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Research article
Maria Reguera
Universidad Autonoma de Madrid
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5939-8576
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Boron (B) uptake in plants implies a passive diffusion through membranes under optimal conditions. Under deficiency or toxicity, the participation of two distinct protein families (the aquaporin family Major intrinsic proteins (MIPs) and the Boron transporter family BOR) is required to minimize detrimental effects caused by B stress that ends up inhibiting growth and altering development. Legumes comprise important crops that offer major agronomic benefits including the capacity of establishing symbiosis with rhizobia fixing atmospheric N2 and for being important food protein sources. It is well proven their susceptibility to B stress leading to important yield penalties. However, little is known about the transport mechanisms responsible for B uptake and distribution in legumes, especially under deficiency.
Results: A novel legume B transporter involved in B uptake under deficiency, the Medicago truncatula homologous protein MtNIP5;1 (Medtr1g097840) to the Arabidopsis thaliana AtNIP5;1 (sharing 73,7% amino acid identity) was identified. Further analyses revealed that this M. truncatula aquaporin expression was boron-regulated in roots, being induced under deficiency and repressed under toxicity. It localizes at the plasma membrane of root epidermal cells and in nodules, where B plays pivotal roles in symbiosis. Furthermore, a partial complementation of the nip5;1 Arabidopsis mutant phenotype under B deficiency supports a functional role of MtNIP5;1 as a B transporter of in this legume model plant.
Conclusions: The results here presented support a functional role of MtNIP5;1 uptaking B under deficiency providing new insights into this micronutrient transport mechanisms in legume species.
Keywords
Boron deficiency, Medicago truncatula, aquaporins, MtNIP5;1, boron transport, legumes
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CURRENT STATUS: Published
View the published article at BMC Plant Biology.
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Submission checks complete
On 22 Nov, 2020
Editorial decision: Accept
On 19 Nov, 2020
No comments provided
Editorial decision: Minor revision
On 15 Nov, 2020
Editor assigned
On 23 Oct, 2020
Submission checks complete
On 22 Oct, 2020
Editor invited
On 22 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 20 Oct, 2020
Review #1 received
Received 28 Sep, 2020
Review #2 received
Received 28 Sep, 2020
Reviewer #2 agreed
On 25 Sep, 2020
Reviewers invited
Invitations sent on 24 Sep, 2020
Reviewer #1 agreed
On 24 Sep, 2020
Editor assigned
On 23 Sep, 2020
Editor invited
On 22 Sep, 2020
Submission checks complete
On 21 Sep, 2020
Version 1
Posted 30 Jun, 2020
No comments provided
Review #1 received
Received 23 Jul, 2020
Editorial decision: Major revision
On 23 Jul, 2020
Review #2 received
Received 13 Jul, 2020
Reviewer #2 agreed
On 09 Jul, 2020
Reviewer #1 agreed
On 06 Jul, 2020
Reviewers invited
Invitations sent on 01 Jul, 2020
Editor assigned
On 16 Jun, 2020
First submitted
On 15 Jun, 2020
Submission checks complete
On 15 Jun, 2020
Editor invited
On 15 Jun, 2020
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See the published version of this preprint at BMC Plant Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Maria Reguera
Universidad Autonoma de Madrid
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5939-8576
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Plant Biology.
No community comments so far
Submission checks complete
On 22 Nov, 2020
Editorial decision: Accept
On 19 Nov, 2020
No comments provided
Editorial decision: Minor revision
On 15 Nov, 2020
Editor assigned
On 23 Oct, 2020
Submission checks complete
On 22 Oct, 2020
Editor invited
On 22 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 20 Oct, 2020
Review #1 received
Received 28 Sep, 2020
Review #2 received
Received 28 Sep, 2020
Reviewer #2 agreed
On 25 Sep, 2020
Reviewers invited
Invitations sent on 24 Sep, 2020
Reviewer #1 agreed
On 24 Sep, 2020
Editor assigned
On 23 Sep, 2020
Editor invited
On 22 Sep, 2020
Submission checks complete
On 21 Sep, 2020
Version 1
Posted 30 Jun, 2020
No comments provided
Review #1 received
Received 23 Jul, 2020
Editorial decision: Major revision
On 23 Jul, 2020
Review #2 received
Received 13 Jul, 2020
Reviewer #2 agreed
On 09 Jul, 2020
Reviewer #1 agreed
On 06 Jul, 2020
Reviewers invited
Invitations sent on 01 Jul, 2020
Editor assigned
On 16 Jun, 2020
First submitted
On 15 Jun, 2020
Submission checks complete
On 15 Jun, 2020
Editor invited
On 15 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Plant Biology.
Background: Boron (B) uptake in plants implies a passive diffusion through membranes under optimal conditions. Under deficiency or toxicity, the participation of two distinct protein families (the aquaporin family Major intrinsic proteins (MIPs) and the Boron transporter family BOR) is required to minimize detrimental effects caused by B stress that ends up inhibiting growth and altering development. Legumes comprise important crops that offer major agronomic benefits including the capacity of establishing symbiosis with rhizobia fixing atmospheric N2 and for being important food protein sources. It is well proven their susceptibility to B stress leading to important yield penalties. However, little is known about the transport mechanisms responsible for B uptake and distribution in legumes, especially under deficiency.
Results: A novel legume B transporter involved in B uptake under deficiency, the Medicago truncatula homologous protein MtNIP5;1 (Medtr1g097840) to the Arabidopsis thaliana AtNIP5;1 (sharing 73,7% amino acid identity) was identified. Further analyses revealed that this M. truncatula aquaporin expression was boron-regulated in roots, being induced under deficiency and repressed under toxicity. It localizes at the plasma membrane of root epidermal cells and in nodules, where B plays pivotal roles in symbiosis. Furthermore, a partial complementation of the nip5;1 Arabidopsis mutant phenotype under B deficiency supports a functional role of MtNIP5;1 as a B transporter of in this legume model plant.
Conclusions: The results here presented support a functional role of MtNIP5;1 uptaking B under deficiency providing new insights into this micronutrient transport mechanisms in legume species.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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