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Research article
Francisco R. Tadeo
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ORCiD: https://orcid.org/0000-0002-5839-4255
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Background IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like signaling peptides and the associated HAE (HAESA)-like family of receptor kinases were originally reported in the model plant Arabidopsis thaliana (Arabidopsis) to be deeply involved in the regulation of abscission. Actually, IDA peptides, as cell-to-cell communication elements, appear to be implicated in many developmental processes that rely on cell separation events, and even in the responses to abiotic stresses. However, the knowledge related to the molecular machinery regulating abscission in economically important crops is scarce. In this work, we determined the conservation and phylogeny of the IDA -like and HAE -like gene families in relevant species of the Solanaceae family and analyzed the expression of these genes in the allopolyploid Nicotiana benthamiana, in order to identify members involved in abscission, stem growth and in the response to drought conditions. Results The phylogenetic relationships among the IDA -like members of the Solanaceae studied, grouped the two pairs of NbenIDA1 and NbenIDA2 protein homeologs with the Arabidopsis prepropeptides related to abscission. Analysis of promoter regions searching for regulatory elements showed that these two pairs of homeologs contained both hormonal and drought response elements, although NbenIDA2A lacked the hormonal regulatory elements. Expression analyses showed that the pair of NbenIDA1 homeologs were upregulated during corolla abscission. NbenIDA1 and NbenIDA2 pairs showed tissue differential expression under water stress conditions, since NbenIDA1 homeologs were highly expressed in stressed leaves while NbenIDA2 homeologs, especially NbenIDA2B , were highly expressed in stressed roots. In non-stressed active growing plants, nodes and internodes were the tissues with the highest expression levels of all members of the IDA -like family and their putative HAE -like receptors. Conclusion Our results suggest that the pair of NbenIDA1 homeologs are involved in the natural process of corolla abscission while both pairs of NbenIDA1 and NbenIDA2 homeologs are implicated in the response to water stress. The data also suggest that IDA peptides may be important during stem growth and development. These results provide additional evidence that the functional module formed by IDA peptides and its receptor kinases, as defined in Arabidopsis, may also be conserved in Solanaceae.
Keywords
abiotic stress, cell separation, cis-acting regulatory elements, gene expression, LRR-RLKs, phylogeny, signaling peptide, Solanaceae, vegetative growth
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View the published article at BMC Plant Biology.
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Editorial decision: Minor revision
On 31 Dec, 2019
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On 30 Dec, 2019
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On 29 Dec, 2019
Editor invited
On 29 Dec, 2019
Version 2
Posted 25 Oct, 2019
No comments provided
Editorial decision: Minor revision
On 16 Dec, 2019
Review #2 received
Received 15 Dec, 2019
Review #1 received
Received 11 Dec, 2019
Reviewer #2 agreed
On 09 Dec, 2019
Reviewers invited
Invitations sent on 06 Dec, 2019
Reviewer #1 agreed
On 06 Dec, 2019
Editor assigned
On 21 Oct, 2019
Submission checks complete
On 20 Oct, 2019
Editor invited
On 20 Oct, 2019
No comments provided
Editorial decision: Major revision
On 16 Sep, 2019
Review #1 received
Received 14 Sep, 2019
Review #2 received
Received 11 Sep, 2019
Review #3 received
Received 11 Sep, 2019
Reviewer #3 agreed
On 01 Sep, 2019
Reviewer #2 agreed
On 29 Aug, 2019
Reviewer #1 agreed
On 27 Aug, 2019
Reviewers invited
Invitations sent on 12 Aug, 2019
Submission checks complete
On 09 Aug, 2019
Editor invited
On 09 Aug, 2019
Editor assigned
On 01 Aug, 2019
First submitted
On 30 Jul, 2019
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See the published version of this preprint at BMC Plant Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Francisco R. Tadeo
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5839-4255
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Plant Biology.
No community comments so far
Editorial decision: Minor revision
On 31 Dec, 2019
Editor assigned
On 30 Dec, 2019
Submission checks complete
On 29 Dec, 2019
Editor invited
On 29 Dec, 2019
Version 2
Posted 25 Oct, 2019
No comments provided
Editorial decision: Minor revision
On 16 Dec, 2019
Review #2 received
Received 15 Dec, 2019
Review #1 received
Received 11 Dec, 2019
Reviewer #2 agreed
On 09 Dec, 2019
Reviewers invited
Invitations sent on 06 Dec, 2019
Reviewer #1 agreed
On 06 Dec, 2019
Editor assigned
On 21 Oct, 2019
Submission checks complete
On 20 Oct, 2019
Editor invited
On 20 Oct, 2019
No comments provided
Editorial decision: Major revision
On 16 Sep, 2019
Review #1 received
Received 14 Sep, 2019
Review #2 received
Received 11 Sep, 2019
Review #3 received
Received 11 Sep, 2019
Reviewer #3 agreed
On 01 Sep, 2019
Reviewer #2 agreed
On 29 Aug, 2019
Reviewer #1 agreed
On 27 Aug, 2019
Reviewers invited
Invitations sent on 12 Aug, 2019
Submission checks complete
On 09 Aug, 2019
Editor invited
On 09 Aug, 2019
Editor assigned
On 01 Aug, 2019
First submitted
On 30 Jul, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Plant Biology.
Background IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like signaling peptides and the associated HAE (HAESA)-like family of receptor kinases were originally reported in the model plant Arabidopsis thaliana (Arabidopsis) to be deeply involved in the regulation of abscission. Actually, IDA peptides, as cell-to-cell communication elements, appear to be implicated in many developmental processes that rely on cell separation events, and even in the responses to abiotic stresses. However, the knowledge related to the molecular machinery regulating abscission in economically important crops is scarce. In this work, we determined the conservation and phylogeny of the IDA -like and HAE -like gene families in relevant species of the Solanaceae family and analyzed the expression of these genes in the allopolyploid Nicotiana benthamiana, in order to identify members involved in abscission, stem growth and in the response to drought conditions. Results The phylogenetic relationships among the IDA -like members of the Solanaceae studied, grouped the two pairs of NbenIDA1 and NbenIDA2 protein homeologs with the Arabidopsis prepropeptides related to abscission. Analysis of promoter regions searching for regulatory elements showed that these two pairs of homeologs contained both hormonal and drought response elements, although NbenIDA2A lacked the hormonal regulatory elements. Expression analyses showed that the pair of NbenIDA1 homeologs were upregulated during corolla abscission. NbenIDA1 and NbenIDA2 pairs showed tissue differential expression under water stress conditions, since NbenIDA1 homeologs were highly expressed in stressed leaves while NbenIDA2 homeologs, especially NbenIDA2B , were highly expressed in stressed roots. In non-stressed active growing plants, nodes and internodes were the tissues with the highest expression levels of all members of the IDA -like family and their putative HAE -like receptors. Conclusion Our results suggest that the pair of NbenIDA1 homeologs are involved in the natural process of corolla abscission while both pairs of NbenIDA1 and NbenIDA2 homeologs are implicated in the response to water stress. The data also suggest that IDA peptides may be important during stem growth and development. These results provide additional evidence that the functional module formed by IDA peptides and its receptor kinases, as defined in Arabidopsis, may also be conserved in Solanaceae.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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