The pseudo-Jahn-Teller (PJT) effect, a direct indicator of molecular instability, can be the reason of sub-meV split degenerate electronic states that can be used to discriminate enantiomers. In the exemplar chiral molecule of camphor (C10H16O), the nondegenerate vibrational states were previously identified, but there has been no evidence of PJT states. Here, linearly polarized Raman measurements were employed to demonstrate the PJT states, with splitting of 1.8 ± 0.2 cm-1, for scattering by chiral centers on carbon ring of camphor. This observation is attributed to the symmetry reduction and chirality-dependent C3 symmetry becoming intact by placing camphor on a SiO2/Si substrate. The depolarization ratio, the noncoincidence effect, and the molecular chirality-dependent polarization rotation of the PJT states manifested a clear distinction between enantiomers. More importantly, quantification of chirality was possible based on the occupancy of the PJT states at the depolarized state, which is applicable to similar chiral molecules.