High NEMO score values in nailfold videocapillaroscopy are associated with the subsequent development of ischemic digital ulcers in patients with systemic sclerosis.

doi:10.21203/rs.3.rs-37432/v4

Download PDF

Research article

High NEMO score values in nailfold videocapillaroscopy are associated with the subsequent development of ischemic digital ulcers in patients with systemic sclerosis.

https://doi.org/10.21203/rs.3.rs-37432/v4

This work is licensed under a CC BY 4.0 License

Journal Publication

published 13 Oct, 2020

Read the published version in Arthritis Research & Therapy →

You are reading this latest preprint version

Background Nailfold videocapillaroscopy (NVC) is a feasible method that allows the observation of the microvascular changes that mark the course of systemic sclerosis (SSc). In previous studies we demonstrated that the NEMO score, i.e., the cumulative number of microhaemorrhages and microthromboses, is a good indicator of the steady state level and overtime changes of disease activity (DA) in SSc.

Objectives To verify whether high NEMO scores, which mirror a very active microvascular derangement in the fingers, may be associated with the subsequent development of ischemic digital ulcers (IDUs).

Methods The NEMO score was assessed at baseline (T0) in 98 patients with SSc, all classified according to the ACR-EULAR criteria. Of them, 90 were females, 48 had the limited and 50 the diffuse cutaneous variant of SSc.

Afterwards, the patients were closely followed up for two years, and the appearance of new IDUs recorded at any time of the follow up.

The T0-NEMO score values of patients who developed IDUs were compared to those of patients who did not. A receiver operating curve (ROC) was constructed, and the area under the curve (AUC) calculated by plotting the sensitivity and 1-specificity of the different NEMO score values in predicting the subsequent development of IDUs.

Results During the follow-up 38 out of 98 patients developed one or more IDUs. The NEMO score at T0 was significantly higher in those who developed IDUs with respect to those who did not [median 14.5 (95%CI 11.0-21.5), and 4.5 (95%CI 4.0-6.0), respectively, p<.0001]. The ROC curve derived from different T0-NEMO score values had an AUC of 0.79 (95%CI 0.69-0.86, p<0.0001)]. A NEMO score of ≥12 had a sensitivity of 83.3% (95%CI 71.5-91.7), and a specificity of 63.2% (95%CI 46.0-78.2), with positive (P) and negative (N) predictive (PV) values of 58.9% (95%CI 44.7-72.2), and 85.6% (71.8-94.4), respectively. A NEMO score of ≥16 had a sensitivity of 95.0% (95%CI 86.1-99.0), and a NPV of 93.4% (77.5-99.2).

Conclusions Being a valid tool to measure DA levels in SSc, the NEMO score also appears to be closely related to the subsequent development of IDUs in this disease.

Rheumatology

Medical Genetics

systemic sclerosis

ischemic digital ulcers

nailfold videocapillaroscopy

Nailfold videocapillaroscopy (NVC) is a valid, feasible and non-invasive method to observe the abnormalities of the microvascular bed in different pathological conditions (1). Specific features observed with this methodology have been found in some connective tissue diseases, namely in systemic sclerosis (SSc) (1-3). Therefore, NVC has been considered a good tool to ascertain the diagnosis of SSc, and has been included among the items composing the most recent classification criteria for this disease, defined by the American College of Rheumatology (ACR) and European League Against Rheumatism (EULAR) (4).

Furthermore, progressive changes of NVC features have been observed during the clinical course of SSc and, as consequence of this, different NVC patterns has been described in early, active and late phases of the disease (5). According to these findings, the active phase of SSc is characterized by the prevalent presence of ectasic and giant capillaries, microhaemorrhages (MHEs) and microthromboses (MTs).

During the last few years the NEMO score, i.e., the cumulative number of MHEs and MTs, has been proposed and validated as an good tool to assess both steady state (6,7) and overtime changes (8) of disease activity (DA) in the course of SSc. The NEMO score, in fact, showed a very close correlation with the composite scales which have been defined to assess this disease status entity, as those proposed by the European Scleroderma Study Group (EScSG) (9,10) and, subsequently, by the European Scleroderma Clinical Trials and Research (EUSTAR) (11).

