miR-105-3p acts an oncogene to promote proliferation and metastasis of breast cancer cell by targeting GOLIM4miR-105-3p acts an oncogene to promote proliferation and metastasis of breast cancer cell by targeting GOLIM4
Background: Dysregulation of miRNAs is involved in carcinogenesis of breast and may be used as prognostic biomarkers and therapeutic targets during cancer process. The purpose of this study was to explore the effect of miR-105-3p on tumourigenicity of breast cancer and its underlying molecular mechanisms.
Methods: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was applied to detect the expression of miR-105-3p in breast cancer tissues and cell lines. The impacts of miR-105-3p on proliferation, migration, invasion and apoptosis of human breast cancer cells (MCF-7 and ZR-75-30) were evaluated by CCK-8, transwell chamber assay, TUNEL assay and western blot assay, respectively. Besides, bioinformatics and luciferase reporter assay were used to find out the target genes of miR-105-3p.
Results: The expression of miR-105-3p was elevated in breast cancer tissues and increased along with tumor severity. Downregulation of miR-105-3p could inhibit cell proliferation, suppress cell migration/invasion, and promote cell apoptosis in MCF-7 and ZR-75-30 cells. Furthermore, Golgi integral membrane protein 4 (GOLIM4) was identified to be the direct target gene of miR-105-3p by bioinformatics and luciferase reporter assay. In addition, silencing of GOLIM4 could restore the anti-breast cancer effects induced by miR-105-3p downregulation.
Conclusions: MiR-105-3p acts an oncogene to promote proliferation and metastasis of breast cancer cell by targeting GOLIM4, which provides a new target for the prevention and treatment of breast cancer.
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Posted 05 Jan, 2021
On 08 Dec, 2020
Received 02 Dec, 2020
Invitations sent on 29 Nov, 2020
On 29 Nov, 2020
On 25 Nov, 2020
On 25 Nov, 2020
On 25 Nov, 2020
On 30 Oct, 2020
Received 24 Oct, 2020
On 02 Oct, 2020
Received 01 Oct, 2020
Invitations sent on 01 Oct, 2020
On 01 Oct, 2020
On 01 Oct, 2020
Received 01 Oct, 2020
On 30 Sep, 2020
On 29 Sep, 2020
On 29 Sep, 2020
On 24 Jul, 2020
Received 22 Jul, 2020
Received 22 Jul, 2020
Received 07 Jul, 2020
On 06 Jul, 2020
On 06 Jul, 2020
Invitations sent on 02 Jul, 2020
On 02 Jul, 2020
On 25 Jun, 2020
On 24 Jun, 2020
On 24 Jun, 2020
On 24 Jun, 2020
miR-105-3p acts an oncogene to promote proliferation and metastasis of breast cancer cell by targeting GOLIM4miR-105-3p acts an oncogene to promote proliferation and metastasis of breast cancer cell by targeting GOLIM4
Posted 05 Jan, 2021
On 08 Dec, 2020
Received 02 Dec, 2020
Invitations sent on 29 Nov, 2020
On 29 Nov, 2020
On 25 Nov, 2020
On 25 Nov, 2020
On 25 Nov, 2020
On 30 Oct, 2020
Received 24 Oct, 2020
On 02 Oct, 2020
Received 01 Oct, 2020
Invitations sent on 01 Oct, 2020
On 01 Oct, 2020
On 01 Oct, 2020
Received 01 Oct, 2020
On 30 Sep, 2020
On 29 Sep, 2020
On 29 Sep, 2020
On 24 Jul, 2020
Received 22 Jul, 2020
Received 22 Jul, 2020
Received 07 Jul, 2020
On 06 Jul, 2020
On 06 Jul, 2020
Invitations sent on 02 Jul, 2020
On 02 Jul, 2020
On 25 Jun, 2020
On 24 Jun, 2020
On 24 Jun, 2020
On 24 Jun, 2020
Background: Dysregulation of miRNAs is involved in carcinogenesis of breast and may be used as prognostic biomarkers and therapeutic targets during cancer process. The purpose of this study was to explore the effect of miR-105-3p on tumourigenicity of breast cancer and its underlying molecular mechanisms.
Methods: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was applied to detect the expression of miR-105-3p in breast cancer tissues and cell lines. The impacts of miR-105-3p on proliferation, migration, invasion and apoptosis of human breast cancer cells (MCF-7 and ZR-75-30) were evaluated by CCK-8, transwell chamber assay, TUNEL assay and western blot assay, respectively. Besides, bioinformatics and luciferase reporter assay were used to find out the target genes of miR-105-3p.
Results: The expression of miR-105-3p was elevated in breast cancer tissues and increased along with tumor severity. Downregulation of miR-105-3p could inhibit cell proliferation, suppress cell migration/invasion, and promote cell apoptosis in MCF-7 and ZR-75-30 cells. Furthermore, Golgi integral membrane protein 4 (GOLIM4) was identified to be the direct target gene of miR-105-3p by bioinformatics and luciferase reporter assay. In addition, silencing of GOLIM4 could restore the anti-breast cancer effects induced by miR-105-3p downregulation.
Conclusions: MiR-105-3p acts an oncogene to promote proliferation and metastasis of breast cancer cell by targeting GOLIM4, which provides a new target for the prevention and treatment of breast cancer.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5