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Research article
Lin Luo
Hunan University of Chinese Medicine
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1693-7469
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Neuroinflammation has been implicated in the pathogenesis of various neurological and cerebrovascular diseases. Recently, microglia has became a promising target to treat neuroinflammation-related diseases. BuyangHuanwu Decoction (BYHWD), a traditional Chinese medicine recipe, is a representative prescription for the treatment of neuroinflammation, showed great therapeutic potential in neuroinflammation-related cerebrovascular disorders including cerebral ischemia. However, it’s possible mechanism of action remains unclear.
Methods: In the present research, we studied the therapeutic effects of BYHWD on LPS-induced inflammatory response in BV2 microglia cells. The inflammatory cytokines include interleukin (IL-6), tumor necrosis factor (TNF-α) and nitric oxide (NO) were detected by commercial kit. The inflammatory-related protein was measured by western blot and immunofluorescence.
Results: Our findings showed that BYHWD could significantly reverse LPS-induced morphological changes in BV2 microglial and inhibit the local synthesis of proinflammatory cytokines and free radical via directly regulating NF-κB activity and inflammasome assembly. Our data showed that BYHWT inhibit LPS-induced TNF-α, IL-6, and NO production, Also can inhibit iNOS protein expression and the activation of NF-κB taccording to inhibit NLRP3 activation.
Conclusions: In conclusion, our results demonstrated that BYHWD is a potential candidate for treating neuroinflammation-related diseases. Certainly, more comprehensive researches on the precise mechanism of action of BYHWD in cerebrovascular disease is required in future vivo studies.
Keywords
BYHWD, Neuroinflammation, Oxidative stress, BV-2 cells, LPS
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This is a preprint. It has not completed peer review.
Research article
Lin Luo
Hunan University of Chinese Medicine
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1693-7469
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 30 Jun, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Neuroinflammation has been implicated in the pathogenesis of various neurological and cerebrovascular diseases. Recently, microglia has became a promising target to treat neuroinflammation-related diseases. BuyangHuanwu Decoction (BYHWD), a traditional Chinese medicine recipe, is a representative prescription for the treatment of neuroinflammation, showed great therapeutic potential in neuroinflammation-related cerebrovascular disorders including cerebral ischemia. However, it’s possible mechanism of action remains unclear.
Methods: In the present research, we studied the therapeutic effects of BYHWD on LPS-induced inflammatory response in BV2 microglia cells. The inflammatory cytokines include interleukin (IL-6), tumor necrosis factor (TNF-α) and nitric oxide (NO) were detected by commercial kit. The inflammatory-related protein was measured by western blot and immunofluorescence.
Results: Our findings showed that BYHWD could significantly reverse LPS-induced morphological changes in BV2 microglial and inhibit the local synthesis of proinflammatory cytokines and free radical via directly regulating NF-κB activity and inflammasome assembly. Our data showed that BYHWT inhibit LPS-induced TNF-α, IL-6, and NO production, Also can inhibit iNOS protein expression and the activation of NF-κB taccording to inhibit NLRP3 activation.
Conclusions: In conclusion, our results demonstrated that BYHWD is a potential candidate for treating neuroinflammation-related diseases. Certainly, more comprehensive researches on the precise mechanism of action of BYHWD in cerebrovascular disease is required in future vivo studies.
Figure 1
Figure 2
Figure 3
Figure 4
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