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Research Article
Lyudmila A. Romanenko
G.B. Elyakov Pacific Institute of Bioorganic Chemisrty
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9843-3637
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A Gram-negative, non-motile bacterium КММ 3653T was isolated from a sediment sample from the Sea of Japan seashore, Russia. On the basis of the 16S rRNA gene sequence analysis the strain КММ 3653T was positioned within the family Rhodobacteraceae (class Alphaproteobacteria) forming a distinct lineage with the highest gene sequence similarities to the members of the genera Pacificibacter (95.2-94.7%) and Nioella (95.1-94.5%), respectively. According to the phylogenomic tree based on 400 conserved protein sequences, strain КММ 3653T was placed in the cluster comprising Vannielia litorea, Nioella nitratireducens, Litoreibacter albidus and Pseudoruegeria aquimaris as a separate lineage adjacent to Vannielia litorea KCTC 32083T. The average nucleotide identity values between strain КММ 3653T and Vannielia litorea KCTC 32083T, Nioella nitratireducens KCTC 32417T, Litoreibacter albidus KMM 3851T, and Pseudoruegeria aquimaris CECT 7680T were 71.1, 70.3, 69.6, and 71.0%, respectively. Strain КММ 3653T contained Q-10 as the predominant ubiquinone and C18:1ω7c as the major fatty acid followed by C16:0. The polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid, two unidentified aminolipids, and five unidentified lipids. The DNA G+C content of 61.8 % was calculated from the genome sequence. Based on the phylogenetic evidence and distinctive phenotypic characteristics, strain КММ 3653T is proposed to be classified as a novel genus and species Laetantesicola maris gen. nov., sp. nov. The type strain of Laetantesicola maris gen. nov., sp. nov. is КММ 3653T (=KCTC 82575T).
Keywords
Laetantesicola maris gen. nov., sp. nov., Alphaproteobacteia, shallow sediments, marine bacteria
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Supplementary Fig. S1. Maximum-likelihood tree based on 16S rRNA gene sequences available from the GenBank/EMBL/DDBJ databases (accession numbers are given in parentheses) showing relationships of strain KMM 3653T and related taxa. Bootstrap values based on 1000 replications are given as percentages at the branching points and numbers indicate percentages greater than 60%. Bar, 0.020 substitutions per nucleotide position. Supplementary Fig. S2. Transmission electron micrographs of bacterial cells of KMM 3653T. Bar, 1 μm. Supplementary Figure S3. Two-dimensional thin-layer chromatograms of polar lipids: a) KMM 3653T; b) Pacificibacter maritimus KMM 9031T; c) Pacificibacter aestuarii JCM 31805T; d) Vannielia litorea KCTC 32083T; e) Nioella nitratireducens KCTC 32417T; f) Nioella aestuarii JCM 30752T. Abbreviations: PC, phosphatidylcholine; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; DPG, diphosphatidylglycerol; PL, unidentified phospholipids; AL, unidentified aminolipids; L, unidentified lipids.
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This preprint is under consideration at Archives of Microbiology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Lyudmila A. Romanenko
G.B. Elyakov Pacific Institute of Bioorganic Chemisrty
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9843-3637
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 26 Mar, 2021
No community comments so far
Editor assigned
On 23 Mar, 2021
Reviewers invited
Invitations sent on 23 Mar, 2021
First submitted
On 23 Mar, 2021
License:
This work is licensed under a CC BY 4.0 License. Read Full License
A Gram-negative, non-motile bacterium КММ 3653T was isolated from a sediment sample from the Sea of Japan seashore, Russia. On the basis of the 16S rRNA gene sequence analysis the strain КММ 3653T was positioned within the family Rhodobacteraceae (class Alphaproteobacteria) forming a distinct lineage with the highest gene sequence similarities to the members of the genera Pacificibacter (95.2-94.7%) and Nioella (95.1-94.5%), respectively. According to the phylogenomic tree based on 400 conserved protein sequences, strain КММ 3653T was placed in the cluster comprising Vannielia litorea, Nioella nitratireducens, Litoreibacter albidus and Pseudoruegeria aquimaris as a separate lineage adjacent to Vannielia litorea KCTC 32083T. The average nucleotide identity values between strain КММ 3653T and Vannielia litorea KCTC 32083T, Nioella nitratireducens KCTC 32417T, Litoreibacter albidus KMM 3851T, and Pseudoruegeria aquimaris CECT 7680T were 71.1, 70.3, 69.6, and 71.0%, respectively. Strain КММ 3653T contained Q-10 as the predominant ubiquinone and C18:1ω7c as the major fatty acid followed by C16:0. The polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid, two unidentified aminolipids, and five unidentified lipids. The DNA G+C content of 61.8 % was calculated from the genome sequence. Based on the phylogenetic evidence and distinctive phenotypic characteristics, strain КММ 3653T is proposed to be classified as a novel genus and species Laetantesicola maris gen. nov., sp. nov. The type strain of Laetantesicola maris gen. nov., sp. nov. is КММ 3653T (=KCTC 82575T).
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