Since a more extensive involvement of microvascular bed in the active phase of SSc, which is correlated to highest values of the NEMO score, may evolve to a more severe capillary loss, and distal ischemic damage, in the present study we have investigated whether the highest levels of the NEMO score may be associated with the subsequent development of ischemic digital ulcers (IDUs).

Patients

The study was carried out in the cohort of patients with SSc that was used in the validation study of NEMO score. This cohort was initially composed of 102 patients with SSc who were referred to the Scleroderma Clinics of the Rheumatic Disease Unit of the Gaetano Pini Institute of Milan. Since four of these patients dropped out from the follow-up, the present study was completed in 98 patients.

All enrolled patients met the ACR/EULAR classification criteria for SSc (4), and they were also sub-classified as having limited cutaneous SSc (lcSSc) or diffuse cutaneous SSc (dcSSc) according to the LeRoy et al. criteria (12). At the time of the enrolment, it was preliminarily established to include around half the patients with inactive disease (EScSG score < 3) and a similar proportion of

patients with active disease (EScSG score ≥ 3) (7).

Exclusion criteria were pre-existing conditions that may induce additional microvascular changes, such as diabetes, smoking and onychophagic habitus, presence of anti-phospholipid antibodies, and pregnancy (13-16). Another exclusion criterion was current treatment with beta-blockers which may exacerbate Raynaud’s phenomenon (RP) (17).

At the time of study enrolment, all of the patients were receiving low-dose acetylsalicylic acid and calcium channel blockers (CCBs), and in addition around one third of the patients were on treatment with other vasoactive agents (31 with monthly infusion of iloprost, 5 with weekly infusion of alprostadil, 7 with oral bosentan and 4 with sildenafil). None of the patients was taking anti-coagulant therapy. Three out of these latter patients had pulmonary hypertension. For ethical reasons these vasoactive therapies were maintained throughout the study.

Nailfold videocapillaroscopy

NVC was performed in all the patients at enrolment time (T0) by using a videocapillaroscope with a x 200 magnification lens. All fingers of both hands, excluding thumbs, of each patient were examined by positioning each digit in such a way that the capillaroscopic light was 90 degrees incident on the centre of the nailfold. Four adjoining 1-mm fields—two on the right and two on the left side, starting from the middle of the nailfold and for a total extension of 4 mm—were examined (6,7). The derived digital images were stored using dedicated software (VideoCap; Scalar Co. Ltd., Tokyo, Japan). One experienced investigator (FP) was responsible for reviewing and scoring the stored NVC images of all the study patients, according to the NEMO definition (8).

Assessment of digital ulcers

All the enrolled patients were carefully observed every three months for the following two years, with particular attention given to the new appearance of at least one IDU in the distal fingers. A IDU was defined as a painful area, of at least 6 mm in diameter at its longest point, with visible depth and loss of dermis, located at the volar surface of the digit, distal to the proximal interphalangeal digital crease.

Statistical analysis

Statistical analysis was performed using MedCalc software package, 2014 version (MedCalc® Inc., Ostend, Belgium).

The Mann Whitney test was applied to compare T0-NEMO score values recorded in patients who subsequently developed IDUs to those assessed in patients who did not. The T0-NEMO score values were also analysed in sub-groups of patients subdivided according their NVC patterns and SSc cutaneous variants. This non-parametric method was adopted because the NEMO score variable did not have a normal distribution (Shapiro-Wilk test, p > 0.05).

Other variables as disease duration, NVC patterns, SSc cutaneous variants, autoantibody specificities, history of previous ulcers and types of vasoactive therapies, were also tested for a possible association with the subsequent development of IDUs, using chi-square cross tabs for categorical variables and Mann-Whitney test for discrete variables.

Receiver operating characteristic (ROC) curves were constructed by plotting the sensitivity and 1-specificity values of the T0-NEMO scores in identifying patients who developed IDUs during the following two-year observation time. The area under the curve (AUC) was calculated together with the related 95% confidence intervals (CI) by applying the Hanley-McNeil test.

We also identified the sensitivity, specificity, positive (P) and negative (N) predictive values (PV) of the different T0-NEMO scores with the best performance in capturing patients who developed IDUs in the subsequent follow-up observation.

