Mining waste creates challenging soil conditions that hinder tree establishment for boreal forest restoration. This study investigated the effects of adding topsoil or ramial chipped wood (RCW) on the physicochemical properties of waste rock and the growth and survival of planted native tree species. An randomized-block setup with four treatments and repetitions was established on a gold mine site in western Quebec, Canada in 2018, and planted with Pinus banksiana and Abies balsamea. Results demonstrated that topsoil addition significantly improved height and diameter growth, aerial and root biomasses, survival, and nutrient uptake (N, P, and S) in conifer seedlings, concomitantly to increased water content, decreased pH, and elevated nutrient concentrations in the substrate. However, multivariate analysis revealed that these improved soil conditions alone did not determine the survival and growth of conifer seedlings. In contrast, the application of RCW-based treatments had no discernible impact on the growth and survival of the planted trees. Additionally, topsoil addition effectively reduced the concentration of potentially phytotoxic elements in soil and needles, particularly arsenic. The total arsenic concentration in the mineral substrate (84.1 to 507 µg.g− 1) emerged as a growth-limiting factor for both conifer species. The total concentration of arsenic in the waste rock correlated positively with arsenic accumulation in the tree needles, indicating potential root uptake of this element. This study emphasizes the significance of addressing arsenic availability during reclamation efforts at mine sites. Nonetheless, further research is required to determine the phytotoxic thresholds of arsenic on conifers and its potential metabolic effects.