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Research
Lin Bai
Peking Union Medical College Comparative Medicine Center: Chinese Academy of Medical Sciences Institute of Laboratory Animal Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0646-0812
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background and Objectives
Adipose-tissue derived stem cells (ADSCs) autologous transplantation have been a promising strategy for aging-related disorder. But the relationship between ADSCs senescence and organismal aging were still no consistent conclusions. Toward this end, we analyzed the senescence properties of ADSCs from different age donors to furthermore understand the differences of cells between young and senile donors and verify the influence of organismal aging on the proliferation and function of ADSCs in vitro, providing the theoretical basis for the clinical application of autologous ADSCs transplantation.
Methods and Results
We detected the characteristics, function, gene expression, apoptosis, cell cycle, SA-β-gal staining, and transcription features of ADSCs from 1-month mice and 20-month mice. ADSCs from old donors had some senescence-associated changes with less ability to proliferation than ADSCs from 1-month mice. Differentiation ability, cell surface markers, and SA-β-Gal staining did not differ across donor age, while cells exhibit a more remarkable age-related changes through continuous passages. According to the results of transcriptome analysis, the CCL7-CCL2-CCR2 axis and Hippo signaling pathway would be considered as its possible mechanisms.
Conclusions
Our study reveals that ADSCs from old donors have some age-related alterations. The CCL7-CCL2-CCR2 which lies behind this change would be a potential target for gene therapy to reduce harmful effects of ADSCs from old donors. To make autologous transplantation work better, we would recommend that ADSCs should be cryopreserved in youth with minimum number of passages.
Keywords
ADSCs, Autologous stem cell transplantation, Aging, Chemokine, Cell cycle, Cell culture
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CURRENT STATUS: Under Review
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Posted 06 Apr, 2021
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Editorial decision: Major Revision
On 09 May, 2021
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Received 08 May, 2021
Review #2 received
Received 03 May, 2021
Review #3 received
Received 29 Apr, 2021
Reviewer #2 agreed
On 28 Apr, 2021
Reviewer #3 agreed
On 28 Apr, 2021
Reviewer #4 agreed
On 28 Apr, 2021
Reviewers invited
Invitations sent on 28 Apr, 2021
Reviewer #1 agreed
On 28 Apr, 2021
Editor assigned
On 02 Apr, 2021
Submission checks complete
On 02 Apr, 2021
Editor invited
On 02 Apr, 2021
First submitted
On 31 Mar, 2021
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Subject Areas
Stem Cell & Developmental Cell Biology
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This preprint is under consideration at Stem Cell Research & Therapy. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Lin Bai
Peking Union Medical College Comparative Medicine Center: Chinese Academy of Medical Sciences Institute of Laboratory Animal Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0646-0812
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 06 Apr, 2021
No community comments so far
Editorial decision: Major Revision
On 09 May, 2021
Review #1 received
Received 08 May, 2021
Review #2 received
Received 03 May, 2021
Review #3 received
Received 29 Apr, 2021
Reviewer #2 agreed
On 28 Apr, 2021
Reviewer #3 agreed
On 28 Apr, 2021
Reviewer #4 agreed
On 28 Apr, 2021
Reviewers invited
Invitations sent on 28 Apr, 2021
Reviewer #1 agreed
On 28 Apr, 2021
Editor assigned
On 02 Apr, 2021
Submission checks complete
On 02 Apr, 2021
Editor invited
On 02 Apr, 2021
First submitted
On 31 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Stem Cell & Developmental Cell Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background and Objectives
Adipose-tissue derived stem cells (ADSCs) autologous transplantation have been a promising strategy for aging-related disorder. But the relationship between ADSCs senescence and organismal aging were still no consistent conclusions. Toward this end, we analyzed the senescence properties of ADSCs from different age donors to furthermore understand the differences of cells between young and senile donors and verify the influence of organismal aging on the proliferation and function of ADSCs in vitro, providing the theoretical basis for the clinical application of autologous ADSCs transplantation.
Methods and Results
We detected the characteristics, function, gene expression, apoptosis, cell cycle, SA-β-gal staining, and transcription features of ADSCs from 1-month mice and 20-month mice. ADSCs from old donors had some senescence-associated changes with less ability to proliferation than ADSCs from 1-month mice. Differentiation ability, cell surface markers, and SA-β-Gal staining did not differ across donor age, while cells exhibit a more remarkable age-related changes through continuous passages. According to the results of transcriptome analysis, the CCL7-CCL2-CCR2 axis and Hippo signaling pathway would be considered as its possible mechanisms.
Conclusions
Our study reveals that ADSCs from old donors have some age-related alterations. The CCL7-CCL2-CCR2 which lies behind this change would be a potential target for gene therapy to reduce harmful effects of ADSCs from old donors. To make autologous transplantation work better, we would recommend that ADSCs should be cryopreserved in youth with minimum number of passages.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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