The Kaplan-Meier curve analysis and log rank test were used to evaluate the occurrence and the time of the appearance of IDUs during the follow-up in patients having different levels of T0- NEMO score. Hazard ratio (HR) with the related 95% CI were calculated and used for the comparison between the two curves.

Finally, a logistic regression model was tested in which the appearance of new digital ulcers in the follow-up represented the independent variable, while dependent variables were those that showed to be separately associated with the development of IDUs.

The clinical characteristics of the patients included in this study are reported in table 1.

The cohort was composed of 98 patients classified as having SSc according to the ACR/EULAR criteria (4). They were aged between 21 and 84 years, with a large prevalence of females (ratio 10/1), and a comparable number of patients having lcSSc and dcSSc (48 and 50, respectively). The NVC pattern (5) was defined as early, active and late in 16, 42 and 40 patients, respectively. Twenty four out of 98 patients experienced one of more IDUs before their enrolment in the study. Anti-centromere and anti-topoisomerase-I antibodies were positive in 42 and 50 patients respectively.

During the follow-up 38 out of 98 patients (38.8%) developed one or more new IDUs. The T0-NEMO scores were significantly more elevated in the patients who developed IDUs with respect to those who did not [median 14.5 (95% CI 11.0-21.5), and 4.5 (95% CI 4.0-6.0), respectively, p<.0001] (Figure 1a). As expected, the T0-NEMO scores were significantly higher in patients with NVC active pattern [median 13.0 (95% CI 10.4-16.8)] with respect to those with early NVC pattern [median 5.5 (95% CI 1.6-12.4), p<0.02], and late NVC pattern [median 4.0 (95% CI 1.0-6.0), p< 0.0001]. No difference of the T0-NEMO score values was recorded between patients with early and late NVC patterns (p=0.23). Finally, the T0-NEMO scores were significantly more elevated in patients with dcSSc in comparison with patients with lcSSc [median 12.0 (95% CI 9.0-15.0) vs 5.0 (95% CI 4.0-6-0), p<0,003].

The appearance of new IDUs was associated with the history of previous ulcers, and with the presence of the diffuse cutaneous variant of the disorder (Chi square 15.6, p<0.0001 and 7.8, p<0.01, respectively). A similar association was found with the presence of anti-topoisomerasi I antibodies that are notoriously closely related with the dcSSc variant of the disorder. Conversely, no association was found between the appearance of IDUs and disease duration or use of more aggressive vasoactive therapies (monthly iloprost infusion, oral bosentan or sildenafil) in addition to basal CCBs. IDUs development during the follow up was observed in 4/16 (25.0%), 20/42 (47.6%), and 14/40 (35.0%) patients with early, active, and late NVC pattern, respectively. No statistically significant difference of the IDU prevalence between these NVC groups was recorded.

A logistic regression model in which all the three variables independently linked with subsequent appearance of IDUs were taken into account (T0-NEMO score, history of previous IDUs, and dcSSc variant) showed that only the history of previous ulcers and T0-NEMO gave a significant contribution to the model, whilst the third variable was discharged. (see table 2).

The ROC curve obtained by plotting sensitivity and 1-specificity of the different T0-NEMO score values in identifying patients who developed IDUs is represented in Figure 2a. The AUC of this ROC curve was 0.79 (95% CI 0.69-0.86, p<0.0001).

A NEMO score of 12 or more showed a sensitivity of 83.3% (95% CI 71.5-91.7), and a specificity of 63.2% (46.0-78.2), with a PPV and NPV of 58.9% (95% CI 44.7-72.2), and 85.6% (71.8-94.4), respectively. Furthermore, a NEMO score of 16 or more was highly predictive of future development of IDUs showing, in this respect, a sensitivity of 95.0% (95% CI 86.1-99.0), and a NPV of 93.4% (95% CI 77.5-99.2).

The Kaplan-Meier curve analysis confirmed that the development of subsequent IDUs was significantly more frequent in patients having a T0-NEMO score of 12 or more with respect to those with a lower baseline NEMO score. The difference between the two curves became significant after 6 months from T0 (Figure 3a).

