Construction and Investigation of a circRNA-Associated ceRNA Regulatory Network in Tetralogy of Fallot
Background: As the most frequent type of cyanotic congenital heart diseases (CHD), Tetralogy of Fallot (TOF) has a relatively poor prognosis without the corrective surgery. Circular RNA (circRNA) represents a novel class of endogenous noncoding RNAs that regulate target gene expression post-transcriptionally in heart development. Here, we investigate the potential role of ceRNA network in the pathogenesis of TOF.
Methods: To identify circRNAs expression profiles in Tetralogy of Fallot, microarrays were used to screen the differentially expressed circRNA between 3 TOF and 3 control human myocardial tissue samples. Then, a dysregulated circRNA- associated ceRNA network was constructed via using the established multi-step screening strategy.
Results: In summary, total 276 differentially expressed circRNAs were identified, including 214 up-regulated and 62 down-regulated ones in TOF samples. By constructing the circRNA-associated ceRNA network based on the bioinformatics data, a total of 19 key circRNAs, 9 key miRNAs and 34 key mRNAs were further screened. Moreover, by enlarging the samples size, the qPCR results validated that the positive correlations between hsa_circ_0007798 and HIF1A.
Conclusions: The findings in this study provide a comprehensive understanding of the ceRNA network involved in TOF biology, such as hsa_circ_0007798/miR-199b-
5p/HIF1A signal axis, and may offer candidate diagnostic biomarkers or potential therapeutic targets for TOF. In addtion, we propose that the ceRNA network regulates TOF progression.
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On 05 Jan, 2021
On 07 Dec, 2020
On 07 Dec, 2020
Received 07 Dec, 2020
Received 07 Dec, 2020
Invitations sent on 24 Nov, 2020
On 21 Nov, 2020
On 21 Nov, 2020
On 21 Nov, 2020
Posted 06 Jul, 2020
On 22 Sep, 2020
Received 19 Sep, 2020
On 01 Sep, 2020
Received 06 Aug, 2020
Received 06 Aug, 2020
On 20 Jul, 2020
On 18 Jul, 2020
Invitations sent on 09 Jul, 2020
On 02 Jul, 2020
On 01 Jul, 2020
On 30 Jun, 2020
On 30 Jun, 2020
Construction and Investigation of a circRNA-Associated ceRNA Regulatory Network in Tetralogy of Fallot
On 05 Jan, 2021
On 07 Dec, 2020
On 07 Dec, 2020
Received 07 Dec, 2020
Received 07 Dec, 2020
Invitations sent on 24 Nov, 2020
On 21 Nov, 2020
On 21 Nov, 2020
On 21 Nov, 2020
Posted 06 Jul, 2020
On 22 Sep, 2020
Received 19 Sep, 2020
On 01 Sep, 2020
Received 06 Aug, 2020
Received 06 Aug, 2020
On 20 Jul, 2020
On 18 Jul, 2020
Invitations sent on 09 Jul, 2020
On 02 Jul, 2020
On 01 Jul, 2020
On 30 Jun, 2020
On 30 Jun, 2020
Background: As the most frequent type of cyanotic congenital heart diseases (CHD), Tetralogy of Fallot (TOF) has a relatively poor prognosis without the corrective surgery. Circular RNA (circRNA) represents a novel class of endogenous noncoding RNAs that regulate target gene expression post-transcriptionally in heart development. Here, we investigate the potential role of ceRNA network in the pathogenesis of TOF.
Methods: To identify circRNAs expression profiles in Tetralogy of Fallot, microarrays were used to screen the differentially expressed circRNA between 3 TOF and 3 control human myocardial tissue samples. Then, a dysregulated circRNA- associated ceRNA network was constructed via using the established multi-step screening strategy.
Results: In summary, total 276 differentially expressed circRNAs were identified, including 214 up-regulated and 62 down-regulated ones in TOF samples. By constructing the circRNA-associated ceRNA network based on the bioinformatics data, a total of 19 key circRNAs, 9 key miRNAs and 34 key mRNAs were further screened. Moreover, by enlarging the samples size, the qPCR results validated that the positive correlations between hsa_circ_0007798 and HIF1A.
Conclusions: The findings in this study provide a comprehensive understanding of the ceRNA network involved in TOF biology, such as hsa_circ_0007798/miR-199b-
5p/HIF1A signal axis, and may offer candidate diagnostic biomarkers or potential therapeutic targets for TOF. In addtion, we propose that the ceRNA network regulates TOF progression.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6