Fluorescence Enhancement Based Quanti cation of Human Serum Albumin from Biological Sample Using Indole Based Nanosuspension: Molecular Interactions and Molecular Docking Studies

Fluorescent 3-[(E)-(2-phenylhydrazinylidene) methyl]-1H-indole (PHI) was synthesized by condensation of indole-3-carboxaldehyde and phenyl hydrazine in presence of acetic acid and ethanol and after spectral characterization used further to prepare its aqueous nano suspension by reprecipitation method using polyvinylpyrrolidone (PVP) as stabilizer. The average particle size of nano suspension measured by Dynamic Light Scattering (DLS) was found 77.5 nm while FESEM microphotograph showed spherical morphology. The blue shift in the absorption spectrum and stokes shifted fluorescence of nanosuspension of PHI compared to its monomer spectrum in dilute solution indicate formation of H-type aggregate by face to face overlapping of the molecules.The aggregation induced enhanced emission (AIEE) of PVP capped nanosuspension of PHI is increased appreciably by presence of aqueous solution of human serum albumin (HSA). A suitable mechanism of molecular binding interactions based on complex formation between PHI nanoaggregate and HSA through PVP is proposed. Fluorescence life time, zeta potential and particle size data of PHI nanoparticles (PHINPs) obtained in presence of different amounts of HSA are in support of molecular interactions leading to complex formation. The molecular docking studies showed that HSA and PVP capped PHINPs exhibit strong hydrogen bonding interaction. The fluorescence enhancement effect induced in PHI nanosuspension is used further to develop analytical method for quantitative estimation of HSA in aqueous biological sample solution.


Introduction
Pharmacodynamics plays crucial role in the drug-protein interaction [1]. Human serum albumin (HSA) is the most abundant drug carrier protein, with a well-known primary structure [2]. The distribution and bioavailability of free active concentration of administered drug is in uenced by the binding interaction of drug and HAS [3]. It is shown that the binding of drug to HSA prolonged drug half-life by lowering the free drug concentration in the blood which is essential in the clinical care. Indole derivatives have been widely screened for anti-in ammatory activity and inhibition of multiple pathways in in ammation [4]. In addition, indole derivatives are reported to possess number of potent biological activities including analgesies, antipyretics, antifungal and antimicrobial [4]. Substitution of heterocyclic moiety at the 3position of indole ring markedly in uences the anti-in ammatory activity [5][6][7]. Varieties of nitrovinylindoles derived from indole-3carboxaldehyde have shown to possess antifungal and amoebicidal activity [4]. 3-[E-(2-phenyl hydrazinylidene) methyl] 1H-indole (PHI), an indole based drug was prepared by condensation of indole-3-carboxaldehyde and phenyl hydrazine in high yield under mild laboratory condition. Synthesized PHI was characterized by 1 H NMR and 13 C NMR spectroscopy.
However poor water solubility of PHI affects bioavailability. Formulation as nano suspension is an attractive and promising alternative to resolve the problem of low bioavailability of poor water soluble drugs [8]. In addition enhanced physical and chemical stability, possibility of dose reduction and safer dose are some of the additional advantages of using nano suspensions [9]. High pressure homogenization, media milling, microemulsion, melt emulsi cationand re-precipitationare currently used methods of preparation of nanosuspensions [10][11][12][13][14]. Of this reprecipitation method is simple, economical and ecofriendly as water is dispersion medium. The other methods suffer from formation of large particles, generation of residue, toxicity of nonaqueous solvent and requirement of high amount of surfactant and stabilizer. With this in mind the suspension of PHI was prepared by reprecipitation method using polyvinylpyrrolidone (PVP) as stabilizer. Interest in PVP is because it acts as vehicle for dispensing and suspending drugand has low acute toxicity [15]. Additionally in examination of sensing performance of HSA by PHI suspension we found enhanced uorescence signals when excited using characteristic excitation wavelength of PHI. Present paper reports execution of drug-protein interaction and application to develop analytical method for the quanti cation of HSA from blood sample. The binding interactions are supported by molecular docking study.
Polyvinylpyrrolidone (PVP, K-30) was obtained from Spectrochem Pvt. Ltd. Mumbai, (India). Human serum albumin (HSA) was purchased from HiMedia laboratories Pvt. Ltd. Mumbai (India).The serum samples were collected from the Health Centre of Shivaji University, Kolhapur (India). Ultrapure water was obtained by passing distilled water through a Millipore unit (India) and was used in all experiments. Indole-3-carboxaldehyde and phenyl hydrazine (1 mmol of each) were added to a 96% ethanol containing 10 mL glacial acetic acid. The resulting mixture was heated under re ux and the reaction progress was monitored by thin layer chromatography [7]. 3-[(E)-(2-phenylhydrazinylidene) methyl] 1H-indole (PHI) was crystallized on cooling re uxed content and then ltered. The residue was washed with n-hexane, dried and recrystallized from ethanol solution. The synthesis route of PHI is shown in scheme 1. PHI is obtained as off-white powder with 95 % yield and experimental melting point 197°Cmatches with literature value 198°C. The results of 1 H NMR and 13 C NMR further con rms the formation of desired product (ESI, Fig. S1 and S2).
The content was then sonicated for 30 minutes to suspend the PHI nanoparticles (PHINPs) uniformly in aqueous dispersion medium. The aqueous suspension of PHINPs was also prepared by using different surfactants like sodium dodecyl sulphate (SDS), cetyltrimethylammonium bromide (CTAB). The particle size of nanosuspension given in Table S1 (supporting information) indicates nanosuspension prepared using PVP is of more ne particles.

