatment with T-DM1 after prior pertuzumab exposure (median T-DM1 duration 2.9 months) resulted in no grade ≥ 3 adverse events.

Conclusions: Prior exposure to pertuzumab did not impact survival outcomes in patients receiving T-DM1 for metastatic HER2-positive breast cancer.

Background

The gene that encodes human epidermal growth factor receptor 2 (HER2) is amplified or overexpressed in approximately 20 percent of invasive breast cancer cases and is associated with an increased risk of disease recurrence and overall worse prognosis than HER2-negative breast cancer. The development of HER2-directed therapies has altered the natural history of HER2-positive breast cancer, and has led to a continued improvement in outcomes for this disease historically associated with a poor prognosis.

The current standard frontline therapy for patients with metastatic HER2-positive breast cancer consists of the chemotherapy agent docetaxel in combination with two HER2-directed therapies, trastuzumab and pertuzumab and is continued until disease progression or unacceptable toxicity. This standard is based on results of the CLEOPATRA trial, a phase III randomized study which demonstrated that the addition of pertuzumab to trastuzumab and docetaxel conferred both a progression-free survival (PFS) and overall survival (OS) benefit compared to trastuzumab and docetaxel alone [1, 2].

Ado-trastuzumab (T-DM1), an antibody-drug conjugate of trastuzumab and a cytotoxic microtubule-inhibitory agent, emtansine (DM1), has been studied in first-line (MARIANNE), second-line (EMILIA), and later-lines of therapy (TH3RESA) in metastatic HER2-positive breast cancer [3–5]. In all three of these trials, treatment with T-DM1 was associated with significantly improved PFS. However, these trials were conducted prior to the widespread adoption of docetaxel, trastuzumab and pertuzumab as frontline therapy. Therefore, the clinical benefit of T-DM1 in patients previously treated with pertuzumab therapy for metastatic breast cancer remains uncertain. Herein this article, we review our institution’s experience of patients treated with T-DM1 with and without prior treatment with pertuzumab for metastatic HER2-positive breast cancer.

Methods

Results

Pertuzumab-Pretreated Cohort

The cohort that received T-DM1 after progressing on a pertuzumab-containing regimen consisted of 23 women with a median age of 58 years at the time of metastatic diagnosis (range 34 to 86 years). The median time from initial breast cancer diagnosis to detection of metastases was two years (range 0 to 16 years), including 10 women diagnosed with de novo metastatic disease. The number of pre-T-DM1 systemic therapies (excluding pertuzumab) in the metastatic setting ranged from 0 (n = 6) to 8 with a median of 1. At the time of T-DM1 initiation, common sites of metastases in this cohort were the lungs (69.6% of women), bones (65.2%), liver (47.8%), and brain (43.5%). Patient-level characteristics are further summarized by treatment cohort in Table 1.

The median time between diagnosis of metastatic disease and the start of T-DM1 in this pertuzumab-pretreated group was 12.6 months (range < 1 to 70 months). The duration of T-DM1 therapy varied from 3 weeks to 4 years with a median of 2.9 months. Ten of the 23 women (43%) were given T-DM1 for more than 6 months. Patient follow-up (starting from the first T-DM1 infusion) ranged from 1 to 50 months (median 17). T-DM1-related adverse events included one patient with grade 1 cardiac dysfunction and two patients with grade ≤ 2 peripheral sensory neuropathy. Five patients (21.7%) required a T-DM1 dose reduction. Among the 21 women formally evaluated for response to T-DM1 (RECIST v1.1), there were no complete responses, 3 patients had a partial response, and 8 other patients had stable disease with > 6 months of T-DM1 treatment, leading to an overall response rate of 14.3% (95% CI: 3.0% – 36.3%) and clinical benefit rate of 52.4% (95% CI: 29.8% – 74.3%).

Pertuzumab-Naïve Cohort (Control):

The concurrent control group of HER2-positive breast cancer patients administered T-DM1 without antecedent pertuzumab in the metastatic setting consisted of 10 women, 2 of whom were diagnosed with de novo metastatic disease. With a median age of 52 years (range 38 to 67), this cohort was younger yet not statistically different than the pertuzumab-pretreated group. Pertuzumab-naive women received from 0 (n = 1) to 7 systemic therapies (median 1) between metastatic diagnosis and commencement of T-DM1.

Median duration of T-DM1 therapy was 4.8 months (range 3 weeks to 41 months). As in the pertuzumab group, T-DM1 was well tolerated, with only one out of ten patients requiring T-DM1 dose reduction. T-DM1 related adverse events included two patients experiencing cardiac dysfunction (both grade 1) and one with peripheral sensory neuropathy (grade 2). The overall response rate was 30.0% (95% CI: 6.7% – 65.2%) and clinical benefit rate was 50.0% (95% CI: 18.7% – 81.3%) based on three pertuzumab-naïve patients achieving a partial response and two others having stable disease while receiving T-DM1 for greater than 6 months.

