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Research article
Guiping Zhao
Foshan University, Chinese Academy of Agricultural Sciences
Corresponding Author
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Background: The body distribution with high intramuscular fat and low abdominal fat is ideal goal for broiler breeding. Preadipocytes with different origins have differences in metabolism and gene expression. This transcriptome analysis of intramuscular preadipocytes (DIMPs) and adipose tissue-derived preadipocytes (DAFPs) is aim to explore the characteristics in lipid deposition of different chicken preadipocytes by dedifferentiation in vitro.
Results: Compared to DAFPs, the total lipid content was decreased (P <0.05) in DIMFPs after two days with 100% confluence. Moreover, 72 DEGs related to lipid metabolism were screened, which are involved in the adipocyte differentiation, fatty acid transport and fatty acid synthesis, lipid stabilization, and lipolysis. Among the 72 DEGs, 19 DEGs were enriched in the PPAR signaling pathway, indicating a main contribution to the regulation of the difference of lipid deposition between DAFPs and DIMFPs. Among these 19 genes, the representative APOA1, ADIPOQ, FABP3, FABP4, FABP7, HMGCS2, LPL and RXRG genes were down-regulated, but ACSL1, FABP5, PCK2, PDPK1, PPARG, SCD, SCD5, SLC27A6 genes were up-regulated (P < 0.05 or P < 0.01) in the DIMFPs. In addition, the well-known pathways affecting lipid metabolism (MAPK-, TGF beta-, Calcium-, PPAR signaling pathway) and the pathways related to cell communication were enriched, which may also contribute to the regulation of lipid deposition. Finally, the regulatory network for the difference of lipid deposition between chicken DAFPs and DIMFPs were proposed based on the above information.
Conclusions: Our data suggested the difference of lipid deposition between DIMPs and DAFPs of chicken in vitro, and proposed the molecular regulatory network for the difference of lipid deposition between chicken DAFPs and DIMFPs. The lipid content was significantly increased in DAFPs by the direct mediation of PPAR signaling pathways. These findings provide new insights into the regulation of tissue-specific fat deposition and optimizing body fat distribution in broilers.
Keywords
Dedifferentiated preadipocytes, Different tissue derivation, lipid deposition, DEGs, Chicken
Figure 1
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Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1TableS1.xls
Additional file 1: Table S1: The expression genes (DIMFPs vs DAFPs). (XLS 13006 kb)
- Additionalfile2TableS2.xlsx
Additional file 2: Table S2: The screened known DEGs (DIMFPs vs DAFPs). (XLS 145 kb)
- Additionalfile3TableS3.xls
Additional file 3: Table S3: The enriched GO terms based on DEGs (DIMFPs vs DAFPs). (XLS 396 kb)
- Additionalfile4TableS4.xls
Additional file 4: Table S4: The enriched pathways based on DEGs (DIMFPs vs DAFPs). (XLS 40 kb)
- Additionalfile5TableS5.xls
Additional file 5: Table S5: The screened DEGs related to lipid metabolism (DIMFPs vs DAFPs). (XLS 44 kb)
- Additionalfile6TableS6.xls
Additional file 6: Table S6: The information on the specific primer sued for qRT-PCR. (XLS 16 kb)
- Additionalfile7FigureS1.png
Additional file 7: Figure S1: DEGs involved in the PPAR signaling pathway (map 03320). The red box plot shows downregulated genes, and the green box plot shows upregulated genes in the pathway (DIMFPs vs DAFPs). The figure has obtained the KEGG copyright permission. (PNG 33.5 kb)
- Additionalfile8FigureS2.png
Additional file 8: Figure S2: DEGs involved in the MAPK signaling pathway (map 04010). The red box plot shows downregulated genes, and the green box plot shows upregulated genes in the pathway (DIMFPs vs DAFPs). The figure has obtained the KEGG copyright permission. (PNG 44.4 kb)
- Additionalfile9FigureS3.png
Additional file 9: Figure S3: DEGs involved in the Calcium signaling pathway (map 04020). The red box plot shows downregulated genes, and the green box plot shows upregulated genes in the pathway (DIMFPs vs DAFPs). The figure has obtained the KEGG copyright permission. (PNG 29.5 kb)
- Additionalfile10FigureS4.png
Additional file 10: Figure S4: DEGs involved in the TGF beta signaling pathway (map 04350). The red box plot shows downregulated genes, and the green box plot shows upregulated genes in the pathway (DIMFPs vs DAFPs). The figure has obtained the KEGG copyright permission. (PNG 29.3 kb)
- Additionalfile11KEGGPERMISSION.pdf
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View the published article at BMC Genomics.
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Editorial decision: Minor revision
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Reviewer #2 agreed
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Editorial decision: Major revision
On 26 Sep, 2020
Reviewer #5 agreed
On 19 Sep, 2020
Review #2 received
Received 18 Sep, 2020
Reviewer #3 agreed
On 18 Sep, 2020
Reviewer #4 agreed
On 18 Sep, 2020
Review #3 received
Received 18 Sep, 2020
Review #1 received
Received 18 Sep, 2020
Reviewer #2 agreed
On 15 Sep, 2020
Reviewer #1 agreed
On 15 Sep, 2020
Reviewers invited
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Editor assigned
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A new preprint design is coming!
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See the published version of this preprint at BMC Genomics.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Guiping Zhao
Foshan University, Chinese Academy of Agricultural Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Genomics.
