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Leopoldo Santos Argumedo
CINVESTAV IPN: Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional
Corresponding Author
ORCiD: https://orcid.org/0000-0002-4772-0713
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: Microbiota colonization during labor and through the first meals contributes to immune maturation and development of the newborn. Mother provides probiotics and prebiotics factors through colostrum and maternal milk to shape the first neonatal microbiota. Previous works have reported that immunoglobulin A (IgA) secreted in colostrum is coating a fraction of maternal microbiota.
Methods: Thus, to better characterize the IgA-associated microbiota, we used flow cytometry coupled with 16S rDNA gene sequencing (IgA-Seq) in human colostrum and neonatal feces. We identified IgA-bound bacteria (IgA+) and characterized its diversity to elucidate possible role of IgA subclasses during neonatal bacterial colonization of the colon.
Results: We found that IgA2 in the colostrum has an active role during microbiota colonization. Colostrum IgA2 is mainly associated with Bifidobacterium, Lactobacillus, and Bacteroidetes genera. This association seems to give these bacteria an advantage during their establishment since metabolic pathways related to epithelial adhesion and carbohydrate consumption are enriched within fecal IgA2+ microbiota. Association with specific bacteria could be explained since IgA2 recognizes common antigens expressed on surfaces among bacteria genera.
Conclusions: Our data suggest a specific targeting of commensal bacteria by IgA2 revealing a specialized function of IgA microbiota association during neonatal intestinal colonization during the first days of life.
Keywords
IgA2, IgA1, Microbiota, Colostrum, Meconium, Maternal transfer, IgA-seq
Figure 1
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Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- Supplementary1.pptx
Supplementary 1. Bacterial proteases preferentially degrade IgA1. Kinetical evaluation of the stability of IgA1 (left) and IgA2 1 (right), in two different conditions of temperature and presence/absence of protease inhibitors, in contact with IgA2+ or IgA1+ 2 bacteria fractions from colostrum; in minutes (min); by ELISA. All values are represented as mean ± SD. All data are expressed 3 in milligrams of IgA subclasses per milliliter (mg/mL). Statistical analysis was performed using the Pearson correlation test. *p 4 <0.05, **p <0.01 and ***p <0.001. 5
- Supplementary2.pptx
Supplementary 2. Some of the newborn fecal microbiota have their origin in the colostrum. (A) Microbial source tracker analysis 6 showing the proportion of bacteria identified in the neonatal stool classified by source (p < 0:001, Wilcoxon signed-rank test). 7 (B) The relative abundance of most common bacterial orders found in the neonatal stool is classified by Qiime source tracker 8 analysis as "Other sources" and "Colostrum source".
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Leopoldo Santos Argumedo
CINVESTAV IPN: Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional
Corresponding Author
ORCiD: https://orcid.org/0000-0002-4772-0713
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 06 Apr, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Microbiota colonization during labor and through the first meals contributes to immune maturation and development of the newborn. Mother provides probiotics and prebiotics factors through colostrum and maternal milk to shape the first neonatal microbiota. Previous works have reported that immunoglobulin A (IgA) secreted in colostrum is coating a fraction of maternal microbiota.
Methods: Thus, to better characterize the IgA-associated microbiota, we used flow cytometry coupled with 16S rDNA gene sequencing (IgA-Seq) in human colostrum and neonatal feces. We identified IgA-bound bacteria (IgA+) and characterized its diversity to elucidate possible role of IgA subclasses during neonatal bacterial colonization of the colon.
Results: We found that IgA2 in the colostrum has an active role during microbiota colonization. Colostrum IgA2 is mainly associated with Bifidobacterium, Lactobacillus, and Bacteroidetes genera. This association seems to give these bacteria an advantage during their establishment since metabolic pathways related to epithelial adhesion and carbohydrate consumption are enriched within fecal IgA2+ microbiota. Association with specific bacteria could be explained since IgA2 recognizes common antigens expressed on surfaces among bacteria genera.
Conclusions: Our data suggest a specific targeting of commensal bacteria by IgA2 revealing a specialized function of IgA microbiota association during neonatal intestinal colonization during the first days of life.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- Supplementary1.pptx
Supplementary 1. Bacterial proteases preferentially degrade IgA1. Kinetical evaluation of the stability of IgA1 (left) and IgA2 1 (right), in two different conditions of temperature and presence/absence of protease inhibitors, in contact with IgA2+ or IgA1+ 2 bacteria fractions from colostrum; in minutes (min); by ELISA. All values are represented as mean ± SD. All data are expressed 3 in milligrams of IgA subclasses per milliliter (mg/mL). Statistical analysis was performed using the Pearson correlation test. *p 4 <0.05, **p <0.01 and ***p <0.001. 5
- Supplementary2.pptx
Supplementary 2. Some of the newborn fecal microbiota have their origin in the colostrum. (A) Microbial source tracker analysis 6 showing the proportion of bacteria identified in the neonatal stool classified by source (p < 0:001, Wilcoxon signed-rank test). 7 (B) The relative abundance of most common bacterial orders found in the neonatal stool is classified by Qiime source tracker 8 analysis as "Other sources" and "Colostrum source".
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