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Background
Long noncoding RNAs (lncRNAs) has long been reported to associate with multitudinous kinds of cancer. The ABHD11-AS1, a newly identified lncRNAs, has been demonstrated as a new marker of gastric cancer (GC). In this study, a significant upregulation of ABHD11-AS1 was observed in GC, compared to adjacent normal tissues.
Method
Immunohistochemical analysis for ABHD11-AS1, 3-phosphoinositide dependent protein kinase 1 (PDPK1), miR-1301-3p was performed on gastric tumor and adjacent normal tissues. The gene expression was evaluated by qRT-PCR. The proliferation was assayed by CCK8 and Annexin V/PI assay.
Results
ABHD11-AS1 is highly expressed in GC tissues compared with adjacent normal tissues(p<0.01) Knock-down of ABHD11-AS1 could suppress the aberrant proliferation of GC cells compared with si-Scramble(p<0.01). LncRNA-ABHD11-AS1 served as a sponge for miR-1301-3p in GC cell. miR-1301-3p/ABHD11-AS1 regulated the expression of PDPK1 in GC cells compared with control p<0.01). miR-1301-3p/ ABHD11-AS1 co-mediated proliferation and apoptosis of GC cell compared with si-Scramble/Mock and si-ABHD11-AS1/Mock (p<0.01)
Conclusion
PDPK1 was a downstream target of miR-133a-3p and ABHD11-AS1 subsequently exerted its biological effects via modulating the expression of PDPK1in gastric cancer cells.
Key
lncRNA,ABHD11-AS1, gastric cancer, miR-1301-3p, PDPK1
Keywords
lncRNA,ABHD11-AS1, gastric cancer, miR-1301-3p, PDPK1
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 13 Aug, 2019
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Long noncoding RNAs (lncRNAs) has long been reported to associate with multitudinous kinds of cancer. The ABHD11-AS1, a newly identified lncRNAs, has been demonstrated as a new marker of gastric cancer (GC). In this study, a significant upregulation of ABHD11-AS1 was observed in GC, compared to adjacent normal tissues.
Method
Immunohistochemical analysis for ABHD11-AS1, 3-phosphoinositide dependent protein kinase 1 (PDPK1), miR-1301-3p was performed on gastric tumor and adjacent normal tissues. The gene expression was evaluated by qRT-PCR. The proliferation was assayed by CCK8 and Annexin V/PI assay.
Results
ABHD11-AS1 is highly expressed in GC tissues compared with adjacent normal tissues(p<0.01) Knock-down of ABHD11-AS1 could suppress the aberrant proliferation of GC cells compared with si-Scramble(p<0.01). LncRNA-ABHD11-AS1 served as a sponge for miR-1301-3p in GC cell. miR-1301-3p/ABHD11-AS1 regulated the expression of PDPK1 in GC cells compared with control p<0.01). miR-1301-3p/ ABHD11-AS1 co-mediated proliferation and apoptosis of GC cell compared with si-Scramble/Mock and si-ABHD11-AS1/Mock (p<0.01)
Conclusion
PDPK1 was a downstream target of miR-133a-3p and ABHD11-AS1 subsequently exerted its biological effects via modulating the expression of PDPK1in gastric cancer cells.
Key
lncRNA,ABHD11-AS1, gastric cancer, miR-1301-3p, PDPK1
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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