When the same statistical analysis was limited to the 74 patients who had no previous occurrence of IDUs, the results were very similar. Twenty-eight of them (37.8%) developed at least one IDU during the two-year follow-up. The T0-NEMO scores had a median value of 14.0 (95% CI 10.8-22.9) in the patients who developed IDUs, and of 4.5 (95% CI 3.9-6.3) in those who did not (p<0.0001] (Figure 1b). The AUC of the ROC curve (Figure 2b) was slightly higher [0.86 (95% CI 0.76-0.93), p<0.0001] than that found taking into consideration the totality of patients. This difference, however, was not significant (z value 1.01, p=0.3). In this more restricted cohort a NEMO score of 12 or more had a sensitivity of 80.4% (95%CI 66.1-90.6), and a specificity of 78.6% (95% CI 59.0-91.7), while a NEMO score of 16 or more maintained a strongly predictive value of future development of IDUs showing a sensitivity 93.5% (95%CI 82.1-98.6), and NPV of 93.1% (95% CI 76.2-99.3).

A Kaplan-Meyer curve was also built and analyzed in patients who were naïve for previous IDUs. Even in this case, the appearance of IDUs was more frequent in patients having a T0-NEMO score ≥12, and this difference was comparably significant at 6 and 24 months (Figure 3b).

The statistical analysis was also performed in patients with early and active NVC pattern, thus excluding those patients with late NVC pattern who had more pronounced fibrotic changes.

In this selected cohort the T0-NEMO scores had a median value of 24.0 (95% CI 15.0-34.5) in patients who developed IDUs and of 5.5 (95%CI 4.0-10.3) in those who did not (p<0.0001) (Figure 1c). The AUC of the ROC curve (Figure 2c) was again slightly higher [0.89 (95% CI 0.78-0.96), but not significantly different from that obtained in the whole group of patients (z value 1.45, p=0.14). A NEMO score of 16 or more was again very strongly associated with future development of IDUs, showing a sensitivity of 94.1 (95%CI 82.4-97.4) and a NPV of 94.1 (95%CI 76.4-99.6).

In previous studies we demonstrated that the NEMO score, computed during NVC examination, is a good tool to measure both steady state and overtime changes of DA in SSc (6-8), being strictly correlated with the multi-item scales proposed to measure the same disease status entity (9-11).

In this study we demonstrate that high values of the NEMO score are also associated with the future development of IDUs in patients with SSc. Patients with a NEMO score of 16 or more have a probability of incidental IDUs of around 95% in the subsequent two years.

When the ROC curve analysis was carried out, the performance of the NEMO score as indicator of future development of IDUs is slightly higher, although not significantly different, when the analysis was limited to patients who were naïve for previous occurrence of IDUs and to those with early and active NVC patterns. The present results indicate that high levels of NEMO score are able to forecast the future development of IDUs independently of the fact that the patients experienced or not previous ulcers. However, the present data confirm that the history of previous ulcers is associated with the following development of new ulcers.

In a previous study, Sebastiani et al. (18) demonstrated that the development of IDUs in the following three months was associated with a low number of capillaries and a high number of megacapillaries in NVC. These data are not contradictory with respect to the present ones. It is plausible that a late pattern of NVC is able to forecast the development of IDUs in the subsequent short time, whilst high NEMO score values are related with a later appearance of IDUs as showed by the survival curve in the present study (Figure 3). This statement is supported by the present knowledge on the evolution of microvascular lesions in SSc. Endothelial damage is considered the first step of the evolving pathological process in this disease (19-21), and in the later phase the destiny of many capillaries is thrombotic obliteration followed by extravasation. Therefore, multiple MTs and MHEs which are observed in NVC, aligned distally in the cuticle, can be the mirror of the synchronous pathological aggression of many capillaries (22). In this view, the NEMO score can be considered as a relatively simple method to quantify this phenomenon.

The subsequent response to the initial microvascular damage in SSc is the loss of capillaries. The compensatory dilatation of residual capillary loops with formation of enlarged capillaries and GCs is believed as an ineffective tentative process of capillary regeneration (23). The final step of the process is a sort of capillary desertification accompanied by fibrotic changes. On the basis of this sequence of pathological events, one can presume that the higher the NEMO score values, the greater the possibility is that the following fibrotic and ischemic changes will be more severe and rapid. Thus, it is not surprising that the highest NEMO score values can anticipate the subsequent development of IDUs.