The only patient characteristic that significantly differed between cohorts was metastasis to the lungs (p = 0.002, Table 1), which was observed in 10% of patients in the control group compared to 70% of pertuzumab-pretreated patients. All other patient features were similar across the two patient groups (p-values > 0.200, Table 1).

Survival Outcomes

Among the 23 pertuzumab-pretreated patients, the one-year PFS rate was 47.8% (95% CI: 31.2% – 73.3%) with a median PFS of 9.5 months (Fig. 1). Within this cohort, black race (HR = 4.02 [95% CI: 1.07–15.10] compared to white; p = 0.026) and liver metastasis (HR = 7.78 [95% CI: 2.07–29.26]; p < 0.001) were significantly associated with worse PFS (Table 2). Among the 10 pertuzumab-naïve patients, the 1-year PFS rate was 20.0% (95% CI: 5.8% – 69.1%), with a median PFS of 7.3 months. Thus, the pertuzumab-pretreated group had a favorable, albeit non-significant, PFS distribution compared to the pertuzumab-naïve group (HR = 0.66 [95% CI: 0.30–1.47]; p = 0.310; Table 2). Starting at 9 months after T-DM1 initiation, the proportion of patients who were alive and progression-free was greater in the pertuzumab-pretreated cohort (Fig. 1). However, 11 of the 17 PFS events in the pertuzumab group occurred before 9 months and the Prentice modification test that assigns more weight to earlier differences between groups had a p-value of 0.500 (> log-rank p-value of 0.310). Interestingly, there was a strong interaction effect on PFS between pertuzumab exposure and hepatic malignancy; pertuzumab-naïve patients with liver metastasis at the start of T-DM1 had a reduced risk of disease progression or death (HR = 0.20 [95% CI: 0.04–0.88]; p = 0.033; Table 2) compared to other control group women, which was contrary to the above mentioned greater risk of progression or death for pertuzumab-pretreated women with cancer in the liver.

There were 9 observed deaths among the 23 women in the pertuzumab-pretreated group, with deaths occurring 0.8 to 18.6 months after start of T-DM1 therapy (median 4.4 months). Median follow-up for this group was 16.9 months. The one-year OS rate was 67.4% (95% CI: 50.0% − 90.9%) and median OS was not reached (Fig. 2). None of the baseline patient demographic or disease features were associated with OS in the pertuzumab-pretreated cohort. Among the 10 pertuzumab-naïve patients, there were 9 deaths (range 5.5 to 53.1 months after starting T-DM1; median 14.0 months) and both median follow-up and median OS were 14.4 months. Patients with exposure to pertuzumab had higher 1-year (67.4% vs 60.0%) and 2-year (56.2% vs 30.0%) OS rates compared to the pertuzumab-naïve group; however, when evaluated over the entire follow-up period, this survival advantage was not statistically significant (HR = 0.56 [95% CI: 0.22–1.46]; p = 0.230; Table 3).

Compared to the respective univariable model, there was minimal change in the association between pertuzumab exposure and each time-to-event outcome when adjusting for lung metastasis (the only patient feature that significantly differed across treatment cohorts) as a covariate: PFS HR = 0.46 (95% CI: 0.17–1.20), OS HR = 0.47 (95% CI: 0.16–1.40). In regards to inferences drawn from the estimated effect of pertuzumab on survival outcomes, false negative results are an uncontrolled risk since this retrospective study was not powered to detect differences between the two treatment groups.

Discussion

This retrospective study evaluates the safety and efficacy of T-DM1 in a contemporary HER2-positive metastatic breast cancer group of patients. To our knowledge, this is the first report comparing post-T-DM1 outcomes between pertuzumab-pretreated and pertuzumab-naïve patients in the United States. This is especially important to review as T-DM1 approval was based largely on a pertuzumab-naïve population, prior to adoption of the CLEOPATRA regimen as frontline therapy for HER2-positive metastatic disease. Our results indicate that T-DM1 remains an effective and safe treatment option for patients previously treated with pertuzumab, as evidenced by a response rate, clinical benefit rate, survival distributions, and adverse reactions that were all comparable to estimates from pertuzumab-naïve patients. Although comparison of OS across the entire follow-up period did not reach statistical significance, we did observe higher 1-year and 2-year OS rates in the pertuzumab-pretreated cohort.