Results And Discussion
Surface and Optical Properties of PVP Stabilized PHINPs The histogram of particle size distribution of PVP stabilized PHINPs presented in Fig. 1 shows narrower particle size distribution in the range from 50-100 nm and the average particle size is 77.5 nm. Fig. 2 shows FESEM images of air dried lm of aqueous suspension of PHINPs in different stabilizers and lm prepared without stabilizer. It is seen that the image of the lm prepared in PVP stabilizer reveals spherical monodispersed particles of average size 150 nm. However morphology of PHINPs prepared using other stabilizers seen irregular in SEM images. The observed larger size of the nanoparticle spheres in FESEM analysis as compared to the value obtained from DLS technique is due to agglomeration of nanoparticles which forms large size spheres during evaporation of water from aqueous suspension in the process of the preparation of thin lm on silicon wafers.
The zeta potential distribution of PVP stabilized PHINPs shown in Fig. 3 gives -24.9 mV zeta potential due to electron rich 'O' atoms of PVP capping. The hydrophilic pyrrolidone part acted as head group while the hydrophobic -[CH2-CH] n part is the tail. During the formation of nanosuspension the PHI molecules nucleate and aggregate to form dimeric species in aqueous medium. The absorption spectrum of nano suspension and that of dilute solution of PHI in acetone presented in Fig. 4 shows blue shift indicating formation of H-type of aggregates due to lateral π-stacking effect between neighboring PHI molecules in suspension [16][17]. However the uorescence spectrum (D) of nanosuspension in Fig. 5, peaking at 406 nm is seen enhanced very strongly in comparison with the weak structured uorescence of dilute solution of PHI appeared in the wavelength region of 400-500 nm (spectrum B). The broad, pronounced uorescence spectrum of nanosuspension of PHI is also shifted towards blue of the uorescence spectrum of its dilute solution. In addition Fig. 5, shows a large stoke shift of 7116 cm -1 between the excitation spectrum (C) and uorescence spectrum (D) of nanosuspension of PHI. On the contrary the stoke shift value of 3932 cm -1 estimated from excitation spectrum (A) and uorescence spectrum (B) of dilute solution of PHI in acetone is small. These observations suggest that the uorescence of nanosuspension is aggregation induced enhanced emission (AIEE). The results of pH dependence of uorescence intensity presented in Fig. 6 show maxima at pH 7.5.

Recognition Test of Nanosuspension of PHI for HSA
The uorescence spectra produced using 315 nm excitation wavelength of nanosuspension of PHI in presence of various biologically important molecules such as HSA, bovine albumin serum (BSA), Dpenicillamine, guanine, sucrose, glucose, cyanocobalmine, hemoglobin, vit.B-1, glutathione each of 1.8 µM concentration are given in Fig. 7. The careful observation of the spectra reveals that the uorescence of nano suspension of PHI is enhanced signi cantly by HSA as compared to enhancement induced by BSA, while other biomolecules decreases the uorescence of nanosuspension. In addition to this the bar diagram in Fig. 8 indicating effect of uorescence change estimated as, ('F 0 '-is the intrinsic uorescence of nanosuspension and 'F' is the uorescence in presence of biomolecules) supports to the observation of recognition of HSA by suspended nanoparticles of PHI. The blue bar in Fig. 8 shows enhancement effect by HSA and BSA. The enhancement produced by HSA is signi cantly large in comparison with BSA. The orange bar seen in the same gure reveals that the uorescence enhancement induced by HSA is not affected even in presence of coexisting substances. In addition to these the comparison of the uorescence spectrum of nanosuspension containing HSA with the pure spectrum without HSA shown a blue shift of about 2035cm -1 while BSA does not produced any spectral shift. This observation led to consider binding between PHI nanoaggregate and HSA molecule only.