The occurrence of IDUs on the fingertips which has been observed in around half of the patients with SSc (24-26), commonly leads to a significant worsening of the patient’s quality of life often causing often severe pain and difficulties in performing the simplest daily living activities. The healing of IDUs is frequently a lengthy process requiring accurate and intensive topical and systemic treatment [25].

Therapeutic measures, aimed at avoiding or limiting the development of IDUs in patients with SSc, usually consist of the use of vasoactive agents such as CCBs, prostanoids, endothelin receptor antagonists and phosphodiesterasis-5 inhibitors. For all these agents, there is no evidence of effectiveness in the primary prevention of IDUs development, whilst several studies have demonstrated their beneficial effects in preventing new IDUs and in favoring the healing of active ulcers (27,28).

The present study shows that slightly less than 40% of the patients who were naïve for previous IDUs developed this kind of lesions in the follow up, and in a significant proportion of them this happens after few months. One can postulate that a more aggressive vasoactive therapy could be reserved to patients with very high NEMO score values (for instance those with 16 or more NEMO score values at baseline). These patients have around 95% of probability of future occurrence of IDUs, even in a relatively short time.

This is a retrospective study in a relatively limited cohort of patients collected in an unique centre. The results of this study need to be confirmed in a prospective multicentre study that should include a larger cohort of SSc patients

The NEMO score, which has been previously proposed as valid tool to measure DA in SSc, demonstrated that it is also able to identify patients who, having the highest values of this NVC index, are candidates for the subsequent development of IDUs. The identification of this subset of patients suggests that more aggressive therapies could be reserved to them to prevent the subsequent appearance of such a painful and life-threatening complication.

ACA: Anti-centromere antibodies; ACR/EULAR: American College of Rheumatology/European League Against Rheumatism; AUC: area under the curve; CCBs: calcium channel blockers; CI: confidence interval; DA: disease activity; dcSSc: diffuse cutaneous systemic sclerosis; EScSG: European Scleroderma Study Group; EUSTAR: European Scleroderma Trial and Research; GCs: giant capillaries; IDUs: ischemic digital ulcers; lcSSc: limited cutaneous systemic sclerosis; MHEs: micro-haemorrhages; MTs: micro-thrombosis; NEMO: Number of micro-haemorrhages and micro-thromboses; NPV: negative predictive values; NVC: nailfold videocapillaroscopy; PPV: positive predictive values; ROC: receiver operating characteristic; SSc: systemic sclerosis.

Ethical approval and consent to participate

This study was approved by the ethics committee of the ‘Azienda Socio Sanitaria Territoriale Lombardia, Centro Specialistico Ortopedico Traumatologico Gaetano Pini ‘, Milan, where the study was carried out. Written informed consent was obtained from all of the enrolled

patients.

Consent for publication

All the authors give their consent for publication of the study.

Written informed consent was obtained from the patients for publication of their anonymised data published in this article. No identifiable patient data are contained in this article.

Availability of data and materials

The datasets used and analyses made during the current study are available from the corresponding author on reasonable request.

Competing interest

The authors declare that they have no competing interests.

Funding

This research did not receive any funding.

Authors’ contribution

FP, AM, DS and GS made substantial contributions to the acquisition of capillaroscopic data. AM, FP, WM, and NDP made substantial contributions to the acquisition of clinical data. DS and GS contributed to study conception and design. GV, CV, RC and NDP made substantial intellectual contributions to study conception and data interpretation, and they helped draft and revise the manuscript for important intellectual content. FP, WM, AM, DS, GS, GV, CV, RC and NDP agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. FP, WM, AM, DS, GS, GV, CV, RC and NDP read and approved the final manuscript.

Acknowledgements

We are grateful to Ms Wendy Doherty for revising the English in the manuscript.