Our observed outcomes for patients who received pertuzumab prior to T-DM1 are consistent with most reports in the literature. Notably, our findings are similar to the overall response rate of 17.9% (95% CI: 9.4% – 26.4%) and median duration of T-DM1 of 4.0 months (95% CI: 2.7–5.1) in pertuzumab-pretreated patients as described in Dzimitrowicz et al. [6]. T-DM1 activity after progression on a regimen of trastuzumab and pertuzumab is further supported by an Italian multi-center study which demonstrated prolonged duration of therapy, defined as T-DM1 ≥ 6 months, in one-third of its patients [7]. However, a cohort of Japanese patients who received T-DM1 after progression on trastuzumab and pertuzumab had lower rates of response (11.1% vs. 25.0%) and shorter median PFS (2.8 months vs. 7.8 months) compared to a control group of pertuzumab-naïve patients [8].

Additionally, the median PFS of 9.5 months in our pertuzumab-pretreated cohort is comparable to the EMILIA trial (median PFS 9.6 months), which evaluated a similar population with a median of one prior therapy for metastatic disease, and slightly higher than the TH3RESA trial (median PFS 6.2 months), which evaluated a more heavily pretreated population compared to our cohort [3, 5]. An exploratory analysis of enrolled patients who were treated with T-DM1 after progression in CLEOPATRA and PHEREXA, two trials that assessed the benefit of adding pertuzumab to a regimen of trastuzumab and chemotherapy in the metastatic setting, provided further evidence that T-DM1 has clinical activity (median duration of therapy was 7.1 and 4.2 months in the respective trials) in patients with HER2-positive metastatic breast cancer after progression on dual HER2-directed therapy [9].

Our study also evaluated adverse events of special interest related to T-DM1 therapy; namely, cardiac dysfunction and peripheral sensory neuropathy. In doing so, we have confirmed that T-DM1 is well tolerated as there were low rates of treatment discontinuation (4% in pertuzumab-pretreated, 10% in pertuzumab-naïve) due to drug-related toxicity and no grade ≥ 3 adverse events. Furthermore, there was no significant difference in the rates of these adverse events when comparing the two treatment groups.

Prospective cooperative group-led trials attempting to determine real-world safety and efficacy of T-DM1 therapy in patients with metastatic HER2-positive breast cancer are ongoing. One such effort is the EORTC 75111 trial (NCT01597414) evaluating treatment outcomes in elderly patients with metastatic HER2-positive breast cancer [10]. In this study, a group of 29 women who received T-DM1 as a pre-determined second-line treatment option had a median PFS of 5 months. Moreover, a Swiss trial (NCT01835236) is assessing sequential treatment with trastuzumab and pertuzumab with and without chemotherapy followed by T-DM1, with overall survival at 24 months as the primary endpoint.

As the MARIANNE trial did not show benefit of T-DM1 and pertuzumab compared to T-DM1 alone, the sequential approach of delivering pertuzumab-based therapy followed by T-DM1 represents the current clinical therapy algorithm for metastatic HER2-positive breast cancer [4]. Currently, treatment with trastuzumab, pertuzumab, and a taxane followed by T-DM1 at time of disease progression has been adopted as standard-of-care first-line and second-line therapy, respectively, in patients with HER2-positive metastatic breast cancer and is endorsed by national guidelines [11–13]. Patients who progress on T-DM1 can be eligible for recently approved novel agents (Trastuzumab-deruxtecan, Tucatinib).

Conclusions

In summary, this study confirms previous reports of T-DM1 activity in patients with prior exposure to both trastuzumab and pertuzumab with a safety profile consistent with previous clinical trials. When compared to a control group of patients previously treated with trastuzumab therapy alone, there were no significant differences in clinical activity or adverse events helping to solidify T-DM1 as a second-line therapy for patients with metastatic HER2-positive breast cancer in patients previously treated with dual anti-HER2 therapy.

Declarations

Ethics approval and consent to participate: OHSU’s institutional review board’s approval and waiver of informed consent were obtained prior to commencing the chart review (OHSU IRB #18450). All methods were carried out in accordance with relevant guidelines and regulations. OHSU’s institutional review board waived the informed consent.

Consent for publication: Not applicable

Availability of data and materials: The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Competing interests: The authors declare that they have no competing interests

Funding: Not applicable

Authors' contributions: LAR, MMC, AJK collected, analyzed, and interpreted the patient data. AJK prepared Figures 1-2, Tables 1-3. MMS, MAS, and ZIM, were all major contributors in writing the manuscript. All authors read and approved the final manuscript.

Acknowledgements: Not applicable

Abbreviations

CBR: Clinical benefit rate; ER: Estrogen receptor; HR: Hazard ratio; HER2: Human epidermal growth factor receptor 2; OS: Overall survival; ORR: Overall response rate; PFS: Progression-free survival; PR: Progesterone receptor; T-DM1: Ado-trastuzumab

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