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Background
Sugarcane is an essential crop for sugar and ethanol production. Immediate processing of sugarcane is necessary after harvesting because of rapid sucrose losses and deterioration of stalks. This study was conducted to fill the knowledge gap regarding the exploration of fungal communities in harvested deteriorating sugarcane. Experiments were performed on simulating production at 30 ℃ and 40 ℃ after 0, 12, and 60 hours (h) of sugarcane harvesting and powder-processing.
Results
Both pH and sugar content declined significantly within 12 h. Fungal taxa were unraveled using ITS amplicon sequencing. With the increasing temperature, the diversity of the fungal community decreased over time. The fungal community structure significantly changed within 12 h of bagasse storage. Before storing, the dominant genus (species) in bagasse was Wickerhamomyces (W. anomalus). Following storage, at 30 ℃ and 40°C, Kazachstania (K. humilis) and Saccharomyces (S. cerevisiae) gradually grew, becoming abundant fungi. We found that the bagasse at different temperatures had a similar pattern after storage for the same intervals, indicating that the temperature was the primary cause for the variation of core features. Moreover, the impact of dominant fungal species on sugar content and pH of stored sugarcane juice was investigated. Also, the correlation between top fungal genera and significant environmental factors was assessed.
Conclusions
The study highlighted the importance of timeliness to refine sugar as soon as possible after harvesting the sugarcane crop. The lessons learned from this research are vital for sugarcane growers and the sugar industry for minimizing postharvest losses.
Figure 1
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Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.docx
Table S1 Sequence characteristics obtained in stored cane stalks. Fig S1. Sequence rarefaction curve CK: before storage (n= 3); s12h30c: stored 12h in 30℃ (n =3); s12h40c: stored 12h in 40℃ (n =3); s60h30c: stored 60h in 30℃ (n =3); s60h40c: stored 60h in 40℃ (n =3). Fig S2. The glucose and fructose profiles in sucrose medium of isolated strains cultured at 30℃and 40℃. (a) glucose at 30℃; (b) glucose at 40℃; (c) fructose at 30℃; (d) fructose at 40℃. Data are presented as the mean ± standard deviation.
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Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 07 Apr, 2021
No community comments so far
Editorial decision: Major revision
On 11 May, 2021
Review #2 received
Received 10 May, 2021
Reviewer #2 agreed
On 29 Apr, 2021
Reviews received
Received 29 Apr, 2021
Reviewer #1 agreed
On 07 Apr, 2021
Review #1 received
Received 07 Apr, 2021
Reviewers invited
Invitations sent on 07 Apr, 2021
Editor invited
On 04 Apr, 2021
Editor assigned
On 03 Apr, 2021
Submission checks complete
On 03 Apr, 2021
First submitted
On 03 Apr, 2021
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Subject Areas
Biotechnology and Bioengineering
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A new preprint design is coming!
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This preprint is under consideration at Biotechnology for Biofuels and Bioproducts. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 07 Apr, 2021
No community comments so far
Editorial decision: Major revision
On 11 May, 2021
Review #2 received
Received 10 May, 2021
Reviewer #2 agreed
On 29 Apr, 2021
Reviews received
Received 29 Apr, 2021
Reviewer #1 agreed
On 07 Apr, 2021
Review #1 received
Received 07 Apr, 2021
Reviewers invited
Invitations sent on 07 Apr, 2021
Editor invited
On 04 Apr, 2021
Editor assigned
On 03 Apr, 2021
Submission checks complete
On 03 Apr, 2021
First submitted
On 03 Apr, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biotechnology and Bioengineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Sugarcane is an essential crop for sugar and ethanol production. Immediate processing of sugarcane is necessary after harvesting because of rapid sucrose losses and deterioration of stalks. This study was conducted to fill the knowledge gap regarding the exploration of fungal communities in harvested deteriorating sugarcane. Experiments were performed on simulating production at 30 ℃ and 40 ℃ after 0, 12, and 60 hours (h) of sugarcane harvesting and powder-processing.
Results
Both pH and sugar content declined significantly within 12 h. Fungal taxa were unraveled using ITS amplicon sequencing. With the increasing temperature, the diversity of the fungal community decreased over time. The fungal community structure significantly changed within 12 h of bagasse storage. Before storing, the dominant genus (species) in bagasse was Wickerhamomyces (W. anomalus). Following storage, at 30 ℃ and 40°C, Kazachstania (K. humilis) and Saccharomyces (S. cerevisiae) gradually grew, becoming abundant fungi. We found that the bagasse at different temperatures had a similar pattern after storage for the same intervals, indicating that the temperature was the primary cause for the variation of core features. Moreover, the impact of dominant fungal species on sugar content and pH of stored sugarcane juice was investigated. Also, the correlation between top fungal genera and significant environmental factors was assessed.
Conclusions
The study highlighted the importance of timeliness to refine sugar as soon as possible after harvesting the sugarcane crop. The lessons learned from this research are vital for sugarcane growers and the sugar industry for minimizing postharvest losses.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.docx
Table S1 Sequence characteristics obtained in stored cane stalks. Fig S1. Sequence rarefaction curve CK: before storage (n= 3); s12h30c: stored 12h in 30℃ (n =3); s12h40c: stored 12h in 40℃ (n =3); s60h30c: stored 60h in 30℃ (n =3); s60h40c: stored 60h in 40℃ (n =3). Fig S2. The glucose and fructose profiles in sucrose medium of isolated strains cultured at 30℃and 40℃. (a) glucose at 30℃; (b) glucose at 40℃; (c) fructose at 30℃; (d) fructose at 40℃. Data are presented as the mean ± standard deviation.
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