See the published version of this preprint at Stem Cell Research & Therapy.
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Mohamed I. Elashry
Justus Liebig Universitat Giessen
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6312-2182
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Background: Combination of mesenchymal stem cells (MSCs) and biomaterials is a rapidly growing approach in regenerative medicine particularly for chronic degenerative disorders including osteoarthritis and osteoporosis. The present study examined the effect of biomaterial scaffolds on equine adipose derived MSCs morphology, viability, adherence, migration and osteogenic differentiation.
Methods: MSCs were cultivated in conjunction with collagen CultiSpher-S Microcarrier (MC), nanocomposite xerogels B30 and combined B30 with strontium (B30Str) biomaterials in osteogenic differentiation medium either under static or mechanical fluid shear stress (FSS) culture conditions. The data were generated by histological means, live cell imaging, cell viability, adherence and migration assays, semi-quantification of alkaline phosphatase (ALP) activity and quantification of the osteogenic markers runt related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) expression.
Results: The data revealed that combined mechanical FSS with MC but not B30 enhanced MSCs viability and promoted their migration. Combined osteogenic medium with MC, B30 and B30Str increased ALP activity compared to cultivation in basal medium. Osteogenic induction with MC, B30 and B30Str resulted in diffused matrix mineralization. The combined osteogenic induction with biomaterials under mechanical FSS increased Runx2 protein expression either in comparison to those cells cultivated in BM or those cells induced under static culture. Runx2 and ALP expression was upregulated following combined osteogenic differentiation together with B30 and B30Str regardless of static or FSS culture.
Conclusions: Taken together, the data revealed that FSS in conjunction with biomaterials promoted osteogenic differentiation of MSCs. This combination may be considered as a marked improvement for clinical applications to cure bone defects.
Keywords
Stem cells, osteogenic differentiation, biomaterials, fluid shear stress
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CURRENT STATUS: Published
View the published article at Stem Cell Research & Therapy.
Version 3
Posted 05 Jan, 2021
No community comments so far
Editorial decision: Accept
On 03 Jan, 2021
Review #2 received
Received 30 Dec, 2020
Review #1 received
Received 24 Dec, 2020
Reviewer #3 agreed
On 23 Dec, 2020
Review #3 received
Received 23 Dec, 2020
Reviewer #2 agreed
On 22 Dec, 2020
Reviewers invited
Invitations sent on 22 Dec, 2020
Reviewer #1 agreed
On 22 Dec, 2020
Editor assigned
On 20 Dec, 2020
Submission checks complete
On 20 Dec, 2020
Editor invited
On 20 Dec, 2020
No comments provided
Editorial decision: Minor Revision
On 13 Dec, 2020
Review #3 received
Received 06 Dec, 2020
Reviewer #3 agreed
On 30 Nov, 2020
Review #2 received
Received 29 Nov, 2020
Reviewer #2 agreed
On 28 Nov, 2020
Review #1 received
Received 26 Nov, 2020
Reviewer #1 agreed
On 25 Nov, 2020
Editor assigned
On 23 Nov, 2020
Reviewers invited
Invitations sent on 23 Nov, 2020
Submission checks complete
On 23 Nov, 2020
Editor invited
On 23 Nov, 2020
No comments provided
Editorial decision: Major Revision
On 21 Aug, 2020
Review #5 received
Received 17 Aug, 2020
Reviewer #6 agreed
On 13 Aug, 2020
Review #4 received
Received 13 Aug, 2020
Review #3 received
Received 13 Aug, 2020
Reviewer #5 agreed
On 08 Aug, 2020
Reviewer #4 agreed
On 07 Aug, 2020
Review #2 received
Received 16 Jul, 2020
Reviewer #3 agreed
On 16 Jul, 2020
Review #1 received
Received 06 Jul, 2020
Reviewer #1 agreed
On 02 Jul, 2020
Reviewer #2 agreed
On 02 Jul, 2020
Reviewers invited
Invitations sent on 01 Jul, 2020
Editor assigned
On 30 Jun, 2020
Submission checks complete
On 29 Jun, 2020
Editor invited
On 29 Jun, 2020
First submitted
On 25 Jun, 2020
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Stem Cell & Developmental Cell Biology
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See the published version of this preprint at Stem Cell Research & Therapy.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Mohamed I. Elashry
Justus Liebig Universitat Giessen
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6312-2182
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Stem Cell Research & Therapy.