Cutolo M, Pizzorni C, Secchi ME, Sulli A. Capillaroscopy. Best Pract Res Clin Rheumatol 2008,22:1093-108.
Ingegnoli F, Boracchi P, Gualtierotti R, Lubatti C, Meani L, Zahalkova L, et al. Prognostic model based on nailfold capillaroscopy for identifying Raynaud’s phenomenon patients at high risk for the development of a scleroderma spectrum disorder. Arthritis Rheumatol 2008,58:2174-82.
Sulli A, Secchi ME, Pizzorni C, Cutolo M. Scoring the nailfold microvascular changes during the capillaroscopic analysis in systemic sclerosis patients. Ann Rheum Dis 2008,67:885–7.
Van den Hoogen F, Khanna D, Fransen J, Johnson SR, Baron M, Tyndall A, et al: 2013 Classification criteria for systemic sclerosis. An American College of Rheumatology/European League Against Rheumatism Collaborative Initiative. Arthritis Rheumatol 2013,65:2737-47.
Cutolo M, Sulli A, Pizzorni C, Accardo S. Nailfold videocapillaroscopy assessment of microvascular damage in systemic sclerosis. J Rheumatol 2000;27:155-60.
Sambataro D, Sambataro G, Zaccara E, Maglione W, Polosa R, Afeltra AM, e al. Nailfold videocapillaroscopy micro-haemorrhage and giant capillary counting as an accurate approach for a steady state definition of disease activity in systemic sclerosis. Arthritis Res Ther 2014;16:462-69.
Andracco R, Irace R, Zaccara E, Vettori S, Maglione W, Riccardi A, et al. The cumulative number of micro-haemorrhages and micro-thromboses in nailfold videocapillaroscopy is a good indicator of disease activity in systemic sclerosis: a validation study of the NEMO score. Arthritis Res Ther 2017;19:133-41.
Pignataro F, Maglione W, Minniti A, Sambataro D, Sambataro G, Campanaro F, et al. NEMO score in nailfold videocapillaroscopy is a good tool to assess both steady state levels and overtime changes of disease activity in patients with systemic sclerosis: a comparison with the proposed composite indices for this disease status entity. Arthritis Res Ther. 2019 21:258-64.
Valentini G, Della Rossa A, Bombardieri S, Bencivelli W, Silman AJ, D'Angelo S, et al. European multicentre study to define disease activity criteria for systemic sclerosis. II. Identification of disease activity variables and development of preliminary activity indexes. Ann Rheum Dis 2001;60:592-8.
Valentini G, Bencivelli W, Bombardieri S, D’Angelo S, Della Rossa A, Silman AJ, et al. European multicenter study to define disease activity criteria for systemic sclerosis. III. Assessment of the construct validity of the preliminary activity criteria. Ann Rheum Dis 2003; 62:901-3
Valentini G, Iudici M, Walker UA, Jaeger VK, Baron M, Carreira P, et al. The European Scleroderma Trials and Research group (EUSTAR) task force for the development of revised activity criteria for systemic sclerosis: derivation and validation of a preliminarily revised EUSTAR activity index. Ann Rheum Dis 2017;76:270-6.
LeRoy EC, Black C, Fleischmajer R, Jablonska S, Krieg T, Medsger TA Jr, et al. Scleroderma (systemic sclerosis): classification, subsets and pathogenesis. J Rheumatol 1988,15:202–5.
Maldonado G, Guerrero R, Paredes C, Ríos C. Nailfold capillaroscopy in diabetes mellitus. Microvasc Res. 2017;112:41-6.
Yuksel EP, Yuksel S, Soylu K, Aydin F. Microvascular abnormalities in asymptomatic chronic smokers: a videocapillaroscopic study. Microvasc Res. 2019;124:51-3.
Vaz JL, Dancour MA, Bottino DA, Bouskela E. Nailfold videocapillaroscopy in primary antiphospholipid syndrome (PAPS). Rheumatology (Oxford). 2004; 43:1025-7
Thevissen K, Gyselaers W. Capillaroscopy in pregnancy. Expert Rev Med Devices. 2017;14(12):961-7.
Garner R, Kumari R, Lanyon P, Doherty M, Zhang W. Prevalence, risk factors and associations of primary Raynaud’s phenomenon: systematic review and meta-analysis of observational studies. BMJ Open. 2015;5(3):e006389.
Sebastiani M, Manfredi A, Colaci M, et al. Capillaroscopic skin ulcer risk index: a new prognostic tool for digital skin ulcer development in systemic sclerosis patients. Arthritis Rheum. 2009;61(5):688-694. doi:10.1002/art.24394
Kahaleh B: The microvascular endothelium in scleroderma. Rheumatology (Oxford) 2008; 47:14-15.
Gabrielli A, Avvendimento EV, Krieg T. Scleroderma. N Eng J Med 2009;360:1989–2003.
Del Papa N, Colombo G, Fracchiolla N, Mazzeo Moronetti L, Ingegnoli F, Maglione W, et al. Circulatin endothelial cells as a marker of ongoing vascular disease in systemic sclerosis. Arthritis Rheumatol 2004;50:1296-304.
Wong ML, Highton J, Palmer DG. Sequential nailfold capillary microscopy in scleroderma and related disorders. Ann Rheum Dis 1988;47:53-61.
Cutolo M, Sulli A, Smith V. How to perform and interpret capillaroscopy. Best Pract Res Clin Rheumatol. 2013;27:237-48.
Ferri C, Valentini G, Cozzi F, Sebastiani M, Michelassi C, La Montagna, G, et al. Systemic sclerosis: Demographic, clinical, and serologic features and survival in 1,012 Italian patients. Medicine (Baltimore) 2002; 81:139-53.
Steen V, Denton CP, Pope JE, Matucci-Cerinic M. Digital ulcers: overt vascular disease in systemic sclerosis. Rheumatology 2009; 48(Suppl 3):19-24.
Walker UA, Tyndall A, Czirják L, Denton C, Farge-Bancel D, Kowal-Bielecka O, et al. Clinical risk assessment of organ manifestations systemic sclerosis: A report from the EULAR Scleroderma Trials and Research Group database. Ann Rheum Dis 2007; 66:754-63.
Kowal-Bieleka O, Fransen J, Avouac J, Becker M, Kulak A, Allanore Y, et al. Update of EULAR recommendations for the treatment of systemic sclerosis. Ann Rheum Dis 2017;76:1327-39.
Tingey T, Shu J, Smuczek J, Pope J. Meta-analysis of healing and prevention of digital ulcers in systemic sclerosis. Arthritis Care Res 2013;65:1460-71.

