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Research Article
Eunice E Beaver-Kanuya
Washington State University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1771-3317
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This work is licensed under a CC BY 4.0 License. Read Full License
Tobacco ringspot virus (TRSV) is a pathogen with a wide host range that can cause disease in many perennial species. Yet detection of this virus can be insistent and unreliable, complicating disease management. In this study we asked whether seasonal effects changed the titer and distribution of the virus in Vitis vinifera, Malus domestica, and Prunus armeniaca. We developed an RT-qPCR assay and used it to monitor TRSV accumulation in leaves, stems, and roots over the course of a year. TRSV titer, and the tissues in which it accumulated, changed throughout the year in a host-specific manner which may be due to host plant growth patterns and temperature. These data will aid in determining optimal sampling time, and which tissues to collect to have confidence in diagnostic results.
Keywords
Tobacco ringspot virus (TRSV), virus detection, seasonal effects, host species
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Eunice E Beaver-Kanuya
Washington State University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1771-3317
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 20 Apr, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Virology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Tobacco ringspot virus (TRSV) is a pathogen with a wide host range that can cause disease in many perennial species. Yet detection of this virus can be insistent and unreliable, complicating disease management. In this study we asked whether seasonal effects changed the titer and distribution of the virus in Vitis vinifera, Malus domestica, and Prunus armeniaca. We developed an RT-qPCR assay and used it to monitor TRSV accumulation in leaves, stems, and roots over the course of a year. TRSV titer, and the tissues in which it accumulated, changed throughout the year in a host-specific manner which may be due to host plant growth patterns and temperature. These data will aid in determining optimal sampling time, and which tissues to collect to have confidence in diagnostic results.
Figure 1
This is a list of supplementary files associated with this preprint. Click to download.
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