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Macromolecule Biomaterials Promote the Osteogenic Differentiation Capacity of Equine Adipose -Derived Mesenchymal Stem Cells
Mohamed I. Elashry
Justus Liebig Universitat Giessen
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6312-2182
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Stem Cell Research & Therapy.
Background: Combination of mesenchymal stem cells (MSCs) and biomaterials is a rapidly growing approach in regenerative medicine particularly for chronic degenerative disorders including osteoarthritis and osteoporosis. In the present study, the effect of biomaterial bone substitutes on equine adipose derived MSCs morphology, viability, adherence, migration and osteogenic differentiation were investigated.
Methods: MSCs were cultivated in conjunction with collagen CultiSpher-S Microcarrier (MC), nanocomposite xerogels B30 and B30Str biomaterials in osteogenic differentiate medium either under static or mechanical fluid shear stress (FSS) culture conditions. The data were generated by histological means, life cell imaging, cell viability, adherence and migration assays. Osteogenic differentiation was detected by semi-quantification of alkaline phosphatase (ALP) activity, matrix mineralization using Alizarin Red S (ARS) staining and quantification of the osteogenic markers; runt related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) expression using RT-qPCR. All data were statistically analyzed using ANOVA.
Results: The data revealed that combined mechanical stress with MC but not B30 enhanced MSCs viability and promoted their migration. Combined osteogenic medium with MC, B30 and B30Str increased ALP activity compared to cultivation in basal medium. Osteogenic induction with MC, B30 and B30Str resulted in diffused matrix mineralization by means of ARS. FSS increased the viability in the presence of the osteogenic medium with MC but not B30 or B30Str. FSS enhanced osteogenic differentiation in the presence of B30Str. Upregulation of Runx2 and ALP expression was detected with osteogenic differentiation together with B30 and B30Str regardless of static or FSS culture.
Conclusions: Taken together, the data revealed that FSS in conjunction with biomaterials promoted osteogenic differentiation of MSCs. This combination may be considered as a marked improvement for clinical applications to cure bone defects.
Keywords
Stem cells, osteogenic differentiation, biomaterials
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On 12 Feb, 2021
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On 03 Jan, 2021
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Editorial decision: Minor Revision
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Received 06 Dec, 2020
Reviewer #3 agreed
On 30 Nov, 2020
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Received 29 Nov, 2020
Reviewer #2 agreed
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Reviewer #1 agreed
On 25 Nov, 2020
Editor assigned
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Reviewers invited
Invitations sent on 23 Nov, 2020
Submission checks complete
On 23 Nov, 2020
Editor invited
On 23 Nov, 2020
Version 1
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Editorial decision: Major Revision
On 21 Aug, 2020
Review #5 received
Received 17 Aug, 2020
Reviewer #6 agreed
On 13 Aug, 2020
Review #4 received
Received 13 Aug, 2020
Review #3 received
Received 13 Aug, 2020
Reviewer #5 agreed
On 08 Aug, 2020
Reviewer #4 agreed
On 07 Aug, 2020
Review #2 received
Received 16 Jul, 2020
Reviewer #3 agreed
On 16 Jul, 2020
Review #1 received
Received 06 Jul, 2020
Reviewer #1 agreed
On 02 Jul, 2020
Reviewer #2 agreed
On 02 Jul, 2020
Reviewers invited
Invitations sent on 01 Jul, 2020
Editor assigned
On 30 Jun, 2020
Submission checks complete
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On 29 Jun, 2020
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This preprint is under consideration at Stem Cell Research & Therapy. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Macromolecule Biomaterials Promote the Osteogenic Differentiation Capacity of Equine Adipose -Derived Mesenchymal Stem Cells