Version 2
Posted 14 Jan, 2021
No community comments so far
Editorial decision: Minor revision
On 02 Feb, 2021
Review #2 received
Received 21 Jan, 2021
Reviewer #2 agreed
On 15 Jan, 2021
Review #1 received
Received 10 Jan, 2021
Reviewer #1 agreed
On 09 Jan, 2021
Reviewers invited
Invitations sent on 09 Jan, 2021
Editor assigned
On 05 Jan, 2021
Submission checks complete
On 05 Jan, 2021
Editor invited
On 05 Jan, 2021
No comments provided
Editorial decision: Major revision
On 26 Sep, 2020
Reviewer #5 agreed
On 19 Sep, 2020
Review #2 received
Received 18 Sep, 2020
Reviewer #3 agreed
On 18 Sep, 2020
Reviewer #4 agreed
On 18 Sep, 2020
Review #3 received
Received 18 Sep, 2020
Review #1 received
Received 18 Sep, 2020
Reviewer #2 agreed
On 15 Sep, 2020
Reviewer #1 agreed
On 15 Sep, 2020
Reviewers invited
Invitations sent on 13 Sep, 2020
Editor assigned
On 23 Jul, 2020
Submission checks complete
On 22 Jul, 2020
Editor invited
On 22 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Genomics.
Background: The body distribution with high intramuscular fat and low abdominal fat is ideal goal for broiler breeding. Preadipocytes with different origins have differences in metabolism and gene expression. This transcriptome analysis of intramuscular preadipocytes (DIMPs) and adipose tissue-derived preadipocytes (DAFPs) is aim to explore the characteristics in lipid deposition of different chicken preadipocytes by dedifferentiation in vitro.
Results: Compared to DAFPs, the total lipid content was decreased (P <0.05) in DIMFPs after two days with 100% confluence. Moreover, 72 DEGs related to lipid metabolism were screened, which are involved in the adipocyte differentiation, fatty acid transport and fatty acid synthesis, lipid stabilization, and lipolysis. Among the 72 DEGs, 19 DEGs were enriched in the PPAR signaling pathway, indicating a main contribution to the regulation of the difference of lipid deposition between DAFPs and DIMFPs. Among these 19 genes, the representative APOA1, ADIPOQ, FABP3, FABP4, FABP7, HMGCS2, LPL and RXRG genes were down-regulated, but ACSL1, FABP5, PCK2, PDPK1, PPARG, SCD, SCD5, SLC27A6 genes were up-regulated (P < 0.05 or P < 0.01) in the DIMFPs. In addition, the well-known pathways affecting lipid metabolism (MAPK-, TGF beta-, Calcium-, PPAR signaling pathway) and the pathways related to cell communication were enriched, which may also contribute to the regulation of lipid deposition. Finally, the regulatory network for the difference of lipid deposition between chicken DAFPs and DIMFPs were proposed based on the above information.
Conclusions: Our data suggested the difference of lipid deposition between DIMPs and DAFPs of chicken in vitro, and proposed the molecular regulatory network for the difference of lipid deposition between chicken DAFPs and DIMFPs. The lipid content was significantly increased in DAFPs by the direct mediation of PPAR signaling pathways. These findings provide new insights into the regulation of tissue-specific fat deposition and optimizing body fat distribution in broilers.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1TableS1.xls
Additional file 1: Table S1: The expression genes (DIMFPs vs DAFPs). (XLS 13006 kb)
- Additionalfile2TableS2.xlsx
Additional file 2: Table S2: The screened known DEGs (DIMFPs vs DAFPs). (XLS 145 kb)
- Additionalfile3TableS3.xls
Additional file 3: Table S3: The enriched GO terms based on DEGs (DIMFPs vs DAFPs). (XLS 396 kb)
- Additionalfile4TableS4.xls
Additional file 4: Table S4: The enriched pathways based on DEGs (DIMFPs vs DAFPs). (XLS 40 kb)
- Additionalfile5TableS5.xls
Additional file 5: Table S5: The screened DEGs related to lipid metabolism (DIMFPs vs DAFPs). (XLS 44 kb)
- Additionalfile6TableS6.xls
Additional file 6: Table S6: The information on the specific primer sued for qRT-PCR. (XLS 16 kb)
- Additionalfile7FigureS1.png
Additional file 7: Figure S1: DEGs involved in the PPAR signaling pathway (map 03320). The red box plot shows downregulated genes, and the green box plot shows upregulated genes in the pathway (DIMFPs vs DAFPs). The figure has obtained the KEGG copyright permission. (PNG 33.5 kb)
- Additionalfile8FigureS2.png
Additional file 8: Figure S2: DEGs involved in the MAPK signaling pathway (map 04010). The red box plot shows downregulated genes, and the green box plot shows upregulated genes in the pathway (DIMFPs vs DAFPs). The figure has obtained the KEGG copyright permission. (PNG 44.4 kb)
- Additionalfile9FigureS3.png
Additional file 9: Figure S3: DEGs involved in the Calcium signaling pathway (map 04020). The red box plot shows downregulated genes, and the green box plot shows upregulated genes in the pathway (DIMFPs vs DAFPs). The figure has obtained the KEGG copyright permission. (PNG 29.5 kb)
- Additionalfile10FigureS4.png
Additional file 10: Figure S4: DEGs involved in the TGF beta signaling pathway (map 04350). The red box plot shows downregulated genes, and the green box plot shows upregulated genes in the pathway (DIMFPs vs DAFPs). The figure has obtained the KEGG copyright permission. (PNG 29.3 kb)
- Additionalfile11KEGGPERMISSION.pdf
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