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Research Article
Abolghasem Ajami
Mazandaran University of Medical Sciences
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background and Objective: Innate lymphoid cells (ILCs) have been shown to play important roles in tumor immunity. We studied the frequency of three subsets of circulating ILCs in mouse models of colorectal cancer (CRC).
Methods: Two mouse models of CRC were developed; including a chemically-induced model, via administration of azoxymethane/dextran sulfate sodium (AOM/DSS), and an orthotopic mouse model, using the CT-26 cell line. Based on histopathological examinations, mice were divided into 3 groups of dysplasia group (consists of chemically-induced and orthotopic induced), chemically-induced reparative change group, normal. A sham group was also considered in which mice were screened for stresses that originated from interventions and injections. Flow cytometry analysis was performed to evaluate the frequencies of ILC1, ILC2, and ILC3 in the peripheral blood of all studied mice.
Results: The frequency of ILC1 was significantly higher in the chemically-induced reparative change group compared to the sham and dysplasia groups. ILC2s showed higher frequencies in the dysplasia groups than the sham and chemically-induced reparative change groups. In addition, altered composition of ILCs was observed in peripheral blood of dysplastic mice skewing toward ILC3s in the dysplasia groups compared to sham and chemically-induced reparative change groups.
Conclusions: A higher frequency of ILC1 in the reparative change group suggests a potentially anti-tumorigenic role. Higher ILC2s might be in favor of differentiation from the reparative change stage to the dysplasia. In addition, it seems likely that ILC3s are participating in the primary stages of CRC development.
Keywords
Innate lymphoid cell, Colorectal cancer, Orthotopically induced, chemically induced, Colitis associated colorectal cancer, peripheral blood
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Abolghasem Ajami
Mazandaran University of Medical Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 30 Mar, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background and Objective: Innate lymphoid cells (ILCs) have been shown to play important roles in tumor immunity. We studied the frequency of three subsets of circulating ILCs in mouse models of colorectal cancer (CRC).
Methods: Two mouse models of CRC were developed; including a chemically-induced model, via administration of azoxymethane/dextran sulfate sodium (AOM/DSS), and an orthotopic mouse model, using the CT-26 cell line. Based on histopathological examinations, mice were divided into 3 groups of dysplasia group (consists of chemically-induced and orthotopic induced), chemically-induced reparative change group, normal. A sham group was also considered in which mice were screened for stresses that originated from interventions and injections. Flow cytometry analysis was performed to evaluate the frequencies of ILC1, ILC2, and ILC3 in the peripheral blood of all studied mice.
Results: The frequency of ILC1 was significantly higher in the chemically-induced reparative change group compared to the sham and dysplasia groups. ILC2s showed higher frequencies in the dysplasia groups than the sham and chemically-induced reparative change groups. In addition, altered composition of ILCs was observed in peripheral blood of dysplastic mice skewing toward ILC3s in the dysplasia groups compared to sham and chemically-induced reparative change groups.
Conclusions: A higher frequency of ILC1 in the reparative change group suggests a potentially anti-tumorigenic role. Higher ILC2s might be in favor of differentiation from the reparative change stage to the dysplasia. In addition, it seems likely that ILC3s are participating in the primary stages of CRC development.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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