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Danuta Izabela Kosik-Bogacka
Pomorski Uniwersytet Medyczny w Szczecinie
Corresponding Author
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Background: Acanthamoeba spp. are cosmopolitan protozoa that cause infections in the brain, as well as extracerebral infections in the cornea, lungs and skin. Little is known about the mechanisms of the immunological response to these parasites in organs which are not their main biotope. Therefore, the purpose of this study was to determine the expression of TLR2 and TLR4 in the kidneys and heart of Acanthamoeba spp. infected mice, with respect to the host’s immunological status.
Results: In the kidneys, we observed a higher expression of TLR2 in immunosuppressed mice at 24 days post Acanthamoeba spp. infection (dpi) compared to the uninfected mice. There were no statistically significant differences in TLR4 expression in the kidneys between the immunocompetent and immunosuppressed mice, both of infected and uninfected mice. In the heart, we observed a difference in TLR2 expression in immunocompetent mice at 24 dpi compared to immunocompetent mice at 8 dpi. The immunocompetent Acanthamoeba spp. infected mice had higher TLR4 expression at 8 dpi compared to the immunocompetent uninfected mice.
Conclusions: Our results indicate that TLR2 is involved in response to Acanthamoeba spp. infection in the kidneys, whereas in the heart, both studied TLRs are involved.
Keywords
Acanthamoeba spp., kidneys, heart, Toll-like receptor 2 (TLR2), Toll-like receptor 4 (TLR4)
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View the published article at Parasites & Vectors.
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Posted 30 Jun, 2020
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Editor invited
On 27 Jun, 2020
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Subject Areas
Parasitology
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A new preprint design is coming!
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See the published version of this preprint at Parasites & Vectors.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Danuta Izabela Kosik-Bogacka
Pomorski Uniwersytet Medyczny w Szczecinie
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Parasites & Vectors.
Version 1
Posted 30 Jun, 2020
No community comments so far
Editor invited
On 27 Jun, 2020
Editor assigned
On 27 Jun, 2020
First submitted
On 26 Jun, 2020
Submission checks complete
On 26 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Parasites & Vectors.
Background: Acanthamoeba spp. are cosmopolitan protozoa that cause infections in the brain, as well as extracerebral infections in the cornea, lungs and skin. Little is known about the mechanisms of the immunological response to these parasites in organs which are not their main biotope. Therefore, the purpose of this study was to determine the expression of TLR2 and TLR4 in the kidneys and heart of Acanthamoeba spp. infected mice, with respect to the host’s immunological status.
Results: In the kidneys, we observed a higher expression of TLR2 in immunosuppressed mice at 24 days post Acanthamoeba spp. infection (dpi) compared to the uninfected mice. There were no statistically significant differences in TLR4 expression in the kidneys between the immunocompetent and immunosuppressed mice, both of infected and uninfected mice. In the heart, we observed a difference in TLR2 expression in immunocompetent mice at 24 dpi compared to immunocompetent mice at 8 dpi. The immunocompetent Acanthamoeba spp. infected mice had higher TLR4 expression at 8 dpi compared to the immunocompetent uninfected mice.
Conclusions: Our results indicate that TLR2 is involved in response to Acanthamoeba spp. infection in the kidneys, whereas in the heart, both studied TLRs are involved.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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