Table 1. Demographic and clinical characteristics of the cohort of patients with SSc enrolled in the study
Numbers of patients	98
Male/female	8/90
Median age, years (range)	58 (21-84)
Median disease duration, years (range)	6 (0-26)
lcSSc/dcSSc	48/50
Autoantibodies
ACA, n (%)	42 (42.8)
Anti-topoisomerase 1, n (%)	50 (51)
Others, n (%)	6 (6.1)
NVC patterns
Early, n (%)	16 (16.2)
Active, n (%)	42 (42.8)
Late, n (%)	40 (41)
Patients on prostanoid therapy, n (%)	36 (36.7)
Patients on Bosentan/Sildenafil therapy, n (%)	11 (11.2)
Legend: dcSSc, diffuse cutaneous systemic sclerosis; lcSSc, limited cutaneous systemic sclerosis; ACA, Anti-centromere antibody; NVC, nailfold videocapillaroscopy.

Table 2. Logistic regression model in which the subsequent appearance of IDUs was the dependent variable and the NEMO score values, history of previous IDUs, and the presence of dcSSc variant represented the independent variables.

Total number of patients with SSc- 98

Number of patients who developed new IDUs- 38

Number of patients who did not develop a new IDU- 60

Independent variables coefficient (standard error) p

T0-NEMO score 0.12 (0.03) =0.0001

Previous IDUs 2.63 (0.63) <0.0001

dcSSc variant discharged -

Constant -2.52

Full model likelihood chi square p

98.32 44.56 <0.0001

Download PDF

Journal Publication

published 13 Oct, 2020

Read the published version in Arthritis Research & Therapy →

Editorial decision: Accept
03 Oct, 2020
Reviewers invited by journal
29 Sep, 2020
Reviewer #1 agreed at journal
29 Sep, 2020
Review #1 received at journal
29 Sep, 2020
Editor assigned by journal
28 Sep, 2020
Submission checks completed at journal
27 Sep, 2020
Editor invited by journal
27 Sep, 2020

You are reading this latest preprint version

High NEMO score values in nailfold videocapillaroscopy are associated with the subsequent development of ischemic digital ulcers in patients with systemic sclerosis.

Status:

Journal Publication

Version 4

Abstract

Figures

Introduction

Patients And Methods

Results

Discussion

Conclusions

Abbreviations

Declarations

References

Tables

Status:

Journal Publication

Version 4