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Research Article
Guojun Zhang
Zhengzhou University First Affiliated Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1756-0082
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Non-small cell lung cancer (NSCLC) patients are basically at an advanced stage once diagnosed. In this study, our aimed to identify a new biomarker for early diagnosis of NSCLC.
Methods and materials: CircRNA array analysis was designed to study the expression patterns of circRNAs in three pairs of NSCLC tissues. The expression of hsa_circ_0054284 were detected in 30 paired NSCLC tissues and adjacent normal tissues by qRT-PCR assay. A549 and H520 cells were transfected with overexpression vector of hsa_circ_0054284 and negative control. CCK-8, transwell invasion and Cell apoptosis assay were using to explore the internal relationship among the hsa_circ_0054284, miR-18a-3p and TIMP2. TIMP2 expression level was detected by qRT-PCR assay and western blotting analysis.
Results: Hsa-circ-0054284 overexpression suppressed A549/H520 cell proliferation and invasion and promoted apoptosis via downregulation of miR-18a-3p and targeting TIMP2. This might be one of the possible mechanisms, which hsa-circ-0054284 plays in NSCLC.
Conclusion: The current study provides novel insights into the circRNA-related ceRNA network in NSCLC and the hsa-circ-0054284 biomarkers may be Early diagnosis in NSCLC patients.
Keywords
hsa_circ_0054284, miR-18a-3p, non-small-cell lung cancer, TIMP2
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CURRENT STATUS: Under Revision
Version 1
Posted 16 Apr, 2021
No community comments so far
Editorial decision: Major revision
On 30 Apr, 2021
Review #3 received
Received 29 Apr, 2021
Reviewer #3 agreed
On 27 Apr, 2021
Review #1 received
Received 26 Apr, 2021
Reviewer #2 agreed
On 13 Apr, 2021
Review #2 received
Received 13 Apr, 2021
Reviews received
Received 13 Apr, 2021
Reviewers invited
Invitations sent on 12 Apr, 2021
Reviewer #1 agreed
On 12 Apr, 2021
Editor assigned
On 09 Apr, 2021
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On 09 Apr, 2021
Editor invited
On 09 Apr, 2021
First submitted
On 31 Mar, 2021
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This preprint is under consideration at Cancer Cell International. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Guojun Zhang
Zhengzhou University First Affiliated Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1756-0082
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 16 Apr, 2021
No community comments so far
Editorial decision: Major revision
On 30 Apr, 2021
Review #3 received
Received 29 Apr, 2021
Reviewer #3 agreed
On 27 Apr, 2021
Review #1 received
Received 26 Apr, 2021
Reviewer #2 agreed
On 13 Apr, 2021
Review #2 received
Received 13 Apr, 2021
Reviews received
Received 13 Apr, 2021
Reviewers invited
Invitations sent on 12 Apr, 2021
Reviewer #1 agreed
On 12 Apr, 2021
Editor assigned
On 09 Apr, 2021
Submission checks complete
On 09 Apr, 2021
Editor invited
On 09 Apr, 2021
First submitted
On 31 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Non-small cell lung cancer (NSCLC) patients are basically at an advanced stage once diagnosed. In this study, our aimed to identify a new biomarker for early diagnosis of NSCLC.
Methods and materials: CircRNA array analysis was designed to study the expression patterns of circRNAs in three pairs of NSCLC tissues. The expression of hsa_circ_0054284 were detected in 30 paired NSCLC tissues and adjacent normal tissues by qRT-PCR assay. A549 and H520 cells were transfected with overexpression vector of hsa_circ_0054284 and negative control. CCK-8, transwell invasion and Cell apoptosis assay were using to explore the internal relationship among the hsa_circ_0054284, miR-18a-3p and TIMP2. TIMP2 expression level was detected by qRT-PCR assay and western blotting analysis.
Results: Hsa-circ-0054284 overexpression suppressed A549/H520 cell proliferation and invasion and promoted apoptosis via downregulation of miR-18a-3p and targeting TIMP2. This might be one of the possible mechanisms, which hsa-circ-0054284 plays in NSCLC.
Conclusion: The current study provides novel insights into the circRNA-related ceRNA network in NSCLC and the hsa-circ-0054284 biomarkers may be Early diagnosis in NSCLC patients.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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