Studies on Binding Interactions between HSA and PHI Nanoparticles
The gradually increasing amounts of HSA solution in the concentration range 0.1-1.8 µM were added to de nite amount of nanosuspension of PHI buffered at pH 7.5 taken in the different test tubes (ESI , Table  S2). Fluorescence spectra of the nanosuspension monitored at excitation wavelength of 315 nm in presence of HSA and without HSA are shown in Fig. 9. From the spectra it is seen that the uorescence intensity of PHI nanosuspension not only increases signi cantly but also the wavelength of maximum emission shifts from 406 nm to 375 nm as concentrations of HSA increases.
The uorescence enhancement and observed wavelength shift effect are discussed on the basis of complex formation between PVP capped PHI nanosuspension through the electron rich 'O' atoms of PVP with HSA molecule. It is thought that PVP may acts as receptor between PHINPs and HSA. This proposed binding interaction mechanism further supported by the double logarithmic plot [18].The binding constant 'K' and binding sites 'n' are estimated from the double logarithmic plot Log 10 (F 0 -F)/F Vs Log [HSA] as shown in Fig. 10. This plot is based on equation 1 given below.
where F 0 and F are the uorescence intensities of PHINPs in absence and presence of HSA concentration.
The value of K obtained from the plot is 7.030 × 10 5 Lit. mol -1 indicates good stability of complex while the value of binding site (n) nearly equal to 1 indicates availability of one possible binding site on the surface of suspended nanoparticles.
Fluorescence lifetime determinations of nanosuspension of PHI in presence of HSA and without HSA are found in harmony with the proposed mechanism of molecular interaction leading to formation of complex. Fig. 11 shows the decay pro le of PHINPs in absence and in presence of HSA solution. The life time 7.29 ns (Fig. 11[A]) of suspension without HSA found to increase in order of 8.02 ns, 8.52 ns and 10.61 ns (Fig. 11[B-D]) when concentration of HSA solution was 0.3 μM, 0.4 μM and 1.0 μM respectively. The molecular interactions and complexation lead to prolonged uorescence lifetime [19].Thus increase in lifetime of suspension with addition of HSA suggests stabilization of PVP-PHINP-HSA complex. The schematic representation of uorescence enhancement of PHINPs upon interaction with HSA is graphically presented in scheme 2. The average particle size and zeta potential measurements further con rm complex formation between PHI nanoparticles and HSA. The zeta potential of as prepared nanosuspension -24.9 mV seen in Fig. 12 decreases to -16.7 mV and to -10.6 mV when concentration of HSA increased from 0.5 μM and to 1.0 μM, while at the same time the particle size seen increasing from 77.5 nm to 250 nm and to 481.1 nm respectively. The increase in particle size and decrease in zeta potential led to consider adsorption of HSA over the negatively charged surface of suspended particles [20]. Thus the observed uorescence enhancement is attributed to the possible interaction with negatively charged oxygen atom of PVP with HSA as presented in scheme 2.

Exploration of mechanism of binding between PVP capped PHINPs and HSA by absorption spectroscopy
The absorption spectra of the PHINPs in presence of different amounts of HSA showed in Fig. S3 reveals the red shift in wavelength of maximum absorbance as indicating complex formation between PVP capped PHINPs and HSA. The PVP acts as receptor between them for enhancing the rate of charge transfer in complex formation.

Molecular Docking Studies
To provide further, the deeper insight into the interaction of HSA with PHINPs, a molecular docking technique is used. The experiment of docking studies carried out between human serum albumin (2bx8.pdb) and PVP-N-PHI is shown in Fig. 13. The three-dimensional structure of human serum albumin (2bx8.pdb) was extracted from RCSB PDB. Further the three dimensional structure of PVP-N-PHI was built by SPARTAN ver 6.0.1 Softwareand minimized by using HartreeFock (HF) method in the SPARTAN ver 6.0.1 Software [21][22]. After completion of minimization the PVP-PHINPs structure with less energy were used for the molecular docking with human serum albumin (2bx8.pdb). Finally, the docked complex of PVP-N-PHI with human serum albumin (2bx8.pdb) was analyzed by CHIMERA.The best conformation with least binding energy of 5.02KJ/mol was visualized as presented in Fig. 13.
The best possible solution obtained by the molecular docking study suggests that PVP capped PHI nanoparticle is able to interact with HSA by means of hydrogen bonding. The amino acid residues of human serum albumin (2bx8.pdb) such as TYR-150, GLN-196, HIS -242, LEU-238, ARG-257 are having strong hydrogen bonding interactions with the PVP-PHINPs. These interactions are predicted to occur at distances required for effective H-bonding ranging from 1.837Å to 2.774 Å (Table 1) Where,'s' is the standard deviation of 'y' intercept of the regression line, 'K' is the slope of the calibration graph. An analytical method is developed for the quanti cation of HSA in the serum samples collected from the Health Centre of Shivaji University, Kolhapur. The blood samples were allowed to clot at room temperature for 15-30 minutes and then the clot was removed by centrifuging at 2000 rpm for 10 minutes in refrigerated centrifuge. The resulting supernatant liquid is serum. Serum consists of antibodies, antigens, electrolytes, hormones and proteins [24].An appropriate amount of the serum samples were spiked with HSA to prepare two synthetic samples of concentration 0.3 µM and 0.7 µM, and diluted by distilled water so that uorescence intensity of samplesolutions is in the working range of calibration curve. The results of analysis shown in Table 2 indicate that the amount of HSA found by proposed method is in close agreement with amount added with good percent recovery.

Supplementary Information
Supplementary Files were not provided with this version of the manuscript. Figure 1 Particle size distribution histogram of PHINPs from DLS analysis.   Absorption spectra of PHI in acetone (spectrum A) and PHINPs in aqueous suspension (spectrum B).  Effect of pH on the uorescence intensity of aqueous nanosuspension.