Mohamed I. Elashry
Justus Liebig Universitat Giessen
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6312-2182
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Published
On 12 Feb, 2021
No community comments so far
Editorial decision: Accept
On 03 Jan, 2021
Review #2 received
Received 30 Dec, 2020
Review #1 received
Received 24 Dec, 2020
Reviewer #3 agreed
On 23 Dec, 2020
Review #3 received
Received 23 Dec, 2020
Reviewer #2 agreed
On 22 Dec, 2020
Reviewers invited
Invitations sent on 22 Dec, 2020
Reviewer #1 agreed
On 22 Dec, 2020
Editor assigned
On 20 Dec, 2020
Submission checks complete
On 20 Dec, 2020
Editor invited
On 20 Dec, 2020
No comments provided
Editorial decision: Minor Revision
On 13 Dec, 2020
Review #3 received
Received 06 Dec, 2020
Reviewer #3 agreed
On 30 Nov, 2020
Review #2 received
Received 29 Nov, 2020
Reviewer #2 agreed
On 28 Nov, 2020
Review #1 received
Received 26 Nov, 2020
Reviewer #1 agreed
On 25 Nov, 2020
Editor assigned
On 23 Nov, 2020
Reviewers invited
Invitations sent on 23 Nov, 2020
Submission checks complete
On 23 Nov, 2020
Editor invited
On 23 Nov, 2020
Version 1
Posted 01 Jul, 2020
No comments provided
Editorial decision: Major Revision
On 21 Aug, 2020
Review #5 received
Received 17 Aug, 2020
Reviewer #6 agreed
On 13 Aug, 2020
Review #4 received
Received 13 Aug, 2020
Review #3 received
Received 13 Aug, 2020
Reviewer #5 agreed
On 08 Aug, 2020
Reviewer #4 agreed
On 07 Aug, 2020
Review #2 received
Received 16 Jul, 2020
Reviewer #3 agreed
On 16 Jul, 2020
Review #1 received
Received 06 Jul, 2020
Reviewer #1 agreed
On 02 Jul, 2020
Reviewer #2 agreed
On 02 Jul, 2020
Reviewers invited
Invitations sent on 01 Jul, 2020
Editor assigned
On 30 Jun, 2020
Submission checks complete
On 29 Jun, 2020
Editor invited
On 29 Jun, 2020
First submitted
On 25 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Stem Cell & Developmental Cell Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Stem Cell Research & Therapy.
Background: Combination of mesenchymal stem cells (MSCs) and biomaterials is a rapidly growing approach in regenerative medicine particularly for chronic degenerative disorders including osteoarthritis and osteoporosis. In the present study, the effect of biomaterial bone substitutes on equine adipose derived MSCs morphology, viability, adherence, migration and osteogenic differentiation were investigated.
Methods: MSCs were cultivated in conjunction with collagen CultiSpher-S Microcarrier (MC), nanocomposite xerogels B30 and B30Str biomaterials in osteogenic differentiate medium either under static or mechanical fluid shear stress (FSS) culture conditions. The data were generated by histological means, life cell imaging, cell viability, adherence and migration assays. Osteogenic differentiation was detected by semi-quantification of alkaline phosphatase (ALP) activity, matrix mineralization using Alizarin Red S (ARS) staining and quantification of the osteogenic markers; runt related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) expression using RT-qPCR. All data were statistically analyzed using ANOVA.
Results: The data revealed that combined mechanical stress with MC but not B30 enhanced MSCs viability and promoted their migration. Combined osteogenic medium with MC, B30 and B30Str increased ALP activity compared to cultivation in basal medium. Osteogenic induction with MC, B30 and B30Str resulted in diffused matrix mineralization by means of ARS. FSS increased the viability in the presence of the osteogenic medium with MC but not B30 or B30Str. FSS enhanced osteogenic differentiation in the presence of B30Str. Upregulation of Runx2 and ALP expression was detected with osteogenic differentiation together with B30 and B30Str regardless of static or FSS culture.
Conclusions: Taken together, the data revealed that FSS in conjunction with biomaterials promoted osteogenic differentiation of MSCs. This combination may be considered as a marked improvement for clinical applications to cure bone defects.
Figure 1
Figure 2
Figure 3
Figure 4