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Research Article
Teruhide Hoshino
Department of Oral Pathobiological Science and Surgery, Tokyo Dental College
Corresponding Author
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Frailty is a vulnerable state that marks the transition to long-term care for the elders. Recently, the relationship between frailty and oral function has been attracting attention. By clarifying the specific metabolic changes in the masseter muscle, we aimed to contribute to maintenance of masticatory function. The purpose of this study is to clarify the changes in masseter muscle of senescence-accelerated mouse-prone 8 (SAMP8) mice metabolites and metabolic pathways due to aging. Capillary electrophoresis-mass spectrometry metabolome analysis was performed on the masseter muscle of 12-week-old, 40-week-old, and 55-week-old mice. Expression analysis was performed by reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence for the metabolome pathways extracted by metabolome analysis that considered to be related to aging. Nineteen metabolites had a significant difference in absolute quantitative values and were considered to affect the first principal component by factor loading. The extracted metabolic pathways were glycolysis, polyamine metabolome pathway, and purine metabolome pathway. RT-PCR was performed on the extracted metabolome pathways. Expression of the spermidine synthase and hypoxanthine phosphoribosyl transferase genes with significant differences by RT-PCR was confirmed by immunofluorescence. The metabolic pathways considered to be related to aging in masseter muscle were glycolysis, polyamine metabolic pathway, and purine metabolic pathway.
Keywords
masseter muscle, senescence-accelerated mouse-prone 8 (SAMP8), frailty
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No competing interests reported.
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This is a preprint. It has not completed peer review.
Research Article
Teruhide Hoshino
Department of Oral Pathobiological Science and Surgery, Tokyo Dental College
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 27 Apr, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Frailty is a vulnerable state that marks the transition to long-term care for the elders. Recently, the relationship between frailty and oral function has been attracting attention. By clarifying the specific metabolic changes in the masseter muscle, we aimed to contribute to maintenance of masticatory function. The purpose of this study is to clarify the changes in masseter muscle of senescence-accelerated mouse-prone 8 (SAMP8) mice metabolites and metabolic pathways due to aging. Capillary electrophoresis-mass spectrometry metabolome analysis was performed on the masseter muscle of 12-week-old, 40-week-old, and 55-week-old mice. Expression analysis was performed by reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence for the metabolome pathways extracted by metabolome analysis that considered to be related to aging. Nineteen metabolites had a significant difference in absolute quantitative values and were considered to affect the first principal component by factor loading. The extracted metabolic pathways were glycolysis, polyamine metabolome pathway, and purine metabolome pathway. RT-PCR was performed on the extracted metabolome pathways. Expression of the spermidine synthase and hypoxanthine phosphoribosyl transferase genes with significant differences by RT-PCR was confirmed by immunofluorescence. The metabolic pathways considered to be related to aging in masseter muscle were glycolysis, polyamine metabolic pathway, and purine metabolic pathway.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
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