Version 3
Posted 05 Jan, 2021
No community comments so far
Editorial decision: Accept
On 03 Jan, 2021
Review #2 received
Received 30 Dec, 2020
Review #1 received
Received 24 Dec, 2020
Reviewer #3 agreed
On 23 Dec, 2020
Review #3 received
Received 23 Dec, 2020
Reviewer #2 agreed
On 22 Dec, 2020
Reviewers invited
Invitations sent on 22 Dec, 2020
Reviewer #1 agreed
On 22 Dec, 2020
Editor assigned
On 20 Dec, 2020
Submission checks complete
On 20 Dec, 2020
Editor invited
On 20 Dec, 2020
No comments provided
Editorial decision: Minor Revision
On 13 Dec, 2020
Review #3 received
Received 06 Dec, 2020
Reviewer #3 agreed
On 30 Nov, 2020
Review #2 received
Received 29 Nov, 2020
Reviewer #2 agreed
On 28 Nov, 2020
Review #1 received
Received 26 Nov, 2020
Reviewer #1 agreed
On 25 Nov, 2020
Editor assigned
On 23 Nov, 2020
Reviewers invited
Invitations sent on 23 Nov, 2020
Submission checks complete
On 23 Nov, 2020
Editor invited
On 23 Nov, 2020
No comments provided
Editorial decision: Major Revision
On 21 Aug, 2020
Review #5 received
Received 17 Aug, 2020
Reviewer #6 agreed
On 13 Aug, 2020
Review #4 received
Received 13 Aug, 2020
Review #3 received
Received 13 Aug, 2020
Reviewer #5 agreed
On 08 Aug, 2020
Reviewer #4 agreed
On 07 Aug, 2020
Review #2 received
Received 16 Jul, 2020
Reviewer #3 agreed
On 16 Jul, 2020
Review #1 received
Received 06 Jul, 2020
Reviewer #1 agreed
On 02 Jul, 2020
Reviewer #2 agreed
On 02 Jul, 2020
Reviewers invited
Invitations sent on 01 Jul, 2020
Editor assigned
On 30 Jun, 2020
Submission checks complete
On 29 Jun, 2020
Editor invited
On 29 Jun, 2020
First submitted
On 25 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Stem Cell & Developmental Cell Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Stem Cell Research & Therapy.
Background: Combination of mesenchymal stem cells (MSCs) and biomaterials is a rapidly growing approach in regenerative medicine particularly for chronic degenerative disorders including osteoarthritis and osteoporosis. The present study examined the effect of biomaterial scaffolds on equine adipose derived MSCs morphology, viability, adherence, migration and osteogenic differentiation.
Methods: MSCs were cultivated in conjunction with collagen CultiSpher-S Microcarrier (MC), nanocomposite xerogels B30 and combined B30 with strontium (B30Str) biomaterials in osteogenic differentiation medium either under static or mechanical fluid shear stress (FSS) culture conditions. The data were generated by histological means, live cell imaging, cell viability, adherence and migration assays, semi-quantification of alkaline phosphatase (ALP) activity and quantification of the osteogenic markers runt related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) expression.
Results: The data revealed that combined mechanical FSS with MC but not B30 enhanced MSCs viability and promoted their migration. Combined osteogenic medium with MC, B30 and B30Str increased ALP activity compared to cultivation in basal medium. Osteogenic induction with MC, B30 and B30Str resulted in diffused matrix mineralization. The combined osteogenic induction with biomaterials under mechanical FSS increased Runx2 protein expression either in comparison to those cells cultivated in BM or those cells induced under static culture. Runx2 and ALP expression was upregulated following combined osteogenic differentiation together with B30 and B30Str regardless of static or FSS culture.
Conclusions: Taken together, the data revealed that FSS in conjunction with biomaterials promoted osteogenic differentiation of MSCs. This combination may be considered as a marked improvement for clinical applications to cure bone defects.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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